Nucleotide sequence of the Salmonella typhimurium mutS gene required for mismatch repair: homology of MutS and HexA of Streptococcus pneumoniae.

Abstract To get more insight into the control of homologous recombination between diverged DNA by the Mut proteins of the long-patch mismatch repair system, we have studied interspecies Escherichia coli/Salmonella typhimurium recombination. Knowing that the same recombination pathway (RecABCD) is responsible for intraspecies and interspecies recombination, we have now studied the structure (replacement vs. addition-type or other rearrangement-type recombinants) of 81 interspecies recombinants obtained in conjugational crosses between E. coli donor and mutL, mutS, mutH, mutU or mut+ S. typhimurium recipients. Taking advantage of high interspecies sequence divergence, a physical analysis was performed on one third of the E. coli Hfr genome, which was expected to be transferred to S. typhimurium F- recipients during 40 min before interruption of the mating. Probes specific for each species were hybridized on dot blots of genomic DNA, or on colonies, and the composition of the rrn operons was determined from purified genomic DNA. With very few exceptions, the structure of these interspecies recombinants corresponds to replacements of one continuous block of the recipient genome by the corresponding region of the donor genome.

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Complete Genome Sequence of Streptococcus pneumoniae Strain Rx1, a Hex Mismatch Repair-Deficient Standard Transformation Recipient

Microbiology Resource Announcements ◽

10.1128/mra.00799-21 ◽

2021 ◽

Vol 10 (41) ◽

Author(s):

Anna Maria Cuppone ◽

Lorenzo Colombini ◽

Valeria Fox ◽

David Pinzauti ◽

Francesco Santoro ◽

...

Keyword(s):

Streptococcus Pneumoniae ◽

Mismatch Repair ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Gc Content ◽

Open Reading Frames ◽

Circular Chromosome ◽

Content Type ◽

Sequencing Technologies

The complete genome sequence of Streptococcus pneumoniae strain Rx1, a Hex mismatch repair-deficient standard transformation recipient, was obtained by combining Nanopore and Illumina sequencing technologies. The genome consists of a 2.03-Mb circular chromosome, with 2,054 open reading frames and a GC content of 39.72%.

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Directly repeated insertion of 9-nucleotide sequence detected in penicillin-binding protein 2B gene of penicillin-resistant Streptococcus pneumoniae.

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.40.5.1257 ◽

1996 ◽

Vol 40 (5) ◽

pp. 1257-1259 ◽

Cited By ~ 26

Author(s):

A Yamane ◽

H Nakano ◽

Y Asahi ◽

K Ubukata ◽

M Konno

Keyword(s):

Streptococcus Pneumoniae ◽

Nucleotide Sequence ◽

Molecular Mechanism ◽

Active Site ◽

Binding Protein ◽

Direct Repeat ◽

Penicillin Binding Protein ◽

Serine Residue ◽

Class A ◽

Class B

We investigated the molecular mechanism of 50 penicillin-resistant Streptococcus pneumoniae strains (penicillin: MIC, > or = 0.125 microgram/ml) having neither class A nor class B mutations in the penicillin-binding protein 2B gene (pbp2b). An analysis of the nucleotide sequences of the pbp2b genes from seven strains revealed an unique direct repeat of 9 nucleotides (TGGTATACT) between active-site serine (residue 385) and Ser-X-Asn (residues 442 to 444) motifs. The same insertion was detected in 13 strains.

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