scholarly journals Attachment of human and pig (K88) enterotoxigenic Escherichia coli strains to either human or porcine small intestinal cells.

1984 ◽  
Vol 45 (2) ◽  
pp. 522-524 ◽  
Author(s):  
C F Deneke ◽  
K McGowan ◽  
A D Larson ◽  
S L Gorbach
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Intestinal Cells ◽  
Small Intestinal

scholarly journals Enterotoxigenic Escherichia coli CS21 pilus contributes to adhesion to intestinal cells and to pathogenesis under in vivo conditions

Microbiology ◽  
2013 ◽  
Vol 159 (Pt_8) ◽  
pp. 1725-1735 ◽  
Author(s):  
C. P. Guevara ◽  
W. B. Luiz ◽  
A. Sierra ◽  
C. Cruz ◽  
F. Qadri ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Intestinal Cells

scholarly journals Human Mucosal IgA Immune Responses against Enterotoxigenic Escherichia coli

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 714
Author(s):  
Saman Riaz ◽  
Hans Steinsland ◽  
Kurt Hanevik
Keyword(s):  
Escherichia Coli ◽  
Vaccine Development ◽  
Enterotoxigenic Escherichia Coli ◽  
Secretory Iga ◽  
Small Intestinal Mucosa ◽  
Middle Income ◽  
Middle Income Countries ◽  
Human Volunteers ◽  
Iga Response ◽  
Small Intestinal

Infection with enterotoxigenic Escherichia coli (ETEC) is a major contributor to diarrheal illness in children in low- and middle-income countries and travelers to these areas. There is an ongoing effort to develop vaccines against ETEC, and the most reliable immune correlate of protection against ETEC is considered to be the small intestinal secretory IgA response that targets ETEC-specific virulence factors. Since isolating IgA from small intestinal mucosa is technically and ethically challenging, requiring the use of invasive medical procedures, several other indirect methods are used as a proxy for gauging the small intestinal IgA responses. In this review, we summarize the literature reporting on anti-ETEC human IgA responses observed in blood, activated lymphocyte assayss, intestinal lavage/duodenal aspirates, and saliva from human volunteers being experimentally infected with ETEC. We describe the IgA response kinetics and responder ratios against classical and noncanonical ETEC antigens in the different sample types and discuss the implications that the results may have on vaccine development and testing.

scholarly journals Positive correlation between the number of bacteria in soybean tempeh with the bioactivity of its extract against enterotoxigenic Escherichia coli (ETEC) adhesion to eukaryotic cells

Food Research ◽  
2021 ◽  
Vol 5 (6) ◽  
pp. 157-164
Author(s):  
T.E. Pramudito ◽  
G. Florencia ◽  
M.R. Gunawan ◽  
M.P. Pratiwi ◽  
Y. Yogiara
Keyword(s):  
Escherichia Coli ◽  
Statistical Analysis ◽  
Significant Positive Correlation ◽  
Enterotoxigenic Escherichia Coli ◽  
Eukaryotic Cells ◽  
Intestinal Cells ◽  
Bacterial Number ◽  
Agglutination Assay ◽  
Positive Correlation ◽  
Number Of Bacteria

Bioactive oligosaccharides from soybean tempeh can inhibit the adhesion of enterotoxigenic Escherichia coli (ETEC) to intestinal cells, thus reducing the severity of ETEC-mediated diarrhea. Bacteria are also present in tempeh but there has yet been any report regarding their effect on the anti-adhesion bioactivity of tempeh. In this research, the bacterial number in tempeh was quantified and the anti-adhesion bioactivity of tempeh extract was determined using yeast agglutination assay. Statistical analysis showed a moderately (R = 0.69) significant positive correlation (P < 0.01) between the number of bacteria in tempeh and the anti-adhesion bioactivity of its extract. In conclusion, tempeh that contains more bacteria is more effective in inhibiting ETEC adhesion to eukaryotic cells. This could be due to a symbiosis between Rhizopus and bacteria in breaking down soy matrix polysaccharides to release bioactive oligosaccharides.

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