Ammonium bicarbonate as a replacement for carbon dioxide in Transgrow bottles for primary isolation of Neisseria gonorrhoeae.

1983 ◽  
Vol 18 (5) ◽  
pp. 1258-1259 ◽  
Author(s):  
L D Potter ◽  
J S Lewis ◽  
B B Wentworth ◽  
E H Larsen
Keyword(s):  
Carbon Dioxide ◽  
Neisseria Gonorrhoeae ◽  
Ammonium Bicarbonate ◽  
Primary Isolation

Carbon dioxide requirement of Neisseria gonorrhoeae growing on a solid medium

1976 ◽  
Vol 4 (2) ◽  
pp. 129-132
Author(s):  
D J Platt
Keyword(s):  
Carbon Dioxide ◽  
Neisseria Gonorrhoeae ◽  
Rapid Growth ◽  
Solid Medium ◽  
Colony Growth ◽  
Growth Cycle ◽  
Lag Phase ◽  
Replica Plating ◽  
Early Stages ◽  
Male Patients

A survey of 120 isolations of Neisseria gonorrhoeae from male patients showed that 47 (39%) isolates were unable to produce visible colonies without a supply of exogenous carbon dioxide. Of 63 strains, 25 strains required CO2 for isolation but none exhibited a CO2 requirement beyond subculture 4. CO2-requiring strains deprived of CO2 appeared to lose their colony-forming ability exponentially in an environment otherwise conductive to growth. The CO2 requirement was found to be linked to the initiation of growth. An agar-to-agar replica-plating device was used to study the early stages of colonial growth. The CO2 requirement was also found to correlate with the various phases of the colony growth cycle, such that it was required during lag phase, not required during the phase of rapid growth, and returned as colonies aged. These results are discussed.

scholarly journals The Effects of Different Formulation of Baking Powder on Short Biscuits Characteristics

2011 ◽  
Vol 68 (2) ◽  
Author(s):  
Georgiana CIOROIANU ◽  
Claudia Felicia OGNEAN ◽  
Mihai OGNEAN
Keyword(s):  
Carbon Dioxide ◽  
Elevated Temperature ◽  
Tartaric Acid ◽  
Ammonium Bicarbonate ◽  
Potassium Bicarbonate ◽  
Baked Goods ◽  
Sodium Acid Pyrophosphate

Chemical leaveners are used to give cookies, cakes, and other baked goods their characteristic textures. They produce gas when a carbon dioxide source and an acid are mixed together and come into contact with water. The most common sources of gas are sodium or potassium bicarbonate alone or in combination with ammonium bicarbonate. A great variety of acids are used in baking powder formulations. The acid are classified according to their capacity to react at lower or elevated temperature as rapid or slow acting. The aim of these study is to evaluate a very rapid acting acid (tartaric acid) and a slow to very slow acting acid (sodium acid pyrophosphate - SAPP) on the characteristics of short biscuits. The acids were added to reach 0, 25%, 50%, 75% and 100% of neutralizing values (VN). The biscuits height and diameter, alkalinity and sensorial profile were determined.

Solidification of Greenhouse Gas Carbon Dioxide

2014 ◽  
Vol 472 ◽  
pp. 879-883
Author(s):  
Wei Yin
Keyword(s):  
Carbon Dioxide ◽  
Calcium Carbonate ◽  
Ambient Temperature ◽  
Calcium Sulfate ◽  
Exothermic Reaction ◽  
Ammonium Bicarbonate ◽  
Second Order Reaction ◽  
Carbon Dioxide Gas ◽  
Water Media ◽  
Closed Reactor

The feasible routine of carbon dioxide solidification is developed, which employs carbon dioxide with calcium sulfate and ammonia to obtain calcium carbonate and ammonium sulfate at ambient temperature. The process of carbon dioxide solidification is a spontaneous and exothermic reaction, whose possesses the rate constants of the second order reaction, which can be attributed to carbon dioxide gas dissolving the water media and carbon dioxide reacted with ammonia to produce ammonium bicarbonate. Calcium sulfate changing rapidly into calcium carbonate accelerates the process of reaction of carbon dioxide reacted with ammonia. The optimization process parameters of carbon dioxide solidification are a 0.075-MPa of CO2, a 0.5-mol of CaSO4·2H2O, a 0.5-mol of NH3·H2O and a 100-ml of H2O in a closed reactor, which is able to obtain 100% CO2 gas solidification efficiency within 4 minutes at ambient temperature.

scholarly journals Genetic Transformation as a tool for detection of Neisseria gonorrhoeae

1976 ◽  
Vol 4 (1) ◽  
pp. 71-81
Author(s):  
A Janik ◽  
E Juni ◽  
G A Heym
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Genetic Transformation ◽  
Rapid Method ◽  
Deoxyribonucleic Acid ◽  
Time Interval ◽  
Diagnostic Laboratory ◽  
Primary Isolation ◽  
Transformation Assay ◽  
Mutant Strains ◽  
Pure Cultures

A rapid method for the detection of Neisseria gonorrhoeae, making use of the ability of deoxyribonucleic acid samples from clinically isolated strains of this organism to transform nutritional mutants of a particular strain of N. gonorrhoeae, has been described. In addition to using isolated cultures, transforming deoxyribonucleic acid can be obtained directly from the material that adheres to swabs of the cervix or the urethra. The time interval for transfer of swabs to the diagnostic laboratory is not a significant factor. It is not necessary to use pure cultures on primary isolation plates to obtain definitive results. Nongonorrhoeae neisserias, as well as a large variety of commonly encountered unrelated bacteria, do not react or interfere in the transformation assay when using one of the mutant strains under a standardized set of conditions. The entire assay can be completed in less than 24 h. It has also been shown that type T4 cells of the strain of N. gonorrhoeae employed in the present study are competent for genetic transformation, although type T4 cells are transformed at a significantly lower frequency than are type T2 cells of the same strain.

Porous Ti-6Al-4V Fabricated by Powder Sintering and its Property Evaluation

2011 ◽  
Vol 396-398 ◽  
pp. 257-260
Author(s):  
Rui Hua Hu ◽  
Cun Fu Yan ◽  
Jae Kyoo Lim
Keyword(s):  
Carbon Dioxide ◽  
Weight Ratio ◽  
Porous Titanium ◽  
Ammonium Bicarbonate ◽  
Decomposition Products ◽  
Space Holder ◽  
Porous Ti ◽  
Powder Sintering ◽  
Property Evaluation ◽  
Sintering Method

Porous titanium alloys are frequently used as bio-implant scaffold. In this research, porous Ti-6Al-4V was fabricated by using powder sintering method with ammonium bicarbonate powders as space holder. The space holder decomposes to gases like ammonia, carbon dioxide and water at comparatively low temperature. The advantages of this pore holder and its decomposition products are that they have no contamination to host powders, and harmless to sintering machine. Green bodies with different weight ratio of pore holder and host powder are prepared, and then the pore holders are fully removed by heating at 200°C. Porosity, density, compressive strength and modulus of the sintered porous Ti-6Al-4V are tested.

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