scholarly journals Use of temperature-sensitive mutants to study gene expression during sporulation in Bacillus subtilis.

1976 ◽  
Vol 126 (2) ◽  
pp. 928-936 ◽  
Author(s):  
M Young
Keyword(s):  
Gene Expression ◽  
Bacillus Subtilis ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants ◽  
Study Gene Expression

scholarly journals Role of SpoVA Proteins in Release of Dipicolinic Acid during Germination of Bacillus subtilis Spores Triggered by Dodecylamine or Lysozyme

2006 ◽  
Vol 189 (5) ◽  
pp. 1565-1572 ◽  
Author(s):  
Venkata Ramana Vepachedu ◽  
Peter Setlow
Keyword(s):  
Bacillus Subtilis ◽  
Small Molecules ◽  
Spore Germination ◽  
Dipicolinic Acid ◽  
Temperature Sensitive ◽  
Wild Type ◽  
Ph Optimum ◽  
Inner Membrane ◽  
Temperature Sensitive Mutants

ABSTRACT The release of dipicolinic acid (DPA) during the germination of Bacillus subtilis spores by the cationic surfactant dodecylamine exhibited a pH optimum of ∼9 and a temperature optimum of 60°C. DPA release during dodecylamine germination of B. subtilis spores with fourfold-elevated levels of the SpoVA proteins that have been suggested to be involved in the release of DPA during nutrient germination was about fourfold faster than DPA release during dodecylamine germination of wild-type spores and was inhibited by HgCl2. Spores carrying temperature-sensitive mutants in the spoVA operon were also temperature sensitive in DPA release during dodecylamine germination as well as in lysozyme germination of decoated spores. In addition to DPA, dodecylamine triggered the release of amounts of Ca2+ almost equivalent to those of DPA, and at least one other abundant spore small molecule, glutamic acid, was released in parallel with Ca2+ and DPA. These data indicate that (i) dodecylamine triggers spore germination by opening a channel in the inner membrane for Ca2+-DPA and other small molecules, (ii) this channel is composed at least in part of proteins, and (iii) SpoVA proteins are involved in the release of Ca2+-DPA and other small molecules during spore germination, perhaps by being a part of a channel in the spore's inner membrane.

scholarly journals Temperature-sensitive v-sea transformed erythroblasts: a model system to study gene expression during erythroid differentiation.

1988 ◽  
Vol 2 (2) ◽  
pp. 247-258 ◽  
Author(s):  
J Knight ◽  
M Zenke ◽  
C Disela ◽  
E Kowenz ◽  
P Vogt ◽  
...  
Keyword(s):  
Gene Expression ◽  
Erythroid Differentiation ◽  
Model System ◽  
Temperature Sensitive ◽  
Study Gene Expression

CONTROLLED GENE EXPRESSION IN BACILLUS SUBTILIS BASED ON THE TEMPERATURE-SENSITIVE λ cI REPRESSOR

1990 ◽  
pp. 3-12
Author(s):  
R. Breitling ◽  
A.V. Sorokin ◽  
Th. Ellinger ◽  
D. Behnke
Keyword(s):  
Gene Expression ◽  
Bacillus Subtilis ◽  
Temperature Sensitive

Temperature-sensitive mutants of Bacillus subtilis defective in spore outgrowth

1974 ◽  
Vol 131 (2) ◽  
pp. 147-157
Author(s):  
I. W. Dawes ◽  
H. O. Halvorson
Keyword(s):  
Bacillus Subtilis ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants ◽  
Spore Outgrowth

scholarly journals Temperature-Sensitive Mutants Affected in DNA Synthesis in Phage phi29 of Bacillus subtilis

1972 ◽  
Vol 31 (2) ◽  
pp. 367-371 ◽  
Author(s):  
Antonio Talavera ◽  
Margarita Salas ◽  
Eladio Vinuela
Keyword(s):  
Bacillus Subtilis ◽  
Dna Synthesis ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants
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