scholarly journals Role of the acrAB locus in organic solvent tolerance mediated by expression of marA, soxS, or robA in Escherichia coli.

1997 ◽  
Vol 179 (19) ◽  
pp. 6122-6126 ◽  
Author(s):  
D G White ◽  
J D Goldman ◽  
B Demple ◽  
S B Levy
Keyword(s):  
Escherichia Coli ◽  
Organic Solvent ◽  
Solvent Tolerance ◽  
Organic Solvent Tolerance

scholarly journals Role of the Multidrug Efflux Systems ofPseudomonas aeruginosa in Organic Solvent Tolerance

1998 ◽  
Vol 180 (11) ◽  
pp. 2987-2991 ◽  
Author(s):  
Xian-Zhi Li ◽  
Li Zhang ◽  
Keith Poole
Keyword(s):  
Organic Solvent ◽  
Organic Solvents ◽  
Efflux Pump ◽  
Solvent Tolerance ◽  
Multidrug Efflux ◽  
Efflux System ◽  
Organic Solvent Tolerance ◽  
Multidrug Efflux Pumps ◽  
Energy Dependent

ABSTRACT Multidrug efflux pumps with a broad substrate specificity make a major contribution to intrinsic and acquired multiple antibiotic resistance in Pseudomonas aeruginosa. Using genetically defined efflux pump mutants, we investigated the involvement of the three known efflux systems, MexA-MexB-OprM, MexC-MexD-OprJ, and MexE-MexF-OprN, in organic solvent tolerance in this organism. Our results showed that all three systems are capable of providing some level of tolerance to organic solvents such as n-hexane andp-xylene. Expression of MexAB-OprM was correlated with the highest levels of tolerance, and indeed, this efflux system was a major contributor to the intrinsic solvent tolerance of P. aeruginosa. Intrinsic organic solvent tolerance was compromised by a protonophore, indicating that it is substantially energy dependent. These data suggest that the efflux of organic solvents is a factor in the tolerance of P. aeruginosa to these compounds and that the multidrug efflux systems of this organism can accommodate organic solvents, as well as antibiotics.

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