scholarly journals Similarity between the Myxococcus xanthus and Stigmatella aurantiaca reverse transcriptase genes associated with multicopy, single-stranded DNA.

1992 ◽  
Vol 174 (7) ◽  
pp. 2384-2387 ◽  
Author(s):  
M Y Hsu ◽  
C Xu ◽  
M Inouye ◽  
S Inouye
Keyword(s):  
Reverse Transcriptase ◽  
Myxococcus Xanthus ◽  
Single Stranded Dna ◽  
Stigmatella Aurantiaca

Reverse transcriptase associated with the biosynthesis of the branched RNA-linked msDNA in Myxococcus xanthus

Cell ◽  
1989 ◽  
Vol 56 (4) ◽  
pp. 709-717 ◽  
Author(s):  
Sumiko Inouye ◽  
Mei-Yin Hsu ◽  
Susan Eagle ◽  
Masayori Inouye
Keyword(s):  
Reverse Transcriptase ◽  
Myxococcus Xanthus ◽  
Branched Rna

Branched RNA covalently linked to the 5′ end of a single-stranded DNA in Stigmatella aurantiaca: Structure of msDNA

Cell ◽  
1987 ◽  
Vol 48 (1) ◽  
pp. 47-53 ◽  
Author(s):  
Teiichi Furuichi ◽  
Anil Dhundale ◽  
Masayori Inouye ◽  
Sumiko Inouye
Keyword(s):  
Single Stranded Dna ◽  
Stigmatella Aurantiaca ◽  
Branched Rna ◽  
Covalently Linked

scholarly journals A reverse transcriptase-like activity of wheat (Triticum aestivum) embryo microsomal fraction

1987 ◽  
Vol 248 (1) ◽  
pp. 309-312 ◽  
Author(s):  
E Kraszewska ◽  
B Marciniak ◽  
J Buchowicz
Keyword(s):  
Triticum Aestivum ◽  
Reverse Transcriptase ◽  
Alkaline Hydrolysis ◽  
Microsomal Fraction ◽  
Maximal Activity ◽  
Incubation Mixture ◽  
Single Stranded Dna ◽  
Wheat Embryo ◽  
Polyadenylated Rna ◽  
Embryo Cells

A reverse transcriptase-like activity was isolated from germinating wheat (Triticum aestivum) embryos. The activity was found to be associated with a microsomal fraction (70,000 g pellet) of the embryo homogenate. The microsome-associated enzyme prefers homologous polyadenylated RNA to any other polynucleotides as template and requires all four deoxyribonucleoside triphosphates for maximal activity. The reaction product appears in the incubation mixture in the form of an RNA-DNA hybrid, which can be converted into single-stranded DNA by mild alkaline hydrolysis. These observations suggest that normal wheat embryo cells contain an enzyme which, functionally, is similar to retroviral reverse transcriptase.

Multicopy single-stranded DNA isolated from a gram-negative bacterium, Myxococcus xanthus

Cell ◽  
1984 ◽  
Vol 38 (1) ◽  
pp. 203-209 ◽  
Author(s):  
Thomas Yee ◽  
Teiichi Furuichi ◽  
Sumiko Inouye ◽  
Masayori Inouye
Keyword(s):  
Myxococcus Xanthus ◽  
Negative Bacterium ◽  
Single Stranded Dna ◽  
Gram Negative

scholarly journals Bacterial retrons encode tripartite toxin/antitoxin systems

2020 ◽  
Author(s):  
Jacob Bobonis ◽  
André Mateus ◽  
Birgit Pfalz ◽  
Sarela Garcia-Santamarina ◽  
Marco Galardini ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Genome Editing ◽  
Physiological Role ◽  
Bacterial Genomes ◽  
Single Stranded Dna ◽  
Ambient Temperatures ◽  
E Coli

ABSTRACTRetrons are genetic retroelements, commonly found in bacterial genomes and recently repurposed as genome editing tools. Their encoded reverse transcriptase (RT) produces a multi-copy single-stranded DNA (msDNA). Despite our understanding of their complex biosynthesis, the function of msDNAs and therefore, the physiological role of retrons has remained elusive. We establish that the retron-Sen2 in Salmonella Typhimurium encodes a toxin, which we have renamed as RcaT (Retron cold-anaerobic Toxin). RcaT is activated when msDNA biosynthesis is perturbed and its toxicity is higher at ambient temperatures or during anaerobiosis. The RT and msDNA form together the antitoxin unit, with the RT binding RcaT, and the msDNA enabling the antitoxin activity. Using another E. coli retron, we establish that this toxin/antitoxin function is conserved, and that RT-toxin interactions are cognate. Altogether, retrons constitute a novel family of tripartite toxin/antitoxin systems.

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