scholarly journals Identification of plasmid-encoded mannose-resistant hemagglutinin and HEp-2 and HeLa cell adherence factors of two diarrheagenic Escherichia coli strains belonging to an enteropathogenic serogroup.

1990 ◽  
Vol 58 (4) ◽  
pp. 1106-1113 ◽  
Author(s):  
R Pal ◽  
A C Ghose
Keyword(s):  
Escherichia Coli ◽  
Hela Cell ◽  
Cell Adherence ◽  
Diarrheagenic Escherichia Coli

Use of DNA Probes and HEp-2 Cell Adherence Assay to Detect Diarrheagenic Escherichia coli

1988 ◽  
Vol 158 (1) ◽  
pp. 224-228 ◽  
Author(s):  
M. M. Levine ◽  
V. Prado ◽  
R. Robins-Browne ◽  
H. Lior ◽  
J. B. Kaper ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dna Probes ◽  
Cell Adherence ◽  
Diarrheagenic Escherichia Coli ◽  
Adherence Assay

scholarly journals Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria

2015 ◽  
Vol 9 (02) ◽  
pp. 165-174 ◽  
Author(s):  
Casmir Ifeanyichukwu Cajetan Ifeanyi ◽  
Nkiruka Florence Ikeneche ◽  
Bassey Enya Bassey ◽  
Nazek Al-Gallas ◽  
Ridha Ben Aissa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Genes ◽  
Diarrheal Disease ◽  
Cell Adherence ◽  
Chain Reaction ◽  
E Coli ◽  
Atypical Epec ◽  
Diarrheagenic Escherichia Coli ◽  
Stool Specimens ◽  
Polymerase Chain

Introduction: Escherichia coli are frequently isolated from diarrheic children in the Federal Capital Territory Abuja, Nigeria, but their virulent properties are not routinely evaluated. Therefore, the etiology of childhood diarrheal disease attributable to diarrheagenic Escherichia coli (DEC) in Abuja, Nigeria remains unknown. Methodology: Stool specimens from 400 acute diarrheic children between 0 and 60 months of age were studied.E. coli strains isolated were evaluated by polymerase chain reaction (PCR) for nine virulence genes and HEp-2 cell adherence to detect and identify five distinct diarrheagenic E. coli categories. Results: Diarrheagenic E.coli was detected in 51 (12.8%) of the diarrheic children. The observed DEC pathotypes were enteropathogenic E. coli (EPEC) in 18 (4.5%) children, enterotoxigenic E. coli (ETEC) in 16 (4.0%), enteroaggrative E. coli (EAEC) in 8 (2.0%), enterohaemorrhagic E. coli (EHEC) in 6 (1.5%), and enteroinvasive E. coli (EIEC) in 3 (0.8%). Four (1.0 %) EPEC strains with only the eae+ gene that adhered diffusely to HEp-2 cell were identified as atypical EPEC. All the DEC categories except atypical EPEC were identified in children between 6 and 12 months of age. Conclusions: This study underscores the need for routine evaluation of diarrheic children for virulence properties of infectious DEC. Atypical EPEC are emerging among the DEC pathotypes isolated from childhood acute gastroenteritis in Abuja, Nigeria.

scholarly journals The importance of P and type 1 fimbriae for the persistence ofEscherichia coliin the human gut

1992 ◽  
Vol 108 (3) ◽  
pp. 415-421 ◽  
Author(s):  
K. Tullus ◽  
I. Kühn ◽  
I. Ørskov ◽  
F. Ørskov ◽  
R. Möllby
Keyword(s):  
Escherichia Coli ◽  
Hela Cell ◽  
Hela Cells ◽  
Human Intestine ◽  
Cell Adherence ◽  
Human Gut ◽  
E Coli ◽  
Type 1 Fimbriae ◽  
Faecal Samples

SUMMARYThe faecalEscherichia coliflora was studied in 89 infants. Each infant was followed with a mean of 12 faecal samples (range 5–21) between 0 and 18 months of age. All isolates were assayed for P fimbriae and biochemically phenotyped and the persistence of each strain (phenotype) in the infant's gut was determined. In a subset of strains the occurrence of type 1 fimbriae and adherence to HeLa cells was studied. Thirty-one per cent of isolates belonging to strains colonizing for longer than 6 months expressed P fimbriae compared to 19% of the isolates from strains colonizing 1–6 months or transient strains colonizing less than 1 month. Type 1 fimbriae and adherence to HeLa cells occurred similarly often in all groups of strains. We conclude that P fimbriae, but not type 1 fimbriae or HeLa cell adherence seemed to contribute to the ability of theE. colistrain to colonize the human intestine.

HeLa cell adherence and cytotoxin production by enteropathogenic Escherichia coli isolated from infants with diarrhea in Thailand.

1987 ◽  
Vol 25 (8) ◽  
pp. 1519-1523 ◽  
Author(s):  
P Echeverria ◽  
D N Taylor ◽  
A Donohue-Rolfe ◽  
K Supawat ◽  
O Ratchtrachenchai ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hela Cell ◽  
Enteropathogenic Escherichia Coli ◽  
Cell Adherence

Usefulness of a Multiplex PCR for Detection of Diarrheagenic Escherichia coli in a Diagnostic Microbiology Laboratory Setting

2016 ◽  
Vol 1 (2) ◽  
pp. 38-42 ◽  
Author(s):  
Khairun Nessa ◽  
Dilruba Ahmed ◽  
Johirul Islam ◽  
FM Lutful Kabir ◽  
M Anowar Hossain
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Clinical Laboratory ◽  
Proteinase K ◽  
Microbiology Laboratory ◽  
Pcr Assay ◽  
E Coli ◽  
Diarrheagenic Escherichia Coli ◽  
Multiplex Pcr Assay ◽  
Diagnostic Microbiology

A multiplex PCR assay was evaluated for diagnosis of diarrheagenic Escherichia coli in stool samples of patients with diarrhoea submitted to a diagnostic microbiology laboratory. Two procedures of DNA template preparationproteinase K buffer method and the boiling method were evaluated to examine isolates of E. coli from 150 selected diarrhoeal cases. By proteinase K buffer method, 119 strains (79.3%) of E. coli were characterized to various categories by their genes that included 55.5% enteroaggregative E. coli (EAEC), 18.5% enterotoxigenic E. coli (ETEC), 1.7% enteropathogenic E. coli (EPEC), and 0.8% Shiga toxin-producing E. coli (STEC). Although boiling method was less time consuming (<24 hrs) and less costly (<8.0 US $/ per test) but was less efficient in typing E. coli compared to proteinase K method (41.3% vs. 79.3% ; p<0.001). The sensitivity and specificity of boiling method compared to proteinase K method was 48.7% and 87.1% while the positive and negative predictive value was 93.5% and 30.7%, respectively. The majority of pathogenic E. coli were detected in children (78.0%) under five years age with 53.3% under one year, and 68.7% of the children were male. Children under 5 years age were frequently infected with EAEC (71.6%) compared to ETEC (24.3%), EPEC (2.7%) and STEC (1.4%). The multiplex PCR assay could be effectively used as a rapid diagnostic tool for characterization of diarrheagenic E. coli using a single reaction tube in the clinical laboratory setting.Bangladesh J Med Microbiol 2007; 01 (02): 38-42

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