scholarly journals Implementation and evaluation of a novel real-time multiplex assay for SARS-CoV-2: in-field learnings from a clinical microbiology laboratory

Pathology ◽  
2020 ◽  
Vol 52 (7) ◽  
pp. 754-759 ◽  
Author(s):  
Eloise Williams ◽  
Katherine Bond ◽  
Brian Chong ◽  
Dawn Giltrap ◽  
Malcolm Eaton ◽  
...  
Keyword(s):  
Real Time ◽  
Clinical Microbiology ◽  
Multiplex Assay ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory

Development, implementation, and trend analysis of real-time PCR tests for the clinical microbiology laboratory

2004 ◽  
Vol 26 (19) ◽  
pp. 145-153 ◽  
Author(s):  
Thomas F. Smith ◽  
James R. Uhl ◽  
Mark J. Espy ◽  
Lynne M. Sloan ◽  
Emily A. Vetter ◽  
...  
Keyword(s):  
Trend Analysis ◽  
Real Time ◽  
Real Time Pcr ◽  
Clinical Microbiology ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory

scholarly journals Real-time laboratory surveillance of sexually-transmissible infections in Marseille University hospitals reveals rise of gonorrhoea, syphilis and human immunodeficiency virus seroconversions in 2012

2013 ◽  
Vol 18 (7) ◽  
Author(s):  
P Colson ◽  
F Gouriet ◽  
S Badiaga ◽  
C Tamalet ◽  
A Stein ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Real Time ◽  
Clinical Microbiology ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
University Hospitals ◽  
Sexually Transmitted ◽  
Sexually Transmissible Infections ◽  
Immunodeficiency Virus ◽  
Laboratory Surveillance

Real-time systematic monitoring of the number of infections diagnosed in our clinical microbiology laboratory in Marseille recently drew attention to the fact that the incidence of gonorrhoea was 10-fold greater from September through December 2012 than during same months of previous years. We also found an increase in the annual incidence of syphilis and human immunodeficiency virus seroconversion. Our system allowed timely identification of an increase in sexually-transmitted infections in Marseille for the whole year of 2012.

Application of Rapid-Cycle Real-Time Polymerase Chain Reaction for Diagnostic Testing in the Clinical Microbiology Laboratory

2003 ◽  
Vol 127 (9) ◽  
pp. 1112-1120 ◽  
Author(s):  
Franklin R. Cockerill
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Real Time Pcr ◽  
Clinical Microbiology ◽  
Diagnostic Testing ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Conventional Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract Context.—Rapid-cycle real-time polymerase chain reaction (PCR) technology combines rapid thermocycling with real-time fluorescent probe detection of amplified target nucleic acids. Objectives.—To review and compare the method of rapid-cycle real-time PCR to conventional PCR methods. To describe the application of rapid-cycle real-time PCR for diagnostic testing in the microbiology laboratory. Data Selection.—Information is presented from published literature as well as from personal experience at the Mayo Clinical Microbiology Laboratory (Rochester, Minn). Conclusions.—Compared to conventional PCR methods, rapid-cycle real-time PCR diagnostics are much faster and easier to perform, and, because both PCR and probe detection occur in the same reaction vessel, the possibility of amplified product (amplicon) contamination is lessened. Furthermore, compared to conventional culture-based or direct antigen detection methods, rapid-cycle real-time PCR assays are frequently more sensitive and much more rapid techniques for detecting or quantifying microorganisms in human samples and for identifying genes or mutations in pathogens associated with antimicrobial resistance.

scholarly journals Decrease in Enteroviral Meningitis - An Unexpected Benefit of COVID-19 Mitigation?

2020 ◽  
Author(s):  
Kami D Kies ◽  
Amber S Thomas ◽  
Matthew J Binnicker ◽  
Kelli L Bashynski ◽  
Robin Patel
Keyword(s):  
Cerebrospinal Fluid ◽  
Laboratory Testing ◽  
Clinical Microbiology ◽  
Late Summer ◽  
Mitigation Strategies ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Testing Data ◽  
Early Fall ◽  
Enteroviral Meningitis

Abstract Enteroviral meningitis is seasonal, typically exhibiting a rise in prevalence in late summer/early fall. Based on clinical microbiology laboratory testing data of cerebrospinal fluid, the expected August/September/October peak in enteroviral meningitis did not occur in 2020, possibly related to COVID-19 mitigation strategies.

scholarly journals One Small Step for the Gram Stain, One Giant Leap for Clinical Microbiology

2016 ◽  
Vol 54 (6) ◽  
pp. 1416-1417 ◽  
Author(s):  
Richard B. Thomson
Keyword(s):  
Clinical Microbiology ◽  
Rapid Test ◽  
Error Rates ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Gram Stain ◽  
Small Step ◽  
Link Type ◽  
Giant Leap

The Gram stain is one of the most commonly performed tests in the clinical microbiology laboratory, yet it is poorly controlled and lacks standardization. It was once the best rapid test in microbiology, but it is no longer trusted by many clinicians. The publication by Samuel et al. (J. Clin. Microbiol. 54:1442–1447, 2016,http://dx.doi.org/10.1128/JCM.03066-15) is a start for those who want to evaluate and improve Gram stain performance. In an age of emerging rapid molecular results, is the Gram stain still relevant? How should clinical microbiologists respond to the call to reduce Gram stain error rates?

scholarly journals Demonstration of Cord Formation by Rough Mycobacterium abscessus Variants: Implications for the Clinical Microbiology Laboratory: Fig. 1.

2011 ◽  
Vol 49 (6) ◽  
pp. 2293-2295 ◽  
Author(s):  
Alejandro Sánchez-Chardi ◽  
Francesc Olivares ◽  
Thomas F. Byrd ◽  
Esther Julián ◽  
Cecilia Brambilla ◽  
...  
Keyword(s):  
Clinical Microbiology ◽  
Mycobacterium Abscessus ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory
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