scholarly journals Performance of the Verigene Gram-Positive Blood Culture Assay for Direct Detection of Gram-Positive Organisms and Resistance Markers in a Pediatric Hospital

2013 ◽  
Vol 52 (1) ◽  
pp. 283-287 ◽  
Author(s):  
J. Mestas ◽  
C. M. Polanco ◽  
S. Felsenstein ◽  
J. Dien Bard
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Direct Detection ◽  
Pediatric Hospital ◽  
Gram Positive ◽  
Resistance Markers

scholarly journals Clinical Evaluation of the iCubate iC-GPC Assay for Detection of Gram-Positive Bacteria and Resistance Markers from Positive Blood Cultures

2018 ◽  
Vol 56 (9) ◽  
Author(s):  
Paul A. Granato ◽  
Melissa M. Unz ◽  
Raymond H. Widen ◽  
Suzane Silbert ◽  
Stephen Young ◽  
...  
Keyword(s):  
Blood Culture ◽  
Staphylococcus Epidermidis ◽  
Bloodstream Infections ◽  
Blood Cultures ◽  
Gram Positive ◽  
Content Type ◽  
Gram Positive Bacteria ◽  
Sequencing Method ◽  
Resistance Markers ◽  
Gram Positive Cocci

ABSTRACT The iC-GPC Assay (iCubate, Huntsville, AL) is a qualitative multiplex test for the detection of five of the most common Gram-positive bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Enterococcus faecalis, and Enterococcus faecium) responsible for bacterial bloodstream infections, performed directly from positive blood cultures. The assay also detects the presence of the mecA, vanA, and vanB resistance determinants. This study comparatively evaluated the performance of the iC-GPC Assay against the Verigene Gram-positive blood culture (BC-GP) assay (Luminex Corp., Austin, TX) for 1,134 patient blood culture specimens positive for Gram-positive cocci. The iC-GPC Assay had an overall percent agreement with the BC-GP assay of 95.5%. Discordant specimens were further analyzed by PCR and a bidirectional sequencing method. The results indicate that the iC-GPC Assay together with the iCubate system is an accurate and reliable tool for the detection of the five most common Gram-positive bacteria and their resistance markers responsible for bloodstream infections.

VERIGENE GRAM-POSITIVE BLOOD CULTURE TESTING: CLINICAL AND PHARMACOECONOMIC IMPACT

CHEST Journal ◽  
2018 ◽  
Vol 154 (4) ◽  
pp. 835A
Author(s):  
GEORGE UDEANI ◽  
HOA NGO ◽  
UCHE MBADUGHA ◽  
FRANCES CEPEDA ◽  
SALIM SURANI
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Gram Positive

scholarly journals Direct detection ofmecA,blaSHV, blaCTX-M,blaTEMandblaOXAgenes from positive blood culture bottles by multiplex-touchdown PCR assay

2017 ◽  
Vol 64 (2) ◽  
pp. 138-143 ◽  
Author(s):  
M.-Y. Wang ◽  
J.-L. Geng ◽  
Y.-J. Chen ◽  
Y. Song ◽  
M. Sun ◽  
...  
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Direct Detection ◽  
Pcr Assay ◽  
Touchdown Pcr

scholarly journals Use of thermonuclease testing to identify Staphylococcus aureus by direct examination of blood cultures

2003 ◽  
Vol 9 (1-2) ◽  
pp. 185-190
Author(s):  
N. M. Kaplan
Keyword(s):  
Staphylococcus Aureus ◽  
Blood Culture ◽  
Direct Detection ◽  
Blood Cultures ◽  
Military Hospital ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Direct Examination ◽  
Gram Positive ◽  
Gram Positive Cocci

Blood cultures submitted to the Clinical Microbiology Laboratory, Queen Alia Military Hospital, Amman during 1999-2001 were examined to evaluate thermonuclease testing for identifying Staphylococcus aureus in blood culture broths growing gram-positive cocci. Of 170 cultures studied, 129 yielded gram-positive staphylococci and 41 yielded other gram-positive cocci. Toluidine blue-deoxynucleic acid agar plates were used to test for thermonuclease activity. St and ard tube coagulase tests were performed on the isolates. Direct detection of thermonuclease activity in 76 blood culture broths containing gram-positive staphylococci showed 100% correlation with subsequent tube coagulase tests. The thermonuclease test provides a fast, specific and reliable confirmation of S. aureus bacteraemia by direct examination of blood culture broths that contain gram-positive cocci. This allows for timely, optimal antibiotic therapy

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