scholarly journals Mismatch correction in pneumococcal transformation: donor length and hex-dependent marker efficiency.

1976 ◽  
Vol 125 (1) ◽  
pp. 125-135 ◽  
Author(s):  
W R Guild ◽  
N B Shoemaker
Genetics ◽  
1996 ◽  
Vol 144 (2) ◽  
pp. 459-466 ◽  
Author(s):  
Yingying Yang ◽  
Anthony L Johnson ◽  
Leland H Johnston ◽  
Wolfram Siede ◽  
Errol C Friedberg ◽  
...  

Abstract RAD3 functions in DNA repair and transcription in Saccharomyces cerevisiae and particular rad3 alleles confer a mutator phenotype, possibly as a consequence of defective mismatch correction. We assessed the potential involvement of the Rad3 protein in mismatch correction by comparing heteroduplex repair in isogenic rad3-1 and wild-type strains. The rad3-1 allele increased the spontaneous mutation rate but did not prevent heteroduplex repair or bias its directionality. Instead, the efficiency of mismatch correction was enhanced in the rad3-1 strain. This surprising result prompted us to examine expression of yeast mismatch repair genes. We determined that MSH2, but not MLH1, is transcriptionally regulated during the cell-cycle like PMSl, and that rad3-1 does not increase the transcript levels for these genes in log phase cells. These observations suggest that the rad3-1 mutation gives rise to an enhanced efficiency of mismatch correction via a process that does not involve transcriptional regulation of mismatch repair. Interestingly, mismatch repair also was more efficient when error-editing by yeast DNA polymerase δ was eliminated. We discuss our results in relation to possible mechanisms that may link the rad3-1 mutation to mismatch correction efficiency.


2021 ◽  
Author(s):  
Simone Croci ◽  
Cagri Ozcinar ◽  
Emin Zerman ◽  
Roman Dudek ◽  
Sebastian Knorr ◽  
...  

ICTE 2015 ◽  
2015 ◽  
Author(s):  
Kun You ◽  
Haifeng Li ◽  
Wanqing Zhang ◽  
Sihan Yan

2011 ◽  
Vol 32 (2) ◽  
pp. 025008 ◽  
Author(s):  
Hongliang Zhao ◽  
Yiqiang Zhao ◽  
Junfeng Geng ◽  
Peng Li ◽  
Zhisheng Zhang

2020 ◽  
Vol 4 (3) ◽  
pp. 129-140
Author(s):  
A. A. Shevchuk ◽  
O. V. Pastushenko ◽  
V. V. Dvirniy ◽  
G. V. Dvirniy ◽  
A. A. Filatov

The reliability of both spacecraft as a whole and of their systems is confirmed at the stage of complex ground-based experimental tests, including complex thermal vacuum tests. The thermal state of the test object in thermal vacuum chambers is obtaining, in particular, using a solar simulator. Radiometers based on silicon photoelectric converters are most often used to control the irradiance of a solar simulator under conditions of thermal vacuum tests. At the same time, an analysis of the features of silicon photoelectric converters shows that their direct measurement with the accuracy required for ground-based tests of spacecraft is impossible; their output is nonlinear, depends on the received spectrum, their own temperature and has long-term instability. The achieved measurement accuracy directly depends on the number and accuracy of the tools used and the methods of the necessary correction, of which the mismatch correction between the solar simulator spectrum and the solar spectrum is the most difficult and laborious. At the same time, spectrally nonselective heat flux radiometers are free from the above disadvantages. In the course of the experiment we carried out, the significant dependence of the accuracy of measuring the irradiance with radiometers based on silicon photoelectric converters on the received spectrum was confirmed. The conclusion is made that direct measurement by heat flux radiometers of the irradiance of the solar simulator is most justified under the conditions of thermal vacuum tests.


2014 ◽  
Vol 49 (3) ◽  
pp. 608-621 ◽  
Author(s):  
Youngchang Yoon ◽  
Hyoungsoo Kim ◽  
Hyungwook Kim ◽  
Kun-Seok Lee ◽  
Chang-Ho Lee ◽  
...  

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