scholarly journals Positive correlation between the number of bacteria in soybean tempeh with the bioactivity of its extract against enterotoxigenic Escherichia coli (ETEC) adhesion to eukaryotic cells

Food Research ◽  
2021 ◽  
Vol 5 (6) ◽  
pp. 157-164
Author(s):  
T.E. Pramudito ◽  
G. Florencia ◽  
M.R. Gunawan ◽  
M.P. Pratiwi ◽  
Y. Yogiara
Keyword(s):  
Escherichia Coli ◽  
Statistical Analysis ◽  
Significant Positive Correlation ◽  
Enterotoxigenic Escherichia Coli ◽  
Eukaryotic Cells ◽  
Intestinal Cells ◽  
Bacterial Number ◽  
Agglutination Assay ◽  
Positive Correlation ◽  
Number Of Bacteria

Bioactive oligosaccharides from soybean tempeh can inhibit the adhesion of enterotoxigenic Escherichia coli (ETEC) to intestinal cells, thus reducing the severity of ETEC-mediated diarrhea. Bacteria are also present in tempeh but there has yet been any report regarding their effect on the anti-adhesion bioactivity of tempeh. In this research, the bacterial number in tempeh was quantified and the anti-adhesion bioactivity of tempeh extract was determined using yeast agglutination assay. Statistical analysis showed a moderately (R = 0.69) significant positive correlation (P < 0.01) between the number of bacteria in tempeh and the anti-adhesion bioactivity of its extract. In conclusion, tempeh that contains more bacteria is more effective in inhibiting ETEC adhesion to eukaryotic cells. This could be due to a symbiosis between Rhizopus and bacteria in breaking down soy matrix polysaccharides to release bioactive oligosaccharides.

scholarly journals Enterotoxigenic Escherichia coli CS21 pilus contributes to adhesion to intestinal cells and to pathogenesis under in vivo conditions

Microbiology ◽  
2013 ◽  
Vol 159 (Pt_8) ◽  
pp. 1725-1735 ◽  
Author(s):  
C. P. Guevara ◽  
W. B. Luiz ◽  
A. Sierra ◽  
C. Cruz ◽  
F. Qadri ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Intestinal Cells

scholarly journals Virulence profiles of enterotoxigenic Escherichia coli isolated from piglets with post-weaning diarrhea and classification according to fecal consistency

2016 ◽  
Vol 36 (4) ◽  
pp. 253-257 ◽  
Author(s):  
José Paulo H. Sato ◽  
Karine L. Takeuti ◽  
Mariana R. Andrade ◽  
Priscilla K.V. Koerich ◽  
Vinícius Tagliari ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Statistical Analysis ◽  
Virulence Factors ◽  
Molecular Techniques ◽  
Enterotoxigenic Escherichia Coli ◽  
Southern Brazil ◽  
Weaned Piglets ◽  
Bacterial Isolation ◽  
E Coli

Abstract The aim of this study was to assess the frequency and association of virulence factors of Escherichia (E.) coli isolated from weaned piglets with diarrhea and to correlate it with fecal consistency. A total of 152 rectal swabs were collected from 25-40 day-old piglets with diarrhea, in farms of Southern Brazil. Phenotypical and molecular techniques were used for bacterial isolation, characterization and classification of enterotoxigenic E. coli (ETEC) pathotypes. Statistical analysis was carried out to determine the frequency of virulence factors and virotypes, of fimbriae F4, F5, F6, F18, F41 and toxins LT, STa, STb and STx2e. Out of 456 E. coli isolates, 287 (62.9%) samples showed significant growth of E. coli. Among them, 194 (67.6%) samples showed at least one virulence factor, indicating that ETEC is an important etiological agent of diarrhea in weaned piglets. Higher frequencies were found of fimbria F4 and F18 and enterotoxins LT, STa and STb. Significant association was found to F4, LT, STa and STb; between F18 and STa and STx2e; between F5 and LT, STa and STb. The most frequent virotypes were F18-STa, F4-LT-STa-STb, F4-STa, F4-LT-STb and F18-STa-STx2e. Beta-hemolysis was observed in 47.4% of samples and there was significant association between hemolytic samples and virulence factors F4, F18, STa and STx2e. Regarding fecal consistency, there was significant association of liquid feces and F4 fimbria, STa toxin and virotypes F4-STa and F4-F5-LT-STa-STb. Since there was significant association of ETEC and liquid feces in nursery piglets, it is important to prioritize the sampling of liquid feces for the diagnosis etiologic cause of diarrhea.

scholarly journals Characterization of egg yolk immunoglobulin (IgY) against enterotoxigenic Escherichia coli and evaluation of its effects on bovine intestinal cells

2013 ◽  
Vol 7 (5) ◽  
pp. 398-405 ◽  
Author(s):  
Valeria Bellingeri Romina ◽  
Busso Lucila ◽  
Eduardo Alustiza Fabrisio ◽  
Yanina Picco Natalia ◽  
Paola Molinero Daniela ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Egg Yolk ◽  
Enterotoxigenic Escherichia Coli ◽  
Intestinal Cells ◽  
Egg Yolk Immunoglobulin

scholarly journals Maillard neoglycans as inhibitors for in vitro adhesion of F4+ enterotoxigenic Escherichia coli to piglet intestinal cells

2017 ◽  
Vol 64 (4) ◽  
pp. 679-686 ◽  
Author(s):  
Héctor Manuel Sarabia-Sainz ◽  
Verónica Mata Haro ◽  
José Andre-i Sarabia Sainz ◽  
Luz Vázquez-Moreno ◽  
Gabriela Ramos-Clamont Montfort
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Intestinal Cells ◽  
In Vitro Adhesion

scholarly journals Attachment of enterotoxigenic Escherichia coli to human intestinal cells.

1983 ◽  
Vol 39 (3) ◽  
pp. 1102-1106 ◽  
Author(s):  
C F Deneke ◽  
K McGowan ◽  
G M Thorne ◽  
S L Gorbach
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Intestinal Cells ◽  
Human Intestinal Cells
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