Effect of different plant extracts and natural substances (PENS) against membrane damage induced by enterotoxigenic Escherichia coli K88 in pig intestinal cells

2007 ◽  
Vol 21 (2) ◽  
pp. 224-229 ◽  
Author(s):  
M. Roselli ◽  
M.S. Britti ◽  
I. Le Huërou-Luron ◽  
H. Marfaing ◽  
W.Y. Zhu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Plant Extracts ◽  
Membrane Damage ◽  
Enterotoxigenic Escherichia Coli ◽  
Intestinal Cells ◽  
Natural Substances

scholarly journals Enterotoxigenic Escherichia coli CS21 pilus contributes to adhesion to intestinal cells and to pathogenesis under in vivo conditions

Microbiology ◽  
2013 ◽  
Vol 159 (Pt_8) ◽  
pp. 1725-1735 ◽  
Author(s):  
C. P. Guevara ◽  
W. B. Luiz ◽  
A. Sierra ◽  
C. Cruz ◽  
F. Qadri ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Intestinal Cells

scholarly journals Positive correlation between the number of bacteria in soybean tempeh with the bioactivity of its extract against enterotoxigenic Escherichia coli (ETEC) adhesion to eukaryotic cells

Food Research ◽  
2021 ◽  
Vol 5 (6) ◽  
pp. 157-164
Author(s):  
T.E. Pramudito ◽  
G. Florencia ◽  
M.R. Gunawan ◽  
M.P. Pratiwi ◽  
Y. Yogiara
Keyword(s):  
Escherichia Coli ◽  
Statistical Analysis ◽  
Significant Positive Correlation ◽  
Enterotoxigenic Escherichia Coli ◽  
Eukaryotic Cells ◽  
Intestinal Cells ◽  
Bacterial Number ◽  
Agglutination Assay ◽  
Positive Correlation ◽  
Number Of Bacteria

Bioactive oligosaccharides from soybean tempeh can inhibit the adhesion of enterotoxigenic Escherichia coli (ETEC) to intestinal cells, thus reducing the severity of ETEC-mediated diarrhea. Bacteria are also present in tempeh but there has yet been any report regarding their effect on the anti-adhesion bioactivity of tempeh. In this research, the bacterial number in tempeh was quantified and the anti-adhesion bioactivity of tempeh extract was determined using yeast agglutination assay. Statistical analysis showed a moderately (R = 0.69) significant positive correlation (P < 0.01) between the number of bacteria in tempeh and the anti-adhesion bioactivity of its extract. In conclusion, tempeh that contains more bacteria is more effective in inhibiting ETEC adhesion to eukaryotic cells. This could be due to a symbiosis between Rhizopus and bacteria in breaking down soy matrix polysaccharides to release bioactive oligosaccharides.

scholarly journals Characterization of egg yolk immunoglobulin (IgY) against enterotoxigenic Escherichia coli and evaluation of its effects on bovine intestinal cells

2013 ◽  
Vol 7 (5) ◽  
pp. 398-405 ◽  
Author(s):  
Valeria Bellingeri Romina ◽  
Busso Lucila ◽  
Eduardo Alustiza Fabrisio ◽  
Yanina Picco Natalia ◽  
Paola Molinero Daniela ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Egg Yolk ◽  
Enterotoxigenic Escherichia Coli ◽  
Intestinal Cells ◽  
Egg Yolk Immunoglobulin

scholarly journals Maillard neoglycans as inhibitors for in vitro adhesion of F4+ enterotoxigenic Escherichia coli to piglet intestinal cells

2017 ◽  
Vol 64 (4) ◽  
pp. 679-686 ◽  
Author(s):  
Héctor Manuel Sarabia-Sainz ◽  
Verónica Mata Haro ◽  
José Andre-i Sarabia Sainz ◽  
Luz Vázquez-Moreno ◽  
Gabriela Ramos-Clamont Montfort
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Intestinal Cells ◽  
In Vitro Adhesion

scholarly journals Attachment of enterotoxigenic Escherichia coli to human intestinal cells.

1983 ◽  
Vol 39 (3) ◽  
pp. 1102-1106 ◽  
Author(s):  
C F Deneke ◽  
K McGowan ◽  
G M Thorne ◽  
S L Gorbach
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Intestinal Cells ◽  
Human Intestinal Cells

Zinc oxide inhibits enterotoxigenic Escherichia coli K88 growth in culture and reduces the inflammatory response of porcine intestinal epithelial cells to infection

2009 ◽  
Vol 2009 ◽  
pp. 32-32
Author(s):  
H Sargeant ◽  
M-A Shaw ◽  
H M Miller
Keyword(s):  
Escherichia Coli ◽  
Zinc Oxide ◽  
Intestinal Epithelial Cells ◽  
Innate Immune ◽  
Enterotoxigenic Escherichia Coli ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Genomic Study ◽  
Etec Infection

Pharmacological levels of zinc oxide in the post-weaning piglet diet reduce the incidence and severity of diarrhoea, in particular that caused by enterotoxigenic Escherichia coli (ETEC) K88 (Owusu-Asiedu et al. 2003). A previous in vivo genomic study (Sargeant et al, 2008) identified several genes differentially expressed in the small intestine of ETEC-challenged piglets when fed a zinc oxide-supplemented diet. This included decreased expression of several genes involved in the inflammatory and innate immune response. It has been reported that ZnO reduces adhesion and internalisation of K88 to cultured human enterocytes, counteracting the up-regulation of inflammatory cytokines caused by ETEC infection. However, this effect was not due to growth inhibition of ETEC K88 in ZnO (Roselli et al, 2003). The objective of this study was to determine whether zinc oxide shows the same mode of action in protecting porcine intestinal cells against ETEC K88 as has been demonstrated in human cells, providing an explanation for in vivo findings.

Sign in / Sign up

Export Citation Format

Share Document