scholarly journals How the phage lambda N gene product suppresses transcription termination: communication of RNA polymerase with regulatory proteins mediated by signals in nascent RNA.

1992 ◽  
Vol 174 (21) ◽  
pp. 6711-6716 ◽  
Author(s):  
A Das
Keyword(s):  
Rna Polymerase ◽  
Gene Product ◽  
Transcription Termination ◽  
Regulatory Proteins ◽  
N Gene ◽  
Phage Lambda ◽  
Nascent Rna

Genetic analysis of the N transcription antitermination system of phage λ

Genome ◽  
1989 ◽  
Vol 31 (2) ◽  
pp. 491-496 ◽  
Author(s):  
David I. Friedman ◽  
Andrew E. Granston ◽  
Debra Thompson ◽  
Alan T. Schauer ◽  
Eric R. Olson
Keyword(s):  
Gene Expression ◽  
Genetic Analysis ◽  
Rna Polymerase ◽  
Gene Product ◽  
Transcription Termination ◽  
N Gene ◽  
Encoded Proteins ◽  
Transcription Antitermination

Regulation of coliphage λ gene expression occurs, in part, by a process of termination and antitermination of transcription. The N gene product, one of two λ encoded antitermination proteins, acts with host encoded proteins, Nus, at sites, nut, located downstream of the early promoters to render the transcribing RNA polymerase resistant to many downstream termination signals. We discuss the nature of some of the proteins and sites involved in this process.Key words: regulation, transcription, termination, antitermination, nusA, N, boxA, nut.

Transcription termination by RNA polymerase III: uncoupling of polymerase release from termination signal recognition

1992 ◽  
Vol 12 (5) ◽  
pp. 2260-2272
Author(s):  
F E Campbell ◽  
D R Setzer
Keyword(s):  
Rna Polymerase ◽  
Transcription Termination ◽  
Transcription Elongation ◽  
Rna Polymerase Iii ◽  
5S Rrna ◽  
Rrna Gene ◽  
Signal Recognition ◽  
Polymerase Iii ◽  
5S Rrna Gene ◽  
Nascent Rna

Xenopus RNA polymerase III specifically initiates transcription on poly(dC)-tailed DNA templates in the absence of other class III transcription factors normally required for transcription initiation. In experimental analyses of transcription termination using DNA fragments with a 5S rRNA gene positioned downstream of the tailed end, only 40% of the transcribing polymerase molecules terminate at the normally efficient Xenopus borealis somatic-type 5S rRNA terminators; the remaining 60% read through these signals and give rise to runoff transcripts. We find that the nascent RNA strand is inefficiently displaced from the DNA template during transcription elongation. Interestingly, only polymerases synthesizing a displaced RNA terminate at the 5S rRNA gene terminators; when the nascent RNA is not displaced from the template, read-through transcripts are synthesized. RNAs with 3' ends at the 5S rRNA gene terminators are judged to result from authentic termination events on the basis of multiple criteria, including kinetic properties, the precise 3' ends generated, release of transcripts from the template, and recycling of the polymerase. Even though only 40% of the polymerase molecules ultimately terminate at either of the tandem 5S rRNA gene terminators, virtually all polymerases pause there, demonstrating that termination signal recognition can be experimentally uncoupled from polymerase release. Thus, termination is dependent on RNA strand displacement during transcription elongation, whereas termination signal recognition is not. We interpret our results in terms of a two-step model for transcription termination in which polymerase release is dependent on the fate of the nascent RNA strand during transcription elongation.

Regulation of transcription termination in Escherichia coli

1984 ◽  
Vol 62 (2-3) ◽  
pp. 79-88 ◽  
Author(s):  
Jack Greenblatt
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Transcription Termination ◽  
Mechanisms Of Action ◽  
Chain Elongation ◽  
Current Evidence ◽  
N Gene ◽  
Regulation Of Transcription ◽  
Bacterial Transcription ◽  
Transcriptional Termination

Experiments are summarized that deal with the mechanisms of action of the N gene transcriptional antitermination protein of bacteriophage lambda and the nusA gene transcriptional termination protein of Escherichia coli. Models are presented for the regulation of chain elongation during bacterial transcription. Current evidence suggests that chain elongation during transcription may be regulated by a "regulosome" that consists of RNA and protein and is bound to transcribing RNA polymerase.

scholarly journals The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Sina Wittmann ◽  
Max Renner ◽  
Beth R. Watts ◽  
Oliver Adams ◽  
Miles Huseyin ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcription Termination ◽  
Nascent Rna

scholarly journals Transcription termination by RNA polymerase III: uncoupling of polymerase release from termination signal recognition.

1992 ◽  
Vol 12 (5) ◽  
pp. 2260-2272 ◽  
Author(s):  
F E Campbell ◽  
D R Setzer
Keyword(s):  
Rna Polymerase ◽  
Transcription Termination ◽  
Transcription Elongation ◽  
Rna Polymerase Iii ◽  
5S Rrna ◽  
Rrna Gene ◽  
Signal Recognition ◽  
Polymerase Iii ◽  
5S Rrna Gene ◽  
Nascent Rna

Xenopus RNA polymerase III specifically initiates transcription on poly(dC)-tailed DNA templates in the absence of other class III transcription factors normally required for transcription initiation. In experimental analyses of transcription termination using DNA fragments with a 5S rRNA gene positioned downstream of the tailed end, only 40% of the transcribing polymerase molecules terminate at the normally efficient Xenopus borealis somatic-type 5S rRNA terminators; the remaining 60% read through these signals and give rise to runoff transcripts. We find that the nascent RNA strand is inefficiently displaced from the DNA template during transcription elongation. Interestingly, only polymerases synthesizing a displaced RNA terminate at the 5S rRNA gene terminators; when the nascent RNA is not displaced from the template, read-through transcripts are synthesized. RNAs with 3' ends at the 5S rRNA gene terminators are judged to result from authentic termination events on the basis of multiple criteria, including kinetic properties, the precise 3' ends generated, release of transcripts from the template, and recycling of the polymerase. Even though only 40% of the polymerase molecules ultimately terminate at either of the tandem 5S rRNA gene terminators, virtually all polymerases pause there, demonstrating that termination signal recognition can be experimentally uncoupled from polymerase release. Thus, termination is dependent on RNA strand displacement during transcription elongation, whereas termination signal recognition is not. We interpret our results in terms of a two-step model for transcription termination in which polymerase release is dependent on the fate of the nascent RNA strand during transcription elongation.

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