scholarly journals Purification and partial characterization of the Rhizobium leguminosarum biovar viciae Ca2+-dependent adhesin, which mediates the first step in attachment of cells of the family Rhizobiaceae to plant root hair tips.

1989 ◽  
Vol 171 (7) ◽  
pp. 4054-4062 ◽  
Author(s):  
G Smit ◽  
T J Logman ◽  
M E Boerrigter ◽  
J W Kijne ◽  
B J Lugtenberg
2006 ◽  
Vol 87 (10) ◽  
pp. 3113-3117 ◽  
Author(s):  
Z. Kozlakidis ◽  
L. Covelli ◽  
F. Di Serio ◽  
A. Citir ◽  
S. Açıkgöz ◽  
...  

The sequence of the four large (L) double-stranded RNAs (dsRNAs) associated with Amasya cherry disease (ACD), which has a presumed fungal aetiology, is reported. ACD L dsRNAs 1 (5121 bp) and 2 (5047 bp) potentially encode proteins of 1628 and 1620 aa, respectively, that are 37 % identical and of unknown function. ACD L dsRNAs 3 (4458 bp) and 4 (4303 bp) potentially encode proteins that are 68 % identical and contain the eight motifs conserved in RNA-dependent RNA polymerases (RdRp) of dsRNA mycoviruses, having highest similarity with those of members of the family Totiviridae. Both terminal regions share extensive conservation in all four RNAs, suggesting a functional relationship between them. As ACD L dsRNAs 1 and 2 do not encode RdRps, both are probably replicated by those from either ACD L dsRNA 3 or 4. Partial characterization of the equivalent L dsRNAs 3 and 4 associated with cherry chlorotic rusty spot revealed essentially identical sequences.


1987 ◽  
Vol 84 (2) ◽  
pp. 421-427 ◽  
Author(s):  
Russell W. Carlson ◽  
Robert Shatters ◽  
Jauh-Lin Duh ◽  
Elroy Turnbull ◽  
Brian Hanley ◽  
...  

Author(s):  
Dániel Garamvölgyi ◽  
Tibor Jordán

AbstractA d-dimensional framework is a pair (G, p), where $$G=(V,E)$$ G = ( V , E ) is a graph and p is a map from V to $$\mathbb {R}^d$$ R d . The length of an edge $$uv\in E$$ u v ∈ E in (G, p) is the distance between p(u) and p(v). The framework is said to be globally rigid in $$\mathbb {R}^d$$ R d if every other d-dimensional framework (G, q), in which the corresponding edge lengths are the same, is congruent to (G, p). In a recent paper Gortler, Theran, and Thurston proved that if every generic framework (G, p) in $$\mathbb {R}^d$$ R d is globally rigid for some graph G on $$n\ge d+2$$ n ≥ d + 2 vertices (where $$d\ge 2$$ d ≥ 2 ), then already the set of (unlabeled) edge lengths of a generic framework (G, p), together with n, determine the framework up to congruence. In this paper we investigate the corresponding unlabeled reconstruction problem in the case when the above generic global rigidity property does not hold for the graph. We provide families of graphs G for which the set of (unlabeled) edge lengths of any generic framework (G, p) in d-space, along with the number of vertices, uniquely determine the graph, up to isomorphism. We call these graphs weakly reconstructible. We also introduce the concept of strong reconstructibility; in this case the labeling of the edges is also determined by the set of edge lengths of any generic framework. For $$d=1,2$$ d = 1 , 2 we give a partial characterization of weak reconstructibility as well as a complete characterization of strong reconstructibility of graphs. In particular, in the low-dimensional cases we describe the family of weakly reconstructible graphs that are rigid but not redundantly rigid.


1994 ◽  
Vol 21 (3) ◽  
pp. 311 ◽  
Author(s):  
J Plazinski ◽  
RW Ridge ◽  
IA Mckay ◽  
MA Djordjevic

Cloned DNA fragments coding for the nodDABC genes of Rhizobium leguminosarum biovar trifolii strain ANU843 were introduced into Rhizobium strains possessing Sym plasmid deletions. These strains were able to: (a) synthesise four butanol-soluble Nod metabolites; (b) affect the normal growth pattern of plant root hairs of a wide range of host and non-host legumes; and (c) induce many root outgrowths on Phaseolus plants. The four Nod metabolites produced by these strains were labelled by supplying cultures with 14C-acetate in the presence of a flavonoid inducer of nod gene expression. In contrast, more than ten Nod metabolites were synthesised by wild-type strains or constructed strains containing the full complement of R. leguminosarum biovar. trifolii nodulation and host specific nodulation genes. Strain ANU845 containing nodDABC did not induce infection threads or nodule initiation sites but distorted and curled cells in plant root hairs. However strain ANU845 induced root outgrowths on beans (Phaseolus vulgaris) that appeared to result from a proliferation of the epidermal tissue. Transfer of plasmids bearing nodDABC to various Gram-negative bacteria, Agrobacterium tumefaciens, Pseudomonas aeruginosa, Lignobacter sp., Azospirillum brasilense and Escherichia coli, and different non-nodulating mutant rhizobia conferred on these strains the ability to cause root-hair curling and distortions. Several strains induced root-hair curling on clover and a range of other non-host legumes. We suggest that the expression of nodDABC in a range of soil bacteria may extend or alter the effects of these soil bacteria on the roots of host plants.


Author(s):  
Antonín Stratil ◽  
Petr Bobj'ak ◽  
Miloslav Valenta ◽  
Vladimír Tomášek

1979 ◽  
Author(s):  
M Ribieto ◽  
J Elion ◽  
D Labie ◽  
F Josso

For the purification of the abnormal prothrombin (Pt Metz), advantage has been taken of the existence in the family of three siblings who, being double heterozygotes for Pt Metz and a hypoprothrombinemia, have no normal Pt. Purification procedures included barium citrate adsorption and chromatography on DEAE Sephadex as for normal Pt. As opposed to some other variants (Pt Barcelona and Madrid), Pt Metz elutes as a single symetrical peak. By SDS polyacrylamide gel electrophoresis, this material is homogeneous and appears to have the same molecular weight as normal Pt. Comigration of normal and abnormal Pt in the absence of SDS, shows a double band suggesting an abnormal charge for the variant. Pt Metz exhibits an identity reaction with the control by double immunodiffusion. Upon activation by factor Xa, Pt Metz can generate amydolytic activity on Bz-Phe-Val-Arg-pNa (S2160), but only a very low clotting activity. Clear abnormalities are observed in the cleavage pattern of Pt Metz when monitored by SDS gel electrophoresis. The main feature are the accumulation of prethrombin l (Pl) and the appearance of abnormal intermediates migrating faster than Pl.


2010 ◽  
Vol 108 (10) ◽  
pp. 323-329 ◽  
Author(s):  
Marti F. A. Bierhuizen ◽  
Moniek de Wit ◽  
Carin A. R. L. Govers ◽  
Willem van Dijk

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