scholarly journals Seroepidemiology of Human Group C Rotavirus in South Africa

1999 ◽  
Vol 37 (12) ◽  
pp. 4142-4144 ◽  
Author(s):  
A. D. Steele ◽  
V. L. A. James
Keyword(s):  
South Africa ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Population ◽  
Human Group ◽  
South Africans ◽  
Group C Rotavirus ◽  
Percent Positivity ◽  
Rotavirus Infections ◽  
Rotavirus Vp6

Sera from three separate healthy population cohorts were used to determine the incidence of group C rotavirus infections in 1,356 South Africans. Using an enzyme-linked immunosorbent assay based on a recombinant group C rotavirus VP6 protein, the total percent positivity was found to be 34.4% (range, 33 to 38%), with almost half of the population infected after the age of 20 years.

scholarly journals Enzyme-Linked Immunosorbent Assay Based on Recombinant Human Group C Rotavirus Inner Capsid Protein (VP6) To Detect Human Group C Rotaviruses in Fecal Samples

1998 ◽  
Vol 36 (11) ◽  
pp. 3178-3181 ◽  
Author(s):  
V. L. A. James ◽  
P. R. Lambden ◽  
E. O. Caul ◽  
, and I. N. Clarke
Keyword(s):  
Immunosorbent Assay ◽  
Polyacrylamide Gel Electrophoresis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Group ◽  
Fecal Samples ◽  
Reverse Transcription Pcr ◽  
The United Kingdom ◽  
Group C Rotavirus ◽  
Group A ◽  
Rotavirus Infections

A recent study showed that 43% of a population in the United Kingdom were seropositive for group C rotavirus. The higher than expected incidence may be due to limited diagnosis of acute human group C rotavirus infections because no routine test is available. Human group C rotavirus infections are routinely diagnosed by electron microscopy (EM) and a negative group A rotavirus enzyme-linked immunosorbent assay (ELISA) result. An antigen-detection ELISA was developed with hyperimmune antibodies raised to human group C rotavirus recombinant VP6 (Bristol strain) expressed in insect cells. The assay was used to screen fecal samples to determine the prevalence of group C rotavirus infection. Samples positive by ELISA were confirmed by EM, polyacrylamide gel electrophoresis of double-stranded RNA, or detection of the VP6 gene by reverse transcription-PCR. Retrospective analysis indicated a 1 to 2% detection rate of positivity among samples from patients with acute diarrhea.

scholarly journals Seroepidemiology of Human Group C Rotavirus in Japan Based on a Blocking Enzyme-Linked Immunosorbent Assay

2001 ◽  
Vol 8 (1) ◽  
pp. 161-165 ◽  
Author(s):  
Mitsutaka Kuzuya ◽  
Ritsushi Fujii ◽  
Masako Hamano ◽  
Ritsuko Ohata ◽  
Hajime Ogura ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Age Groups ◽  
Neutralization Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Age Group ◽  
Human Group ◽  
Serum Samples ◽  
Group C Rotavirus ◽  
Group A ◽  
Okayama Prefecture

ABSTRACT A novel blocking enzyme-linked immunosorbent assay (BL-ELISA) was developed for detection of antibodies to human group C rotavirus (CHRV). The specificity of the BL-ELISA was confirmed by using animal sera hyperimmunized to group A and group C rotaviruses and paired sera from five patients with acute CHRV gastroenteritis. Furthermore, there was concordance between the BL-ELISA and a neutralization assay for CHRV in 226 (95%) of 238 samples. By using the BL-ELISA, we determined the seroprevalence of CHRV in 704 serum samples obtained from nine different age groups of inhabitants of Okayama Prefecture, Japan, in 1992, 1994, and 1996. As a result, 211 sera (30%) were found to be positive for CHRV antibodies. The seroprevalence gradually increased with age and reached 52.7% in the oldest individuals. A further analysis of the youngest age group suggested that CHRVs predominantly prevail in persons older than 3 years of age in Japan. When comparing the three sampling years, a larger percentage of antibody-positive sera was detected in 1994 than in either 1992 or 1996 in individuals between 6 and 15 years of age, reflecting the occurrence of a CHRV outbreak among children during the winter of 1992 to 1993 that was previously documented. These results indicate that CHRV infections may occur more frequently in spite of the relatively low detection rate of the virus.

scholarly journals Concomitant rotavirus serotypes 1 and 4 infections in a diarrhoeic child from Belém, Brazil

1996 ◽  
Vol 38 (4) ◽  
pp. 249-252 ◽  
Author(s):  
J.D.P. Mascarenhas ◽  
R.H.P. Gusmão ◽  
Y.B. Gabbay ◽  
T.A.F. Monteiro ◽  
J.B. Gomes ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Female Child ◽  
Polyacrylamide Gel ◽  
Current Concept ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rotavirus Vaccine ◽  
Rotavirus Infection ◽  
Diarrhoeic Child ◽  
Rotavirus Infections

Concomitant serotypes 1 and 4 infections were detected in a 15-month old female child with community-acquired diarrhoea which lasted 7 days and coursed with moderate dehydration. The evidence for dual rotavirus infection was offered by the following findings: a) enzyme-linked immunosorbent assay (ELISA) positive reactions to both 1 and 4 serotypes; and b) extra-migrating bands at electro-phoresis of RNA in polyacrylamide gel (PAGE). These results suggest that children living under poor sanitation conditions are heavily exposed to rotavirus infections; in addition, the co-circulation of different serotypes in the same setting sustains the current concept that a rotavirus vaccine should be rnultivalent, in order to protect children against the four epidemiologically important rotavirus G serotypes.

Incidence and prevalence of human group C rotavirus infections in Argentina

2002 ◽  
Vol 67 (1) ◽  
pp. 106-112 ◽  
Author(s):  
Alejandro A. Castello ◽  
Marcelo H. Argüelles ◽  
Guillermo A. Villegas ◽  
Alicia Olthoff ◽  
Graciela Glikmann
Keyword(s):  
Human Group ◽  
Group C Rotavirus ◽  
Rotavirus Infections

scholarly journals Detection of Antibodies against Epidermodysplasia Verruciformis-Associated Canine Papillomavirus 3 in Sera of Dogs from Europe and Africa by Enzyme-Linked Immunosorbent Assay

2008 ◽  
Vol 16 (1) ◽  
pp. 66-72 ◽  
Author(s):  
C. E. Lange ◽  
K. Tobler ◽  
C. Favrot ◽  
M. Müller ◽  
J. O. Nöthling ◽  
...  
Keyword(s):  
South Africa ◽  
Fusion Protein ◽  
Immunosorbent Assay ◽  
Clinical Manifestations ◽  
Epidermodysplasia Verruciformis ◽  
Capsid Proteins ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Antigens ◽  
Specific Antibodies

ABSTRACT The role of papillomaviruses (PVs) in the development of canine cancers is controversial. However, recently a novel canine PV (CPV3) was detected in a dog affected with a condition reminiscent of epidermodysplasia verruciformis (EV). The aim of the present study was to investigate the seroprevalence of CPV3 by using generic enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies against either canine oral PV (COPV) or CPV3. Therefore, the capsid proteins of both PV types were expressed as glutathione S-transferase fusion protein antigens and adsorbed to glutathione-casein-coated ELISA plates. After showing that PV type-specific antibodies could be detected in the sera from dogs with confirmed COPV or CPV3 infection, CPV3- and COPV-seropositive samples were detected in two sets of canine sera collected in Switzerland and South Africa, respectively. We found specific antibodies against COPV and CPV3 among the tested sera and also a large number that were positive for both antigens. The seroprevalences of PV antibodies of 21.9% (COPV) and 26.9% (CPV3) among the tested dogs from South Africa were higher than those among the dogs from Switzerland at 10.5% (COPV) and 1.3% (CPV3). Our data suggest a need for further CPV-related seroepidemiological surveys in different countries, especially in the context of clinical manifestations and possible breed predispositions. For this purpose, the newly developed ELISAs can be a useful tool.

scholarly journals Evaluation of the immunogenicity of a recombinant HSV-1 vector expressing human group C rotavirus VP6 protein

2018 ◽  
Vol 256 ◽  
pp. 24-31
Author(s):  
Rosana P. Rota ◽  
Carlos A. Palacios ◽  
C. Facundo Temprana ◽  
Marcelo H. Argüelles ◽  
Marcelo G. Mandile ◽  
...  
Keyword(s):  
Human Group ◽  
Group C Rotavirus ◽  
Rotavirus Vp6 ◽  
Hsv 1

scholarly journals Relevance of Commercial Diagnostic Tests to Detection of Enteric Adenovirus Infections in South Africa

2000 ◽  
Vol 38 (4) ◽  
pp. 1661-1663 ◽  
Author(s):  
P. L. Moore ◽  
A. D. Steele ◽  
J. J. Alexander
Keyword(s):  
South Africa ◽  
Monoclonal Antibodies ◽  
Enzyme Immunoassay ◽  
Immunosorbent Assay ◽  
Diagnostic Tests ◽  
Significant Proportion ◽  
Adenovirus Type ◽  
Enzyme Linked Immunosorbent Assay ◽  
Enteric Adenovirus ◽  
Enteric Adenoviruses

The prevalence of enteric adenoviruses detected by an in-house enzyme-linked immunosorbent assay (the RIVM-ELISA) ranged from 13 to 38%, and subgroup F adenoviruses comprised 86%. All subgroup F adenoviruses reacted with both RIVM anti-adenovirus type 40 (Ad40) and anti-adenovirus type 41 (Ad41) monoclonal antibodies but were not detected by Adenoclone Type 40/41 enzyme immunoassay (EIA). The correlation between the Biotrin EIA and RIVM-ELISA results was low (26%). Immunospecific tests suggest that a significant proportion of enteric adenoviruses, possibly comprising previously unidentified or emerging types, are not detected by commercial diagnostic tests in South Africa.

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