scholarly journals The Jekyll and Hyde Symbiont: Could Wolbachia Be a Nutritional Mutualist?

2019 ◽  
Vol 202 (4) ◽  
Author(s):  
Irene L. G. Newton ◽  
Danny W. Rice
Keyword(s):  
Large Scale ◽  
Gene Content ◽  
Scale Analysis ◽  
Content Type ◽  
Wolbachia Pipientis ◽  
Beneficial Role ◽  
Intracellular Symbiont ◽  
Large Scale Analysis ◽  
Conserved Gene

ABSTRACT The most common intracellular symbiont on the planet—Wolbachia pipientis—is infamous largely for the reproductive manipulations induced in its host. However, more recent evidence suggests that this bacterium may also serve as a nutritional mutualist in certain host backgrounds and for certain metabolites. We performed a large-scale analysis of conserved gene content across all sequenced Wolbachia genomes to infer potential nutrients made by these symbionts. We review and critically evaluate the prior research supporting a beneficial role for Wolbachia and suggest future experiments to test hypotheses of metabolic provisioning.

Readability of integrated reports: an exploratory global study

2019 ◽  
Vol 32 (5) ◽  
pp. 1532-1557
Author(s):  
Gerard William Stone ◽  
Sumit Lodhia
Keyword(s):  
Design Methodology ◽  
Large Scale ◽  
Scale Analysis ◽  
Integrated Reporting ◽  
Content Type ◽  
Corporate Reporting ◽  
Large Scale Analysis ◽  
Accessible Information ◽  
International Integrated Reporting Council ◽  
Practical Implications

Purpose A goal of integrated reporting (IR) under the International Integrated Reporting Council (IIRC)’s leadership is to provide clearly written, comprehensible and accessible information. In light of this objective, the purpose of this paper is to explore the readability and accessibility of integrated reports, an issue magnified by the IIRC’s continual commitment to clear and readable report language, and its intention for IR to become the corporate reporting norm. Design/methodology/approach In a whole text software facilitated analysis, the study utilises readability measures and supplementary measures of reader accessibility in a multi-year analysis of a large sample of global integrated reports sourced from the IIRC examples database. Findings The findings highlight the low readability of analysed integrated reports and indicate that readability is not improving. The supplementary measures suggest sub-optimal use of visual communication forms and overuse of structural presentation techniques which may contribute to reader accessibility of the analysed reports. Research limitations/implications The study extends readability analysis to an emerging corporate reporting phenomenon and its findings contribute to the growing IR literature. The study applies supplementary measures of reader accessibility which advance the methods available to assess the communication efficacy of integrated and other corporate reports. Practical implications The analysis of the readability and accessibility of integrated reports in the study indicates that the IIRC’s goal of clear, comprehensible and accessible reporting is not reflected by reporters’ practices. This has implications for the IIRC, reporting organisations, report readers and regulators. Originality/value The study represents the first large-scale analysis of the readability and accessibility of global integrated reports.

scholarly journals Simple Resazurin-Based Microplate Assay for Measuring Chlamydia Infections

2013 ◽  
Vol 57 (6) ◽  
pp. 2838-2840 ◽  
Author(s):  
Ichie Osaka ◽  
P. Scott Hefty
Keyword(s):  
Large Scale ◽  
Host Cells ◽  
Scale Analysis ◽  
Content Type ◽  
Large Scale Analysis ◽  
Resazurin Assay ◽  
Inhibitory Compound ◽  
Microscopy Method ◽  
Conventional Microscopy ◽  
Time Required

ABSTRACTThe conventional method for quantification ofChlamydiainfection using fluorescence microscopy typically involves time- and labor-intensive manual enumeration, which is not applicable for a large-scale analysis required for an inhibitory compound screen. In this study, an alamarBlue (resazurin) assay was adopted to measureChlamydiainfection by measuring the redox capability of infected host cells in a 96-well format. The assay provided measurements comparable to those of the conventional microscopy method while drastically reducing the time required for analysis.

scholarly journals Advances in decomposing complex metabolite mixtures using substructure- and network-based computational metabolomics approaches

2021 ◽  
Author(s):  
Mehdi A. Beniddir ◽  
Kyo Bin Kang ◽  
Grégory Genta-Jouve ◽  
Florian Huber ◽  
Simon Rogers ◽  
...  
Keyword(s):  
Natural Product ◽  
Large Scale ◽  
Scale Analysis ◽  
Computational Tools ◽  
Natural Product Discovery ◽  
Large Scale Analysis ◽  
Metabolite Annotation

This review highlights the key computational tools and emerging strategies for metabolite annotation, and discusses how these advances will enable integrated large-scale analysis to accelerate natural product discovery.

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