scholarly journals Dissemination of the Phage-Associated Novel Superantigen Gene speL in Recent Invasive and Noninvasive Streptococcus pyogenes M3/T3 Isolates in Japan

2002 ◽  
Vol 70 (6) ◽  
pp. 3227-3233 ◽  
Author(s):  
Tadayoshi Ikebe ◽  
Akihito Wada ◽  
Yoshishige Inagaki ◽  
Kumiko Sugama ◽  
Rieko Suzuki ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Streptococcus Pyogenes ◽  
Phage Particle ◽  
Attachment Site ◽  
Temperate Phage ◽  
Invasive Infection ◽  
Particle Fraction ◽  
Toxic Shock ◽  
Terminal Part ◽  
Streptococcus Equi

ABSTRACT In Japan, more than 10% of streptococcal toxic shock-like syndrome (TSLS) cases have been caused by Streptococcus pyogenes M3/T3 isolates since the first reported TSLS case in 1992. Most M3/T3 isolates from TSLS or severe invasive infection cases during 1992 to 2001 and those from noninvasive cases during this period are indistinguishable in pulsed-field gel electropherograms. The longest fragments of these recent isolates were 300 kb in size, whereas those of isolates recovered during or before 1973 were 260 kb in size. These 260- and 300-kb fragments hybridized to each other, suggesting the acquisition of an about 40-kb fragment by the recent isolates. The whole part of the acquired fragment was cloned from the first Japanese TSLS isolate, NIH1, and its nucleotide sequence was determined. The 41,796-bp fragment is temperate phage φNIH1.1, containing a new superantigen gene speL near its right attachment site. The C-terminal part of the deduced amino acid sequence of speL has 48 and 46% similarity with well-characterized erythrogenic toxin SpeC and the most potent superantigen, SmeZ-2, respectively. None of 10 T3 isolates recovered during or before 1973 has speL, whereas all of 18 M3/T3 isolates recovered during or after 1992 and, surprisingly, Streptococcus equi subsp. equi ATCC 9527 do have this gene. Though plaques could not be obtained from φNIH1.1, its DNA became detectable from the phage particle fraction upon mitomycin C induction, showing that this phage is not defective. A horizontal transfer of the phage carrying speL may explain the observed change in M3/T3 S. pyogenes isolates in Japan.

scholarly journals Spontaneous mutations in Streptococcus pyogenes isolates from streptococcal toxic shock syndrome patients play roles in virulence

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Tadayoshi Ikebe ◽  
Takayuki Matsumura ◽  
Hisako Nihonmatsu ◽  
Hitomi Ohya ◽  
Rumi Okuno ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Toxic Shock Syndrome ◽  
Streptococcal Toxic Shock Syndrome ◽  
Spontaneous Mutations ◽  
Toxic Shock

scholarly journals Gastrointestinal Mucormycosis following aStreptococcus pyogenesToxic Shock Syndrome in a Previously Healthy Patient: A Case Report

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Jean-François Roussy ◽  
Catherine Allard ◽  
Guy St-Germain ◽  
Jacques Pépin
Keyword(s):  
Case Report ◽  
Opportunistic Infection ◽  
Streptococcus Pyogenes ◽  
Toxic Shock Syndrome ◽  
Immunocompromised Patients ◽  
Healthy Patient ◽  
Toxic Shock ◽  
Rhizopus Microsporus ◽  
First Case ◽  
Rhizopus Sp

Mucormycosis is an uncommon opportunistic infection and the gastrointestinal form is the rarest.Rhizopus sp. is the most frequent pathogen and infection occurs almost exclusively in immunocompromised patients. We describe the first case of intestinal mucormycosis occurring after aStreptococcus pyogenestoxic shock syndrome in a previously healthy patient caused byRhizopus microsporusvar.azygosporus.

scholarly journals Genetic characterization of Streptococcus pyogenes emm 89 strains isolated in Japan from 2011 to 2019

2020 ◽  
Author(s):  
Yujiro Hirose ◽  
Masaya Yamaguchi ◽  
Norihiko Takemoto ◽  
Tohru Miyoshi-Akiyama ◽  
Tomoko Sumitomo ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Genetic Characterization ◽  
Streptococcal Toxic Shock Syndrome ◽  
Toxic Shock ◽  
Gene Encoding ◽  
Serum Opacity Factor ◽  
Genetic Features ◽  
Fibronectin Binding Protein

Streptococcal toxic shock syndrome (STSS) caused by Streptococcus pyogenes emm 89 strains has been increasing in several countries and reported to be linked with a recently emerged clade of emm89 strains, designated clade 3. In Japan, epidemiological and genetic information for emm89 strains remains elusive. In this study, we utilized emm89 strains isolated from both STSS (89 isolates) and non-STSS (72 isolates) infections in Japan from 2011 to 2019, and conducted whole-genome sequencing and comparative analysis, which resulted in classification of a large majority into clade 3 regardless of disease severity. In addition, STSS-associated genes and SNPs were found in clade 3 strains, including mutations of streptokinase (Ska), control of virulence sensor (CovS), serum opacity factor (SOF), sortase (SrtB), and fibronectin-binding protein F1 (PrtF1), and absence of the hylP1 gene encoding hyaluronidase. These findings provide insights into notable genetic features of emm89 strains.

Fulminant infection and toxic shock syndrome caused by Streptococcus pyogenes

2002 ◽  
Vol 22 (4) ◽  
pp. 357-366 ◽  
Author(s):  
Kevin L Fox ◽  
Michael W Born ◽  
Merrill A Cohen
Keyword(s):  
Streptococcus Pyogenes ◽  
Toxic Shock Syndrome ◽  
Toxic Shock

scholarly journals Pemanfaatan Buah Belimbing Wuluh (Averrhoa bilimbi L.) Sebagai Alternatif Penanganan Toxic Shock Syndrome

2020 ◽  
pp. 73 ◽  
Author(s):  
Suly Sunarsi
Keyword(s):  
Staphylococcus Aureus ◽  
Streptococcus Pyogenes ◽  
Toxic Shock Syndrome ◽  
Stevia Rebaudiana ◽  
Toxic Shock

Toxic Shock Syndrome (TSS) merupakan penyakit akut yang mengancam jiwa yang dimediasi oleh racun, biasanya dipicu oleh infeksi Staphylococcus aureus atau grup A Streptococcus (GAS), diantaranya Streptococcus pyogenes. Pemanfaatan buah belimbing wuluh masih terbatas dibandingkan dengan ketersediannya di kalangan masyarakat, sehingga penelitian ini bermaksud untuk memanfaatkan belimbing wuluh yang diformulasi dalam bentuk Infused Water dengan pemanis alami Stevia rebaudiana yang digunakan sebagai alternatif penanganan Toxic Shock Syndrome dengan penghambatan pada bakteri Streptococcus pyogenes. Sari buah belimbing wuluh dan liofilisatnya diujikan terhadap Streptococcus pyogenes secara In Vitro dengan beberapa variasi konsentrasi untuk melihat aktivitas antibakterinya. Infused water buah belimbing wuluh dibuat dengan metode perendaman selama 12 jam dan 24 jam dengan kombinasi 500 mg daun stevia dalam 250 mL air matang dan dievaluasi menggunakan uji organoleptik, uji ALT dan uji MPN. Hasil penelitian menunjukkan uji aktivitas antibakteri sari buah belimbing wuluh terdapat zona hambat dengan diameter 7,09±0,2 (1%), 7,41±0,1 (3%), 8,33±0,3 (5%), dan 8,38±0,2 (10%). Sedangkan liofilisat sari buah belimbing wuluh menunjukkan zona hambat dengan diameter 7,11±0,4 (1%), 7,34±0,3 (3%), 7,05±0,8 (5%), dan 7,44±0,9 (10%).

scholarly journals Distribution of emm type and antibiotic susceptibility of group A streptococci causing invasive and noninvasive disease

2008 ◽  
Vol 57 (11) ◽  
pp. 1383-1388 ◽  
Author(s):  
Takeaki Wajima ◽  
Somay Y. Murayama ◽  
Katsuhiko Sunaoshi ◽  
Eiichi Nakayama ◽  
Keisuke Sunakawa ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Macrolide Antibiotic ◽  
Group A Streptococcus ◽  
Acute Otitis Media ◽  
Toxic Shock ◽  
Medical Institutions ◽  
Invasive Infections ◽  
Group A ◽  
Resistant Strains ◽  
Emm Type

To determine the prevalence of macrolide antibiotic and levofloxacin resistance in infections with Streptococcus pyogenes (group A streptococcus or GAS), strains were collected from 45 medical institutions in various parts of Japan between October 2003 and September 2006. Four hundred and eighty-two strains from patients with GAS infections were characterized genetically. Strains were classified into four groups according to the type of infection: invasive infections (n=74) including sepsis, cellulitis and toxic-shock-like syndrome; acute otitis media (AOM; n=23); abscess (n=53); and pharyngotonsillitis (n=332). Among all strains, 32 emm types were identified; emm1 was significantly more common in invasive infections (39.2 %) and AOM (43.5 %) than in abscesses (3.8 %) or pharyngotonsillitis (10.2 %). emm12 and emm4 each accounted for 23.5 % of pharyngotonsillitis cases. Susceptibility of GAS strains to eight β-lactam agents was excellent, with MICs of 0.0005–0.063 μg ml−1. Macrolide-resistant strains accounted for 16.2 % of all strains, while the percentages of strains possessing the resistance genes erm(A), erm(B) and mef(A) were 2.5 %, 6.2 % and 7.5 %, respectively. Although no strains with high resistance to levofloxacin were found, strains with an MIC of 2–4 μg ml−1 (17.4 %) had amino acid substitutions at either Ser-79 or Asp-83 in ParC. These levofloxacin-intermediately resistant strains included 16 emm types, but macrolide-resistant strains were more likely than others to represent certain emm types.

Nonhemolytic Streptococcus pyogenes Causing Invasive Infection

2002 ◽  
Vol 41 (6) ◽  
pp. 453-453 ◽  
Author(s):  
N. Cimolai ◽  
C. Trombley ◽  
N. M. E Bhanju
Keyword(s):  
Streptococcus Pyogenes ◽  
Invasive Infection

scholarly journals Toxic shock syndrome related to Streptococcus equi subsp zooepidemicus

2013 ◽  
Vol 2013 (sep06 1) ◽  
pp. bcr2013200566-bcr2013200566 ◽  
Author(s):  
M. Saleh ◽  
V. Vialette
Keyword(s):  
Toxic Shock Syndrome ◽  
Toxic Shock ◽  
Streptococcus Equi
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