scholarly journals Isolation of Campylobacter Species from Stool Samples by Use of a Filtration Method: Assessment from a United States-Based Population

2017 ◽  
Vol 55 (7) ◽  
pp. 2204-2207 ◽  
Author(s):  
Irving Nachamkin ◽  
Phi Nguyen
Keyword(s):  
United States ◽  
Clinical Microbiology ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Filtration Method ◽  
Content Type ◽  
Stool Samples ◽  
Campylobacter Species ◽  
Clinically Significant ◽  
Microaerobic Conditions

ABSTRACT Fecal samples submitted to our clinical microbiology laboratory from patients in the Philadelphia region were prospectively analyzed for Campylobacter species other than C. jejuni and C. coli using a filtration method and microaerobic conditions with increased H 2 concentrations. Of 225 samples tested, 13 (5.8%) yielded Campylobacter species, with frequent isolation of C. concisus . The majority of Campylobacter species were not clinically significant. Additional studies in U.S. populations are warranted.

scholarly journals Molecular Assay Validation Using Genomic Sequence Databases

2016 ◽  
Vol 54 (12) ◽  
pp. 2854-2856 ◽  
Author(s):  
John P. Dekker
Keyword(s):  
Clinical Microbiology ◽  
Genomic Sequence ◽  
Sequence Data ◽  
Whole Genome Sequence ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Molecular Assay ◽  
Pcr Assay ◽  
Content Type ◽  
Sequence Databases

Whole-genome sequence databases offer newin silicoapproaches for designing and validating PCR assays in the clinical microbiology laboratory. An article in this issue of theJournal of Clinical Microbiology(M. J. Jansen van Rensburg, C. Swift, A. J. Cody, C. Jenkins, and M. C. J. Maiden, J Clin Microbiol, 54:2882–2890, 2016,http://dx.doi.org/10.1128/JCM.01522-16) demonstrates the use of publicly available genomic sequence data to evaluate a PCR assay for distinguishingCampylobacterspecies.

scholarly journals Evaluation of the Oxyrase OxyPlate Anaerobe Incubation System

2000 ◽  
Vol 38 (2) ◽  
pp. 499-507 ◽  
Author(s):  
Lois S. Wiggs ◽  
Joseph J. Cavallaro ◽  
J. Michael Miller
Keyword(s):  
Clinical Microbiology ◽  
Anaerobic Bacteria ◽  
Clinical Microbiology Laboratory ◽  
Reference Laboratory ◽  
Coding System ◽  
Microbiology Laboratory ◽  
Bacterial Strains ◽  
Incubation System ◽  
Control And Prevention ◽  
Clinically Significant

The Oxyrase OxyPlate anaerobe incubation system was evaluated for its ability to support the growth of clinically significant anaerobic bacteria previously identified by the Anaerobe Reference Laboratory at the Centers for Disease Control and Prevention. The results were compared with those obtained with conventional anaerobe blood agar plates incubated in an anaerobe chamber. We tested 251 anaerobic bacterial strains. Plates were read at 24, 48, and 72 h; growth was scored by a numerical coding system that combines the degree of growth and the colony size. Organisms (number of strains tested) used in this study were Actinomyces (32),Anaerobiospirillum (8), Bacteroides(39), Campylobacter (8), Clostridium (96),Fusobacterium (12), Leptotrichia (8),Mobiluncus (8), Peptostreptococcus(16), and Propionibacterium (24). At 24 h, 101 (40.2%) of the 251 strains tested showed better growth with the anaerobe chamber than with the OxyPlate system, 10 (4.1%) showed better growth with the OxyPlate system, and the remaining 140 (55.8%) showed equal growth with both systems. At 48 h, 173 (68.9%) showed equal growth with both systems, while 78 (31.1%) showed better growth with the anaerobe chamber. At 72 h, 176 (70.1%) showed equal growth with both systems, while 75 (29.9%) showed better growth with the anaerobe chamber. The OxyPlate system performed well for the most commonly isolated anaerobes but was inadequate for some strains. These results indicate that the Oxyrase OxyPlate system was effective in creating an anaerobic atmosphere and supporting the growth of anaerobic bacteria within 72 h. OxyPlates would be a useful addition to the clinical microbiology laboratory lacking resources for traditional anaerobic culturing techniques.

Systematic Assessment of Culture Review as a Tool to Assess Errors in the Clinical Microbiology Laboratory

2008 ◽  
Vol 132 (11) ◽  
pp. 1792-1795
Author(s):  
Nancy Goodyear ◽  
Bruce K. Ulness ◽  
Jennifer L. Prentice ◽  
Brad T. Cookson ◽  
Ajit P. Limaye
Keyword(s):  
Clinical Microbiology ◽  
Susceptibility Testing ◽  
Positive Culture ◽  
The United States ◽  
Error Rates ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Negative Culture ◽  
Clinically Significant ◽  
Potentially Clinically Significant

Abstract Context.—Daily supervisory review is a common practice in microbiology laboratories; however, there are no publications describing errors corrected by this practice. Objective.—To determine (1) the correction rates for routinely reviewed positive cultures, (2) the correction rates for negative cultures, and (3) the types of corrections that are found, including the number with potential clinical significance. Design.—We prospectively assessed errors identified during culture report review for all positive (10-month period) and negative (1-month period) cultures at a single, university-based clinical microbiology laboratory in the United States. Errors were classified using predefined categories, and total and per category error rates were determined. A χ2 test was used to assess significant differences between error rates. Results.—A total of 112 108 culture reports were examined; 914 reports required a total of 1043 corrections. Of 101 703 positive culture reports, 786 (0.8%) required 900 corrections, 302 (0.3%) of which were potentially clinically significant. Of 10 405 negative culture reports, 128 (1.2%) required 143 corrections, 5 (0.05%) of which were potentially clinically significant. The rate of potentially clinically significant errors was significantly higher among positive versus negative culture reports (P < .001). Errors from positive culture reports most commonly involved susceptibility (374 [42%]), reporting (275 [31%]), and identification workup (217 [24%]). Most potentially significant errors from positive culture reports involved susceptibility testing (n = 253) and specimens from wound or lower respiratory tract (P < .001). Conclusions.—Review of culture reports from positive cultures from nonsterile sites with special attention to antimicrobial susceptibility testing and reporting would be most likely to detect potentially significant errors within the clinical microbiology laboratory.

scholarly journals EDTA-Modified Carbapenem Inactivation Method: a Phenotypic Method for Detecting Metallo-β-Lactamase-Producing Enterobacteriaceae

2019 ◽  
Vol 57 (5) ◽  
Author(s):  
M. M. Sfeir ◽  
J. A. Hayden ◽  
K. A. Fauntleroy ◽  
C. Mazur ◽  
J. K. Johnson ◽  
...  
Keyword(s):  
Clinical Microbiology ◽  
Health Concern ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Resource Limited Settings ◽  
Content Type ◽  
Resource Limited ◽  
Phenotypic Method ◽  
Control And Prevention ◽  
Infection Control And Prevention

ABSTRACT The increase in the prevalence and impact of infections caused by carbapenemase-producing Enterobacteriaceae is a global health concern. Therefore, rapid and accurate methods to detect these organisms in any clinical microbiology laboratory, including those in resource-limited settings, are essential to prevent and contain their spread. It is also important to differentiate between serine- and metal-dependent carbapenemases elaborated by carbapenemase-producing isolates for epidemiologic, infection control and prevention, and therapeutic purposes. Here, we describe the development and evaluation of the EDTA-modified carbapenem inactivation method (eCIM), an assay for discriminating between serine- and metal-dependent (i.e., metallo-β-lactamases [MBLs]) carbapenemases when used in conjunction with the modified carbapenem inactivation method (mCIM). The eCIM had an overall sensitivity and specificity of 100% and was adopted by the Clinical and Laboratory Standards Institute as a method to use in combination with the mCIM to identify MBL-producing Enterobacteriaceae.

scholarly journals Decrease in Enteroviral Meningitis - An Unexpected Benefit of COVID-19 Mitigation?

2020 ◽  
Author(s):  
Kami D Kies ◽  
Amber S Thomas ◽  
Matthew J Binnicker ◽  
Kelli L Bashynski ◽  
Robin Patel
Keyword(s):  
Cerebrospinal Fluid ◽  
Laboratory Testing ◽  
Clinical Microbiology ◽  
Late Summer ◽  
Mitigation Strategies ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Testing Data ◽  
Early Fall ◽  
Enteroviral Meningitis

Abstract Enteroviral meningitis is seasonal, typically exhibiting a rise in prevalence in late summer/early fall. Based on clinical microbiology laboratory testing data of cerebrospinal fluid, the expected August/September/October peak in enteroviral meningitis did not occur in 2020, possibly related to COVID-19 mitigation strategies.

scholarly journals Implementation and evaluation of a novel real-time multiplex assay for SARS-CoV-2: in-field learnings from a clinical microbiology laboratory

Pathology ◽  
2020 ◽  
Vol 52 (7) ◽  
pp. 754-759 ◽  
Author(s):  
Eloise Williams ◽  
Katherine Bond ◽  
Brian Chong ◽  
Dawn Giltrap ◽  
Malcolm Eaton ◽  
...  
Keyword(s):  
Real Time ◽  
Clinical Microbiology ◽  
Multiplex Assay ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory

scholarly journals One Small Step for the Gram Stain, One Giant Leap for Clinical Microbiology

2016 ◽  
Vol 54 (6) ◽  
pp. 1416-1417 ◽  
Author(s):  
Richard B. Thomson
Keyword(s):  
Clinical Microbiology ◽  
Rapid Test ◽  
Error Rates ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Gram Stain ◽  
Small Step ◽  
Link Type ◽  
Giant Leap

The Gram stain is one of the most commonly performed tests in the clinical microbiology laboratory, yet it is poorly controlled and lacks standardization. It was once the best rapid test in microbiology, but it is no longer trusted by many clinicians. The publication by Samuel et al. (J. Clin. Microbiol. 54:1442–1447, 2016,http://dx.doi.org/10.1128/JCM.03066-15) is a start for those who want to evaluate and improve Gram stain performance. In an age of emerging rapid molecular results, is the Gram stain still relevant? How should clinical microbiologists respond to the call to reduce Gram stain error rates?

scholarly journals Demonstration of Cord Formation by Rough Mycobacterium abscessus Variants: Implications for the Clinical Microbiology Laboratory: Fig. 1.

2011 ◽  
Vol 49 (6) ◽  
pp. 2293-2295 ◽  
Author(s):  
Alejandro Sánchez-Chardi ◽  
Francesc Olivares ◽  
Thomas F. Byrd ◽  
Esther Julián ◽  
Cecilia Brambilla ◽  
...  
Keyword(s):  
Clinical Microbiology ◽  
Mycobacterium Abscessus ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory
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