scholarly journals Genetic analysis of the virD operon of Agrobacterium tumefaciens: a search for functions involved in transport of T-DNA into the plant cell nucleus and in T-DNA integration.

1993 ◽  
Vol 175 (3) ◽  
pp. 723-731 ◽  
Author(s):  
Z Koukolíková-Nicola ◽  
D Raineri ◽  
K Stephens ◽  
C Ramos ◽  
B Tinland ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Analysis ◽  
Plant Cell ◽  
Cell Nucleus ◽  
Dna Integration

T-DNA integration patterns in transgenic plants mediated by Agrobacterium tumefaciens

2011 ◽  
Vol 33 (12) ◽  
pp. 1327-1334 ◽  
Author(s):  
Lin YANG ◽  
Feng-Ling FU ◽  
Wan-Chen LI
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transgenic Plants ◽  
Dna Integration

Genetic analysis of mannityl opine catabolism in octopine-type Agrobacterium tumefaciens strain 15955

1987 ◽  
Vol 208 (1-2) ◽  
pp. 301-308 ◽  
Author(s):  
Yves Dessaux ◽  
Jacques Tempé ◽  
Stephen K. Farrand
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Analysis

scholarly journals Agrobacterium tumefaciens Transformation of the Radiation Hypersensitive Arabidopsis thaliana Mutants uvh1 and rad5

1998 ◽  
Vol 11 (11) ◽  
pp. 1136-1141 ◽  
Author(s):  
Jaesung Nam ◽  
Kirankumar S. Mysore ◽  
Stanton B. Gelvin
Keyword(s):  
Arabidopsis Thaliana ◽  
Agrobacterium Tumefaciens ◽  
Transformation Process ◽  
Stable Transformation ◽  
Wild Type ◽  
Dna Integration ◽  
Agrobacterium Tumefaciens Transformation ◽  
Inoculation Assay

The Arabidopsis thaliana mutants uvh1 and rad5, originally identified as radiation hypersensitive, were reported to be deficient in T-DNA integration based on the relative efficiencies of stable transformation and T-DNA transfer. We reassessed these mutants for susceptibility to transformation by Agrobacterium tumefaciens. The mutant rad5 showed a significant reduction in the efficiency of transient as well as stable transformation, compared with its wild-type progenitor. These data indicate that rad5 is blocked at a step in the transformation process prior to T-DNA integration. We additionally found, using both an in vitro root inoculation and an in vivo flower bolt inoculation assay, that the mutant uvh1 is as susceptible to A. tumefaciens-mediated transformation as is its wild-type progenitor, C10.

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