scholarly journals Native Plasmids of Fusobacterium nucleatum: Characterization and Use in Development of Genetic Systems

2000 ◽  
Vol 182 (4) ◽  
pp. 1176-1180 ◽  
Author(s):  
Susan Kinder Haake ◽  
Sean C. Yoder ◽  
Gwynne Attarian ◽  
Kara Podkaminer
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Dna Sequence ◽  
Fusobacterium Nucleatum ◽  
Dna Sequence Analysis ◽  
Modification System ◽  
Restriction Modification System ◽  
Shuttle Plasmid ◽  
Genetic Systems ◽  
Restriction Modification

ABSTRACT Three native plasmids of Fusobacterium nucleatum were characterized, including DNA sequence analysis of one plasmid, pFN1. A shuttle plasmid, pHS17, capable of transforming Escherichia coli and F. nucleatum ATCC 10953 was constructed with pFN1. pHS17 was stably maintained in the F. nucleatum transformants, and differences in the transformation efficiencies suggested the presence of a restriction-modification system in F. nucleatum.

Cloning the KpnI restriction-modification system in Escherichia coli

Gene ◽  
1991 ◽  
Vol 97 (1) ◽  
pp. 97-102 ◽  
Author(s):  
Alan W. Hammond ◽  
Gary F. Gerard ◽  
Deb K. Chatterjee
Keyword(s):  
Escherichia Coli ◽  
Modification System ◽  
Restriction Modification System ◽  
Restriction Modification

scholarly journals The EcoKI Type I Restriction-Modification System in Escherichia coli Affects but Is Not an Absolute Barrier for Conjugation

2014 ◽  
Vol 197 (2) ◽  
pp. 337-342 ◽  
Author(s):  
Louise Roer ◽  
Frank M. Aarestrup ◽  
Henrik Hasman
Keyword(s):  
Escherichia Coli ◽  
Rapid Evolution ◽  
Type I ◽  
Modification System ◽  
Major Barrier ◽  
Exogenous Dna ◽  
Content Type ◽  
Restriction Modification System ◽  
K 12 ◽  
Restriction Modification

The rapid evolution of bacteria is crucial to their survival and is caused by exchange, transfer, and uptake of DNA, among other things. Conjugation is one of the main mechanisms by which bacteria share their DNA, and it is thought to be controlled by varied bacterial immune systems. Contradictory results about restriction-modification systems based on phenotypic studies have been presented as reasons for a barrier to conjugation with and other means of uptake of exogenous DNA. In this study, we show that inactivation of the R.EcoKI restriction enzyme in strainEscherichia coliK-12 strain MG1655 increases the conjugational transfer of plasmid pOLA52, which carriers two EcoKI recognition sites. Interestingly, the results were not absolute, and uptake of unmethylated pOLA52 was still observed in the wild-type strain (with an intacthsdRgene) but at a reduction of 85% compared to the uptake of the mutant recipient with a disruptedhsdRgene. This leads to the conclusion that EcoKI restriction-modification affects the uptake of DNA by conjugation but is not a major barrier to plasmid transfer.

Genetic mapping and DNA sequence analysis of mutations in the polA gene of Escherichia coli

1985 ◽  
Vol 186 (2) ◽  
pp. 283-293 ◽  
Author(s):  
Catherine M. Joyce ◽  
David M. Fujii ◽  
Helaina S. Laks ◽  
Cynthia M. Hughes ◽  
Nigel D.F. Grindley
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Genetic Mapping ◽  
Dna Sequence ◽  
Dna Sequence Analysis
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