A Rare Case of Burkholderia Pseudomallie Presenting as Brain Abscess

Rationale: Meiliodosis is an extremely rare condition in Neurosurgery which is caused by the gram negative organism Burkholderia pseudomallie. Burkholderia pseudomallei (also known as Pseudomonas pseudomallei) is a Gram-negative, bipolar, aerobic, motile rod-shaped bacterium. It is a soil-dwelling bacterium endemic in tropical and subtropical regions worldwide, particularly in Thailand and northern Australia. It infects humans and animals and causes the disease melioidosis. It is also capable of infecting plants. If treatment is below par or delayed, this infection can produce diverse clinical symptoms and result in death. Patient Concerns: A 52 year old female, known case of Type 2 Diabetes mellitus on insulin, presented with complaints of a swelling in the left parieto-occipital region since 2 months & headache since 1 month. She had a past history of a fall from scooter 7 months ago, which then was not radiologically imaged. Patient was, otherwise, conscious, oriented and had no focal neurological deficit. Diagnosis: left parieto-occipital space occupying lesion confirmed by Ct brain and [mri + mrv] brain and culture and sensitivity of fluid aspirate showed moderate growth of burkholderia pseudomallie. Interventions: Intravenous Ceftazidime [1 g/12 h] was administered in-hospital for 4 weeks (Intensive Phase) and Oral Cotrimoxazole (Trimethoprim + sulfomethoxazole) {TAB.BACTRIM DS} twice daily was administered out-hospital (Maintenance Phase). Patient underwent Left parieto-occipital abscess drainage and debridement [Pus sent for Culture and Sensitivity] – Abscess drainage and removal of irregular thinned out bone around the skull defect + Biopsies sent from small extradural collection near the skull defect.Intravenous Ceftazidime was continued and discharged with Oral Cotrimoxazole (Trimethoprim + sulfomethoxazole).

POTENSI DAN PEMANFAATAN MIKROORGANISME DALAM PENGENDALIAN PENYAKIT TULAR NYAMUK

Beberapa nyamuk adalah vektor berbagai penyakit seperti demam berdarah dengue (DBD) dan malaria. Salah satu cara menurunkan angka penyakit tular nyamuk adalah dengan memutus rantai penularan dengan mencegah gigitan nyamuk. Pencegahan gigitan nyamuk sebagian besar memakai cara kimiawi baik menggunakan obat nyamuk bakar, repelen, maupun insektisida lainnya. Dampak negatif penggunaan insektisida kimia mendorong pengembangan dan penggunaan metode pengendalian nyamuk sebagai alternatif seperti pengendalian biologi. Pengendalian biologi dapat menggunakan mikroorganisme atau organisme yang berukuran kecil. Metode yang digunakan dalam penulisan adalah dengan menggunakan penelusuran literatur melalui buku, jurnal penelitian dan web page dengan rentang tahun 2010 sampai dengan bulan Agustus tahun 2019. Mikroorganisme golongan bakteri dan fungi dapat dijadikan sebagai agen pengendali biologi untuk pengendalian nyamuk terutama untuk larvasida. Mikroorganisme ini terdiri dari Bacillus thuringiensis, Bacillus sphaericus, Bacillus subtilis, Wolbachia pipientis, Bacillus mycoides, Klebsiella ozaenae, Pseudomonas pseudomallei, Beauveria bassiana dan Metarhizium anisopliae. Pengendalian nyamuk menggunakan mikroorganisme telah dilakukan pada genus Aedes, Culex, Anopheles dan Mansonia.

Efektifitas Bakteri Pseudomonas Sebagai Pengurai Bahan Organik (Protein,Karbohidrat,Lemak) Pada Media Air Limbah Pembenihan Ikan Lele Dumbo (Clarias sp.) Sistem Resirkulasi Tertutup [Effectiveness Of Pseudomonas Decomposing Organic Matter (Protein, Carbohydrate, Lipid) In Waste Water Of Dumbo Breeding Fish Catfish (Clarias sp.) Closed System Resirculation]

Raising fish rearing African catfish lately more and more attention due to its economical value, easy to maintain and grow quickly. Quality of water at seeding activities have received attention because of seed is a phase that is still vulnerable to environmental changes so that the replacement of water and absolutely must be done if the accumulation of organic materials that cause toxic. African catfish fish hatcheries closed recirculation system based on the work system of water management repeatedly by using a filter (filter) and decomposing bacteria. This study aimed to determine whether the bacterium Pseudomonas pseudomallei index of 97.81% sp, Pseudomonas stutzeri index 61.21% and 97.81% of Pseudomonas stutzeri index may decompose the organic material in an aqueous medium Clarias gariepinus hatchery closed recirculation system and how long required to decompose proteins, lipid and carbohidrat. This research method using descriptive methods, aims to describe symptoms that are based on data collected by observation. Factors include the observation of incubation time at the beginning of the experiment, day 3, day 6 and day-to-9 after incubation performed. This study uses four treatments, namely A (water catfish hatcheries closed recirculation system with the addition of the bacteria Pseudomonas pseudomallei index of 97.81%),B (catfish hatchery water recirculation system closed with the addition of the bacteria Pseudomonas stutzeri index of 97.81%), C (catfish hatchery water recirculation system closed with the addition of the bacteria Pseudomonas stutzeri index of 61.21% and D (catfish hatchery water recirculation system closed with the addition of Pseudomonas pseudomallei index of 97.81%, 97.81% index of Pseudomonas stutzeri and Pseudomonas stutzeri index of 61.21%). Result of this research shows that granting of Pseudomonas pseudomallei sp index 97.81%, 61.21% index of Pseudomonas stutzeri and Pseudomonas stutzeri index decreased 97.81% to give the effect of the proteins, lipid, carbohydrates, BOD and COD. The highest reduce protein content, lipid, carbohydrates, BOD and COD become to combination of pseudomonas bacteria after nine days.

Isolasi Bakteri Indigen Sebagai Pendegradasi Bahan Organik Pada Media Pembenihan Ikan Lele Dumbo (Clarias sp.) Sistem Resirkulasi Tertutup [Isolation Indigen Bacteria In Organic Materials As Media Degrading Seedling Fish Catfish (Clarias sp.) Closed Recirculation System]

Rearing of African catfish fry requires an abundant water supply, so this causes Clarias gariepinus hatchery operations difficult in areas that lack water, so use a closed recirculation system technology which is an attempt to tackle the problem of limited water in an area for hatchery operations. However, these systems have drawbacks which deposition of organic materials on water quality pond maintenance reduced the need for water quality management. Components of organic materials is a source of water quality pollutants consisting of proteins, lekam, and carbohydrates, so that in the organic material needed pendegradasian existence of groups of bacteria and proteolytic bacteria, lipolytic, and amylolitic. The results of this study show that in the media of African catfish hatchery fish have bacteria that degrade organic material that has properties of proteolytic, lipolytic, and most potentially amylolitic consisting of bacterial isolates of Pseudomonas stutzeri B ie (97.81%) with values highest protease hydrolysis test results 1.878 mm, ie G isolates of Pseudomonas pseudomallei (97.81%) with the highest value of 1.909 mm hydrolysis test and bacterial isolates of Pseudomonas stutzeri K (61.21%) with the highest value of 1.944 mm hydrolysis test.

Pengaruh Pemberian Beberapa Bakteri Terhadap Kelangsungan Hidup Benih Ikan Lele Dumbo (Clarias sp.) [The Effect Of Bacteria Supply To Survival Rate Juvenile Of African Catfish (Clarias sp.) ]

Commodities African catfish (Clarias sp) is a rapidly growing commodity in Indonesia. Clarias gariepinus is one of the leading commodities and have a good market. African catfish hatchery fish is generally done in areas that have abundant water resources so that a minimal constraint on the area of water. Therefore applied a closed system with the addition of bacteria recirculation degrading organic material that is expected to reduce the accumulation of organic material, there by increasing the survival of African catfish fry. This study aimed to investigate the influence of bacteria that degrade organic material in a closed recirculation system on the survival of fish fry of African catfish (Clarias sp.). This research using Completely Randomized Design with four treatments and five replications of each K treatment (0%), A (1%) (6.0x108 CFU / ml), B (3%) (1.8x109 CFU / ml ) and C (5%) (3.0x109). added bacteria consisting of Pseudomonas pseudomallei with similarity index (97.81%), Pseudomonas stutzeri with similarity index (97.81%), Pseudomonas stutzeri with similarity index ( 61.21%) The results of this study indicate that the addition of Pseudomonas pseudomallei (97.81%), Pseudomonas stutzeri (97.81%) and Pseudomonas stutzeri (61.21%) gave significant differences (p <0.05) against survival of fish fry of African catfish.

Eksplorasi Bakteri Indigen Pada Pembenihan Ikan Lele Dumbo (Clarias sp.) Sistem Resirkulasi Tertutup [Exploration Of Indigen Bacteria From Catfish (Clarias sp.) Breeding On Closed Resirculation System ]

The efforts of the catfish hatchery fish are generally confined to the central areas of hatchery that has abundant water resources. Solving the problem of limited water, appears a catfish hatchery system with a closed recirculation system. In such systems the process of biological filtration is the most important thing. It shows the handling of organic materials by utilizing the degrading bacteria is the key to successful management of the system. Thus necessary to be studied the role of bacteria degrading organic matter indigen (local bacteria) in the closed hatchery recirculation system of catfish. This study aimed to obtain bacterial strains degrading bacteria indigen as organic materials are very useful in improving water quality in the closed hatchery recirculation system of catfish and knowing the methods to the use of bacteria indigen as degrading organic matter. The method used in this study was designed based on the stages of research carried out in accordance with the objectives to be achieved. The results of the isolation and identification to the species level showed Pseudomonas stutzeri and Pseudomonas pseudomallei obtain the greatest value for hydrolysis index representing each trait protease, lipase and amylase. Bioremediation effectiveness test in vitro showed effective results in the treatment using consortia of bacteria inoculant. Consortia of bacteria inoculant effective in improving the growth rate and survival rate (SR) in the closed hatchery recirculation system of catfish.

IDENTIFIKASI SENYAWA KIMIA DAN AKTIVITAS ANTIBAKTERI EKSTRAK Piper Sp. ASAL PAPUA

The aim of this study was to identity the chemical compounds of the piper sp.Fruit originated from Papua, that has been used as “sirih pinang”(chew betle)and to determine the antibacterial activity of hexane and chloroform extraxt ofpiper sp. to Gram + bacteria isolates(Bacillus subtilis, Listeria monocytogenesdan Staphylococcus aureus) and Gram – bacteria isolates(Salmonellatyphimurium, Eschericia coli and Pseudomonas pseudomallei). The extractconcentration were 50 % and 25 %, and every treatment consisted of 3replications. The bacteriae growth media was Mueller Hinton Agar(MHA). Theresult of phytochemical screening of Piper sp.fruit(ether, alcohol,and waterextracts)showed that it contained esential oil, lipid,emodol,tannin,reducingsugar, anthrasenoid,polyuronide,glucocide,steroid glycoside.

EKSPLORASI BAKTERI KITINOLITIK DARI SAMPAH ORGANIK : ISOLASI DAN KARAKTRISASI ENZIM KITINASE

Enzim kitinase banyak digunakan dalam bidang medis, makanan, bioteknologi dan lingkungan. Banyaknya kebutuhan enzim kitinase menuntut penyediannya yang murah dan melimpah dengan teknologi produksi yang sederhana. Penelitian ini bertujuan untuk isolasi mikroba kitinolitik dari cairan fermentasi sampah organik, produksi dan uji aktivitas enzim kitinase serta mengetahui karakteristik dari enzim kitinase. Isolasi mikroba telah dilakukan dengan metode spread plate. Aktivitas kitinase ditentukan secara kualitatif dengan pengukuran indeks kitinolitik dan secara kuantitatif dengan pengukuran absorbansi menggunakan spektrofotometer Uv-Vis pada panjang gelombang 660 nm berdasarkan banyaknya substrat kitin yang dihidrolisis oleh enzim kitinase. Satu dari beberapa isolat yang didapatkan, yaitu isolat A1 menunjukkan aktivitas kitinolitik tertinggi, yaitu sebesar 1,21. Hasil identifikasi mikrobiologi menunjukkan bahwa isolat A1dinyatakan sebagai Pseudomonas pseudomallei. Bakteri ini mampu menghasilkan kitinase secara optimum pada jam ke 18 waktu fermentasi, dengan penambahan molase 0,5% (b/v) dan 1% kitin (b/v) pada media produksinya. Kitinase yang dihasilkan P. pseudomallei menunjukkan aktivitas optimum pada suhu 50 °C danpH sebesar 6.