scholarly journals Clinical and Molecular Characterization of Invasive Heteroresistant Vancomycin-Intermediate Staphylococcus aureus Infections in Korean Hospitals

2015 ◽  
Vol 54 (3) ◽  
pp. 760-763 ◽  
Author(s):  
Eu Suk Kim ◽  
In-Gyu Bae ◽  
Jeong Eun Cho ◽  
Yun Jung Choi ◽  
Il-Hwan Kim ◽  
...  
Keyword(s):  
Health Care ◽  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Sequence Type ◽  
Content Type ◽  
Health Care Associated Infections ◽  
Persistent Bacteremia

Invasive heteroresistant vancomycin-intermediateStaphylococcus aureus(h-VISA) isolates were identified and characterized in 10 Korean hospitals from July 2009 to June 2011. The prevalence of h-VISA infections was 3.3% (42/1,289). Most (41/42) were health care-associated infections caused by strains belonging to sequence type 5. Cases of persistent bacteremia were frequent (17/42), and 30-day mortality was high (16/40).

scholarly journals Globally Expanding Carbapenemase Finally Appears in Spain: Nosocomial Outbreak of Acinetobacter baumannii Producing Plasmid-Encoded OXA-23 in Barcelona, Spain

2013 ◽  
Vol 57 (10) ◽  
pp. 5155-5157 ◽  
Author(s):  
Noraida Mosqueda ◽  
Paula Espinal ◽  
Clara Cosgaya ◽  
Sergio Viota ◽  
Virginia Plasensia ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Molecular Characterization ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Clinical Isolates ◽  
Nosocomial Outbreak ◽  
Content Type ◽  
Hospital Outbreak

ABSTRACTResistance ofAcinetobacter baumanniiclinical isolates to carbapenems is on the rise worldwide mainly in association with the production of OXA-23. Until recently, however, OXA-23 was absent in Spain. In this work, we report the molecular characterization of a hospital outbreak of OXA-23-producingA. baumanniiin Barcelona caused by a multidrug-resistant (MDR) clone belonging to international clone IC-II/sequence type ST85 between October 2010 and May 2011.blaOXA-23was carried in a plasmid of 90 kb and located within the composite transposon Tn2006.

scholarly journals Molecular Characterization of a Catalase-Negative Staphylococcus aureus Blood Culture Isolate: TABLE 1

2015 ◽  
Vol 53 (11) ◽  
pp. 3699-3701 ◽  
Author(s):  
Jeanette W. P. Teo ◽  
Steven Kum ◽  
Roland Jureen ◽  
Raymond T. P. Lin
Keyword(s):  
Amino Acids ◽  
Staphylococcus Aureus ◽  
Blood Culture ◽  
Molecular Characterization ◽  
Frameshift Mutation ◽  
Gene Encoding ◽  
Blood Culture Isolate ◽  
Content Type ◽  
Culture Isolate

Here we report a catalase-negative methicillin-sensitiveStaphylococcus aureusisolate collected from a blood culture. Sequencing through the gene encoding catalase,katA, demonstrated a 2-bp insertion. The resulting frameshift mutation generates a protein that has lost 26 amino acids (aa) at its C-terminal domain.

scholarly journals Molecular Characterization of Carbapenemase-Producing Pseudomonas aeruginosa of Czech Origin and Evidence for Clonal Spread of Extensively Resistant Sequence Type 357 Expressing IMP-7 Metallo-β-Lactamase

2017 ◽  
Vol 61 (12) ◽  
Author(s):  
Costas C. Papagiannitsis ◽  
Matej Medvecky ◽  
Katerina Chudejova ◽  
Anna Skalova ◽  
Veronika Rotova ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Molecular Characterization ◽  
Genomic Island ◽  
Sequence Type ◽  
The Other ◽  
Sequencing Data ◽  
Molecular Surveillance ◽  
Content Type ◽  
Clonal Spread

ABSTRACT The objective of this study was to perform molecular surveillance for assessing the spread of carbapenemase-producing Pseudomonas aeruginosa in Czech hospitals. One hundred thirty-six carbapenemase-producing isolates were recovered from 22 hospitals located throughout the country. Sequence type 357 (ST357) dominated (n = 120) among carbapenemase producers. One hundred seventeen isolates produced IMP-type (IMP-7 [n = 116] and IMP-1 [n = 1]) metallo-β-lactamases (MβLs), 15 produced the VIM-2 MβL, and the remaining isolates expressed the GES-5 enzyme. The bla IMP-like genes were located in three main integron types, with In-p110-like being the most prevalent (n = 115). The two other IMP-encoding integrons (In1392 and In1393) have not been described previously. bla VIM-2-carrying integrons included In59-like, In56, and a novel element (In1391). bla GES-5 was carried by In717. Sequencing data showed that In-p110-like was associated with a Tn4380-like transposon inserted in genomic island LESGI-3 in the P. aeruginosa chromosome. The other integrons were also integrated into the P. aeruginosa chromosome. These findings indicated the clonal spread of ST357 P. aeruginosa, carrying the IMP-7-encoding integron In-p110, in Czech hospitals. Additionally, the sporadic emergence of P. aeruginosa producing different carbapenemase types, associated with divergent or novel integrons, punctuated the ongoing evolution of these bacteria.

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