Criblamydia sequanensis Harbors a Megaplasmid Encoding Arsenite Resistance

A novel TnMERI1-like transposon designated as TnMARS1 was identified from mercury resistant Bacilli isolated from Minamata Bay sediment. Two adjacent ars operon-like gene clusters, ars1 and ars2, flanked by a pair of 78-bp inverted repeat sequences, which resulted in a 13.8-kbp transposon-like fragment, were found to be sandwiched between two transposable genes of the TnMERI1-like transposon of a mercury resistant bacterium, Bacillus sp. MB24. The presence of a single transcription start site in each cluster determined by 5′-RACE suggested that both are operons. Quantitative real time RT-PCR showed that the transcription of the arsR genes contained in each operon was induced by arsenite, while arsR2 responded to arsenite more sensitively and strikingly than arsR1 did. Further, arsenic resistance complementary experiments showed that the ars2 operon conferred arsenate and arsenite resistance to an arsB-knocked out Bacillus host, while the ars1 operon only raised arsenite resistance slightly. This transposon nested in TnMARS1 was designated as TnARS1. Multi-gene cluster blast against bacteria and Bacilli whole genome sequence databases suggested that TnMARS1 is the first case of a TnMERI1-like transposon combined with an arsenic resistance transposon. The findings of this study suggested that TnMERI1-like transposons could recruit other mobile elements into its genetic structure, and subsequently cause horizontal dissemination of both mercury and arsenic resistances among Bacilli in Minamata Bay.

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INDUCTION AND MECHANISMS OF ARSENITE RESISTANCE IN PSEUDOMONAS PSEUDOMALLEI

Journal of Bacteriology ◽

10.1128/jb.88.1.143-150.1964 ◽

1964 ◽

Vol 88 (1) ◽

pp. 143-150 ◽

Cited By ~ 6

Author(s):

Kei Arima ◽

Michiko Beppu

Keyword(s):

Arsenite Resistance ◽

Pseudomonas Pseudomallei

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Arsenite Resistance in Leishmania and Possible Drug Targets

Advances In Experimental Medicine And Biology - Drug Targets in Kinetoplastid Parasites ◽

10.1007/978-0-387-77570-8_1 ◽

2008 ◽

pp. 1-8 ◽

Cited By ~ 6

Author(s):

Gaganmeet Singh ◽

K. G. Jayanarayan ◽

Chinmoy S. Dey

Keyword(s):

Drug Targets ◽

Arsenite Resistance

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High level arsenite resistance in Leishmania tarentolae is mediated by an active extrusion system

Molecular and Biochemical Parasitology ◽

10.1016/0166-6851(94)90095-7 ◽

1994 ◽

Vol 67 (1) ◽

pp. 49-57 ◽

Cited By ~ 76

Author(s):

Saibal Dey ◽

Barbara Papadopoulou ◽

Anass Haimeur ◽

Gaétan Roy ◽

Katherine Grondin ◽

...

Keyword(s):

Leishmania Tarentolae ◽

Arsenite Resistance ◽

High Level

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Arsenite resistance inListeria monocytogenes

Food Microbiology ◽

10.1016/0740-0020(91)90009-q ◽

1991 ◽

Vol 8 (2) ◽

pp. 161-166 ◽

Cited By ~ 8

Author(s):

R.L. Buchanan ◽

L.A. Klawitter ◽

S. Bhaduri ◽

H.G. Stahl

Keyword(s):

Arsenite Resistance

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Functional Analysis of Three Plasmids from Lactobacillus plantarum

Applied and Environmental Microbiology ◽

10.1128/aem.71.3.1223-1230.2005 ◽

2005 ◽

Vol 71 (3) ◽

pp. 1223-1230 ◽

Cited By ~ 70

Author(s):

Richard van Kranenburg ◽

Natasa Golic ◽

Roger Bongers ◽

Rob J. Leer ◽

Willem M. de Vos ◽

...

Keyword(s):

Functional Analysis ◽

Bacillus Subtilis ◽

Host Range ◽

Lactobacillus Plantarum ◽

Unknown Function ◽

Conjugative Plasmid ◽

Rolling Circle Replication ◽

Cloning Vectors ◽

Rolling Circle ◽

Arsenite Resistance

ABSTRACT Lactobacillus plantarum WCFS1 harbors three plasmids, pWCFS101, pWCFS102, and pWCFS103, with sizes of 1,917, 2,365, and 36,069 bp, respectively. The two smaller plasmids are of unknown function and contain replication genes that are likely to function via the rolling-circle replication mechanism. The host range of the pWCFS101 replicon includes Lactobacillus species and Lactococcus lactis, while that of the pWCFS102 replicon also includes Carnobacterium maltaromaticum and Bacillus subtilis. The larger plasmid is predicted to replicate via the theta-type mechanism. The host range of its replicon seems restricted to L. plantarum. Cloning vectors were constructed based on the replicons of all three plasmids. Plasmid pWCFS103 was demonstrated to be a conjugative plasmid, as it could be transferred to L. plantarum NC8. It confers arsenate and arsenite resistance, which can be used as selective markers.

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Interaction of FLASH with Arsenite Resistance Protein 2 Is Involved in Cell Cycle Progression at S Phase

Molecular and Cellular Biology ◽

10.1128/mcb.00289-09 ◽

2009 ◽

Vol 29 (17) ◽

pp. 4729-4741 ◽

Cited By ~ 45

Author(s):

Maria Kiriyama ◽

Yohei Kobayashi ◽

Motoki Saito ◽

Fuyuki Ishikawa ◽

Shin Yonehara

Keyword(s):

Cell Cycle Progression ◽

S Phase ◽

Terminal Deletion ◽

Cajal Bodies ◽

Deletion Mutants ◽

Amino Terminal ◽

Arsenite Resistance ◽

Resistance Protein ◽

Phase Progression ◽

Histone Transcription

ABSTRACT FLASH has been shown to be required for S phase progression and to interact with a nuclear protein, ataxia-telangiectasia locus (NPAT), a component of Cajal bodies in the nucleus and an activator of histone transcription. We investigated the role of human FLASH by using an inducible FLASH knockdown system in the presence or absence of various mutant forms of mouse FLASH. While carboxyl-terminal deletion mutants of FLASH, which do not interact with NPAT, can support S phase progression, its amino-terminal deletion mutants, which are unable to self associate, cannot support S phase progression, replication-dependent histone transcription, or the formation of Cajal bodies. Furthermore, FLASH was shown to be associated with arsenite resistance protein 2 (ARS2) through its central region, which is composed of only 13 amino acids. The expression of ARS2 and the interaction between FLASH and ARS2 are required for S phase progression. Taking these results together, FLASH functions in S phase progression through interaction with ARS2.

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