Characterization of Two Methanopterin Biosynthesis Mutants of Methylobacterium extorquens AM1 by Use of a Tetrahydromethanopterin Bioassay
2004 ◽
Vol 186
(5)
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pp. 1565-1570
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Keyword(s):
ABSTRACT An enzymatic assay was developed to measure tetrahydromethanopterin (H4MPT) levels in wild-type and mutant cells of Methylobacterium extorquens AM1. H4MPT was detectable in wild-type cells but not in strains with a mutation of either the orf4 or the dmrA gene, suggesting a role for these two genes in H4MPT biosynthesis. The protein encoded by orf4 catalyzed the reaction of ribofuranosylaminobenzene 5′-phosphate synthase, the first committed step of H4MPT biosynthesis. These results provide the first biochemical evidence for H4MPT biosynthesis genes in bacteria.
2010 ◽
Vol 75
(12)
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pp. 1435-1443
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Keyword(s):
2003 ◽
Vol 69
(12)
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pp. 7563-7566
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1984 ◽
Vol 4
(11)
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pp. 2479-2485
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Keyword(s):
1995 ◽
Vol 108
(5)
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pp. 2065-2076
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Keyword(s):
1990 ◽
Vol 136
(1)
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pp. 181-188
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1991 ◽
Vol 137
(4)
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pp. 895-904
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1984 ◽
Vol 4
(11)
◽
pp. 2479-2485
Keyword(s):
1991 ◽
Vol 173
(22)
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pp. 7228-7232
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