scholarly journals First Isolation of SCCmec IV- and Panton-Valentine Leukocidin-Positive, Sequence Type 8, Community-Associated Methicillin-Resistant Staphylococcus aureus in Israel

2010 ◽  
Vol 48 (10) ◽  
pp. 3827-3828 ◽  
Author(s):  
D. Glikman ◽  
S. Davidson ◽  
R. Kudinsky ◽  
Y. Geffen ◽  
H. Sprecher
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sequence Type ◽  
Positive Sequence ◽  
Methicillin Resistant ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

scholarly journals Panton-Valentine leukocidin positive MRSA in 2003: the Dutch situation

2004 ◽  
Vol 9 (11) ◽  
pp. 3-4 ◽  
Author(s):  
W J B Wannet ◽  
M E Heck ◽  
G N Pluister ◽  
E Spalburg ◽  
M G Van Santen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
The Netherlands ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sequence Type ◽  
Hospital Environment ◽  
Molecular Subtyping ◽  
Methicillin Resistant ◽  
Global Epidemic ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolates in the Netherlands in 2003 revealed that 8% of the hospital isolates carried the loci for Panton-Valentine leukocidin (PVL). Molecular subtyping showed that most Dutch PVL-MRSA genotypes corresponded to well-documented global epidemic types. The most common PVL-MRSA genotypes were sequence type ST8, ST22, ST30, ST59 and ST80. MRSA with ST8 increased in the Netherlands from 1% in 2002 to 17% in 2003. It is emphasised that PVL-MRSA might not only emerge in the community, but also in the hospital environment.

scholarly journals Draft Genome Sequences of Eight Community-Associated Methicillin-Resistant Staphylococcus aureus Strains

2021 ◽  
Vol 10 (46) ◽  
Author(s):  
Kidon Sung ◽  
Dereje D. Gudeta ◽  
Miseon Park ◽  
Paula Snippes Vagnone ◽  
Mohamed S. Nawaz ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Draft Genome ◽  
The United States ◽  
Sequence Type ◽  
Genome Sequences ◽  
Methicillin Resistant ◽  
Content Type ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Here, we report the draft genome sequences of eight community-associated methicillin-resistant Staphylococcus aureus strains that were resistant to cefoxitin, ampicillin, and erythromycin. Three isolates, i.e., CAR1, CAR2, and CAR8, were sequence type 8 (ST8) with staphylococcal cassette chromosome mec (SCC mec ) type IVa and were Panton-Valentine leukocidin (PVL) positive, which has been known as a predominant clone in the United States.

scholarly journals Identification and Characterization of the Multidrug Resistance Gene cfr in a Panton-Valentine Leukocidin-Positive Sequence Type 8 Methicillin-Resistant Staphylococcus aureus IVa (USA300) Isolate

2010 ◽  
Vol 54 (12) ◽  
pp. 4978-4984 ◽  
Author(s):  
Anna C. Shore ◽  
Orla M. Brennan ◽  
Ralf Ehricht ◽  
Stefan Monecke ◽  
Stefan Schwarz ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Resistance Gene ◽  
Sequence Type ◽  
Conjugative Plasmid ◽  
Positive Sequence ◽  
Transposase Gene ◽  
Methicillin Resistant ◽  
Antimicrobial Resistance Genes ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

ABSTRACT The staphylococcal cfr gene mediates resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A, a phenotype that has been termed PhLOPSA. The cfr gene has mainly been associated with coagulase-negative staphylococcal isolates from animals, and only a few cfr-positive methicillin-resistant Staphylococcus aureus (MRSA) isolates have been described so far. This study reports the first description of a cfr-positive MRSA isolate (M05/0060) belonging to the pandemic Panton-Valentine leukocidin (PVL)-positive sequence type 8 MRSA IVa/USA300 (ST8-MRSA-IVa/USA300) clone. The cfr gene was detected in M05/0060 using a DNA microarray which was used to screen PVL-positive MRSA isolates for the presence of virulence genes, typing markers, and antimicrobial resistance genes. Antimicrobial susceptibility testing revealed that M05/0060 exhibited the cfr-associated resistance phenotype. Molecular analysis identified the presence of cfr and a second phenicol resistance gene, fexA, on a novel 45-kb conjugative plasmid, which was designated pSCFS7. Within pSCFS7, a DNA segment consisting of cfr, a truncated copy of insertion sequence IS21-558, and a region with homology to the DNA invertase gene bin3 of transposon Tn552 from Bacillus mycoides was integrated into the transposase gene tnpB of the fexA-carrying transposon Tn558. The emergence of a multidrug-resistant cfr-positive variant of ST8-MRSA-IVa/USA300 is alarming and requires ongoing surveillance. Moreover, the identification of a novel conjugative plasmid carrying the cfr gene indicates the ability of cfr to spread to other MRSA strains.

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