Differential effect of immunoglobulin on the in vitro growth of several isolates of Plasmodium falciparum.

Using the human lymphoblastoid cell line, GM 4672, and PBL of Gambian adults immune to Plasmodium falciparum (Pf) malaria, we have produced human-human hybridomas and selected those that produce mAb against Pf antigens. The fusion frequency, using PWM-stimulated donor lymphocytes was between 6.8 X 10(-5) and 1.5 X 10(-6). Using immune fluorescence, immune precipitation, and Pf in vitro growth inhibition, we cloned four hybridomas that reacted with the Pf Mr 195,000 schizont/merozoite protein. The differences in proteins immune precipitated and in growth inhibition indicate that, during development of protective immunity against Pf malaria, a spectrum of antibodies is produced reacting with different epitopes on the same antigen. Only a portion of these antibodies exhibits biological activity, suggesting that the recognition of certain epitopes is required for the development of a protective immune response.

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Toxic effect of isolated glycophorin a on the in vitro growth of Plasmodium falciparum

Blut Zeitschrift für die gesamte Blutforschung ◽

10.1007/bf00321041 ◽

1987 ◽

Vol 54 (2) ◽

pp. 115-122 ◽

Cited By ~ 6

Author(s):

P. Hermentin ◽

G. Neunziger ◽

B. Enders ◽

W. Dahr

Keyword(s):

Plasmodium Falciparum ◽

Toxic Effect ◽

Glycophorin A ◽

In Vitro Growth

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Plasmodium Falciparum: Comparison of in Vitro Growth of Knobby and Knobless Isolates

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.1983.32.226 ◽

1983 ◽

Vol 32 (2) ◽

pp. 226-230 ◽

Cited By ~ 2

Author(s):

Mary R. Motyl ◽

Robert T. Reese

Keyword(s):

Plasmodium Falciparum ◽

In Vitro Growth

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Identification of Proteins from Plasmodium falciparum That Are Homologous to Reticulocyte Binding Proteins inPlasmodium vivax

Infection and Immunity ◽

10.1128/iai.69.2.1084-1092.2001 ◽

2001 ◽

Vol 69 (2) ◽

pp. 1084-1092 ◽

Cited By ~ 97

Author(s):

Tony Triglia ◽

Jenny Thompson ◽

Sonia R. Caruana ◽

Mauro Delorenzi ◽

Terry Speed ◽

...

Keyword(s):

Plasmodium Falciparum ◽

Red Blood Cells ◽

Molecular Mass ◽

Blood Cells ◽

Potential Role ◽

High Molecular Mass ◽

In Vitro Growth ◽

Merozoite Invasion ◽

Genome Databases

ABSTRACT Plasmodium falciparum infections can be fatal, whileP. vivax infections usually are not. A possible factor involved in the greater virulence of P. falciparum is that this parasite grows in red blood cells (RBCs) of all maturities whereasP. vivax is restricted to growth in reticulocytes, which represent only approximately 1% of total RBCs in the periphery. Two proteins, expressed at the apical end of the invasive merozoite stage from P. vivax, have been implicated in the targeting of reticulocytes for invasion by this parasite. A search of the P. falciparum genome databases has identified genes that are homologous to the P. vivax rbp-1 and -2 genes. Two of these genes are virtually identical over a large region of the 5′ end but are highly divergent at the 3′ end. They encode high-molecular-mass proteins of >300 kDa that are expressed in late schizonts and localized to the apical end of the merozoite. To test a potential role in merozoite invasion of RBCs, we analyzed the ability of these proteins to bind to mature RBCs and reticulocytes. No binding to mature RBCs or cell preparations enriched for reticulocytes was detected. We identified a parasite clone that lacks the gene for one of these proteins, showing that the gene is not required for normal in vitro growth. Antibodies to these proteins can inhibit merozoite invasion of RBCs.

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