scholarly journals Interactions Between Macrophages of Guinea Pigs and Salmonellae I. Fate of Salmonella typhimurium Within Macrophages of Normal Guinea Pigs1

1968 ◽  
Vol 96 (1) ◽  
pp. 191-197 ◽  
Author(s):  
H. S. Hsu ◽  
Anna S. Radcliffe
Keyword(s):  
Salmonella Typhimurium ◽  
Guinea Pigs

scholarly journals The immunity produced by a rough Salmonella dublin variant against Salmonella typhimurium and Salmonella choleraesuis infection in guinea-pigs

1966 ◽  
Vol 64 (3) ◽  
pp. 357-359 ◽  
Author(s):  
H. Williams Smith ◽  
Sheila Halls
Keyword(s):  
Salmonella Typhimurium ◽  
Guinea Pigs ◽  
Harmful Effect ◽  
Salmonella Dublin ◽  
Salmonella Choleraesuis

Vaccination with a rough variant of Salmonella dublin had no observable harmful effect on guinea-pigs. It conferred a good immunity against S. typhimurium and S. choleraesuis var kunzendorf infections.

scholarly journals Vaccination of pregnant guinea pigs with aromatic dependent Salmonella typhimurium to protect their newborns

2006 ◽  
Vol 30 (1) ◽  
pp. 100-110
Author(s):  
Yousif A. A.
Keyword(s):  
Salmonella Typhimurium ◽  
Skin Test ◽  
Guinea Pigs ◽  
Agglutination Test ◽  
Typhimurium Strain ◽  
Group 6 ◽  
Group 15

Genetically altered stable non reverting aromatic dependent (aro)Salmonella typhimurium,strain SL1479 was administrated intramuscularly tohealthy pregnant guinea pigs as alive vaccine. Twenty one pregnant guinea pigswere divided into two groups, the first group (15 animals) was vaccinated twicewith 1ml containing 17 C.f.U /ml approximately fourth and second week preparturitionand the second group (6 animals) injected with 1ml trypticase soybroth (TSB) as a control group.Adverse reaction to vaccination were not observed in the pregnant guineapigs, which parturated normally. The vaccine induced humoral and cellularimmune response as measured by tube agglutination test and delayed typehypersensitivity(DTH)-skin test in the immunized dams and transfer of thisresponse to the newborns, which revealed a high titers ofO(somatic)&H(flagller) agglutination titers and positive delayed typehypersensitivity(DTH)- skin test.The newborn overcome the challenge with virulent Salmonellatyphimurium at 3,6 &8 weeks of age, compared with the controlnewborn which died. These results revealed the efficacy of theprenatal vaccination with aro Salmonella typhimurium to transfer thepassive immunity to the newborn.

scholarly journals Epizootiological Studies of Salmonella typhimurium Infection in Guinea Pigs

1987 ◽  
Vol 36 (1) ◽  
pp. 39-49
Author(s):  
Osamu T. IIJIMA ◽  
Manabu SAITO ◽  
Kazue NAKAYAMA ◽  
Shinobu KOBAYASHI ◽  
Kumiko MATSUNO ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Guinea Pigs

scholarly journals Protective activity of specific transfer factor against Salmonella typhimurium infection

2021 ◽  
Vol 25 (1) ◽  
pp. 143-152
Author(s):  
A. A. Yousif ◽  
K. A. Khalifa ◽  
S. A.G. Al-Samarrae
Keyword(s):  
Salmonella Typhimurium ◽  
Skin Test ◽  
Transfer Factor ◽  
Guinea Pigs ◽  
Delayed Type Hypersensitivity ◽  
Cell Mediated Immunity ◽  
Recipient Group ◽  
Recipient Animal ◽  
Hypersensitivity Skin

Specific transfer factor (TF) extracted from spleens of sensitized and non-sensitized guinea pigs to study the efficacy of transfer of cellular immunity specific for salmonella . Two groups each of five guinea pigs were used for in vivo TF preparation. The First group was inoculated with 1 ml of aromatic dependent Salmonella typhimurium SL 1479 vaccine at a dose of 10' cfu/ml intramuscularly twice at two weeks intervals. The second group was injected with trypticase soy broth similarly . These two groups used as a donor for TF, and TFn respectively. Twenty one recipient guinea pigs were divided into three groups, the first group was TF at a dose of 1 ml equivalent to 5X10 cell intramuscularly three times/2 days intervals, Similarly the second group was given TF, where as the third group was given PBS- Cell mediated immunity in recipient animal was evaluated by delayed type hypersensitivity - skin test, Macrophage migration inhibition test (MIF) and then challenged with virulent Salmonella typhimurium. The TFt recipient group induced skin test and showed migration indices less than 0.8 and overcome the challenge organism. Contrary to TFn & PBS recipient groups which did not show any response for skin test and given migration indices more than 0.8 and did not show resistance for virulent Salmonella typhimurium .

Investigation of the relaxant effects of propofol on ovalbumin-induced asthma in guinea pigs

2006 ◽  
pp. 1
Author(s):  
I. Bagcivan ◽  
O. Cevit ◽  
M. K. Yildirim ◽  
S. Gursoy ◽  
S. Yildirim ◽  
...  
Keyword(s):  
Guinea Pigs

Hepatocyte Alterations in Guinea Pigs FED Polychlorinated Biphenyls (PCBs), Dibenzodioxins (PCDD), AND DIBENZOFURANS (PCDF)

1982 ◽  
Vol 40 ◽  
pp. 56-57
Author(s):  
J. N. Turner ◽  
D. N. Collins
Keyword(s):  
Guinea Pigs ◽  
Room Temperature ◽  
Dose Group ◽  
Methyl Cellulose ◽  
Uranyl Acetate ◽  
Target Organ ◽  
Office Building ◽  
Lead Citrate ◽  
Control Groups ◽  
Cooling Fluid

A fire involving an electric service transformer and its cooling fluid, a mixture of PCBs and chlorinated benzenes, contaminated an office building with a fine soot. Chemical analysis showed PCDDs and PCDFs including the highly toxic tetra isomers. Guinea pigs were chosen as an experimental animal to test the soot's toxicity because of their sensitivity to these compounds, and the liver was examined because it is a target organ. The soot was suspended in 0.75% methyl cellulose and administered in a single dose by gavage at levels of 1,10,100, and 500mgm soot/kgm body weight. Each dose group was composed of 6 males and 6 females. Control groups included 12 (6 male, 6 female) animals fed activated carbon in methyl cellulose, 6 males fed methyl cellulose, and 16 males and 10 females untreated. The guinea pigs were sacrificed at 42 days by suffocation in CO2. Liver samples were immediately immersed and minced in 2% gluteraldehyde in cacadylate buffer at pH 7.4 and 4°C. After overnight fixation, samples were postfixed in 1% OsO4 in cacodylate for 1 hr at room temperature, embedded in epon, sectioned and stained with uranyl acetate and lead citrate.

Glycogen in guinea pig neutrophils: Ultrastructural study of neutrophils at the intradermal site of BCG injection

1983 ◽  
Vol 41 ◽  
pp. 726-727
Author(s):  
Corazon D. Bucana
Keyword(s):  
Bone Marrow ◽  
Guinea Pig ◽  
Electron Density ◽  
Peritoneal Cavity ◽  
Ultrastructural Study ◽  
Guinea Pigs ◽  
Random Distribution ◽  
Glycogen Content ◽  
Polymorphonuclear Neutrophils ◽  
Ultrastructural Studies

In the circulating blood of man and guinea pigs, glycogen occurs primarily in polymorphonuclear neutrophils and platelets. The amount of glycogen in neutrophils increases with time after the cells leave the bone marrow, and the distribution of glycogen in neutrophils changes from an apparently random distribution to large clumps when these cells move out of the circulation to the site of inflammation in the peritoneal cavity. The objective of this study was to further investigate changes in glycogen content and distribution in neutrophils. I chose an intradermal site because it allows study of neutrophils at various stages of extravasation.Initially, osmium ferrocyanide and osmium ferricyanide were used to fix glycogen in the neutrophils for ultrastructural studies. My findings confirmed previous reports that showed that glycogen is well preserved by both these fixatives and that osmium ferricyanide protects glycogen from solubilization by uranyl acetate.I found that osmium ferrocyanide similarly protected glycogen. My studies showed, however, that the electron density of mitochondria and other cytoplasmic organelles was lower in samples fixed with osmium ferrocyanide than in samples fixed with osmium ferricyanide.

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