Simple Resazurin-Based Microplate Assay for Measuring Chlamydia Infections
2013 ◽
Vol 57
(6)
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pp. 2838-2840
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ABSTRACTThe conventional method for quantification ofChlamydiainfection using fluorescence microscopy typically involves time- and labor-intensive manual enumeration, which is not applicable for a large-scale analysis required for an inhibitory compound screen. In this study, an alamarBlue (resazurin) assay was adopted to measureChlamydiainfection by measuring the redox capability of infected host cells in a 96-well format. The assay provided measurements comparable to those of the conventional microscopy method while drastically reducing the time required for analysis.
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2019 ◽
Vol 32
(5)
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pp. 1532-1557
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