Asymmetric localization of lipopolysaccharides on the outer membrane of Salmonella typhimurium.

1980 ◽  
Vol 141 (3) ◽  
pp. 1463-1465 ◽  
Author(s):  
Y Funahara ◽  
H Nikaido
Keyword(s):  
Salmonella Typhimurium ◽  
Outer Membrane

​Optimization of Cultural Conditions for Maximum Production of Outer Membrane Vesicles from Salmonella Typhimurium

2021 ◽  
Author(s):  
Menguzotunuo Solo ◽  
Shantanu Tamuly ◽  
Luit Moni Barkalita ◽  
Girin Kumar Saikia ◽  
Dhruba Jyoti Kalita
Keyword(s):  
Oxidative Stress ◽  
Salmonella Typhimurium ◽  
Outer Membrane ◽  
Large Scale ◽  
Multiple Drug Resistance ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Multiple Drug ◽  
Scale Production ◽  
Maximum Production

Background: The non-typhoidal Salmonella causes gastroenteritis in humans that makes its way to the food chain mainly through the animal products. The multiple drug resistance imposes one of the major hurdle in the treatment of the disease. The vaccination appears to be the most important method for prevention of the disease. Unfortunately, there is no liscenced vaccine available against non-typhoidal Salmonellae. The use of outer membrane vesicles (OMVs) of Salmonella as a vaccine candidate has attained significant centre-stage in the recent years given to its protective immunogenicity. However, the large scale production of OMVs is difficult owing to low yield per liter of culture. Methods: In the present study, we have optimized the culture conditions viz. pH, phase of growth and presence of oxidative stress for maximum production of OMVs from Salmonella Typhimurium. The OMVs were characterized based on yield based on protein concentration, lipopolysaccharide concentration and zeta size. Result: In the present study, it was found that incubation of Salmonella Typhimurium up to peak of the growth phase at pH 7 in presence of oxidative stress was found to be the most suitable condition for maximum production of OMVs.

scholarly journals Topology of the outer membrane phospholipase A of Salmonella typhimurium.

1997 ◽  
Vol 179 (11) ◽  
pp. 3443-3450 ◽  
Author(s):  
K B Merck ◽  
H de Cock ◽  
H M Verheij ◽  
J Tommassen
Keyword(s):  
Salmonella Typhimurium ◽  
Outer Membrane ◽  
Phospholipase A ◽  
Outer Membrane Phospholipase A
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