Peritoneal Culture Alters Streptococcus pneumoniae Protein Profiles and Virulence Properties
2000 ◽
Vol 68
(10)
◽
pp. 6082-6086
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Keyword(s):
ABSTRACT We have examined the properties of Streptococcus pneumoniae cultured in the murine peritoneal cavity and compared its virulence-associated characteristics to those of cultures grown in vitro. Analysis of mRNA levels for specific virulence factors demonstrated a 2.8-fold increase in ply expression and a 2.2-fold increase in capA3 expression during murine peritoneal culture (MPC). Two-dimensional gels and immunoblots using convalescent-phase patient sera and murine sera revealed distinct differences in protein production in vivo (MPC). MPC-grown pneumococci adhered to A549 epithelial cell lines at levels 10-fold greater than those cultured in vitro.
In Vivo and in Vitro Analysis of Lysosomes and Acid Phosphatase Activity in Human Chagasic Placentas
1995 ◽
Vol 63
(3)
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pp. 153-160
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Keyword(s):
1995 ◽
Vol 752
(1 Cardiac Growt)
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pp. 370-386
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Keyword(s):
2008 ◽
Vol 53
(2)
◽
pp. 541-551
◽
Keyword(s):
2010 ◽
Vol 468
(10)
◽
pp. 2695-2703
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