scholarly journals VP4 and VP7 Genotyping by Reverse Transcription-PCR of Human Rotavirus in Mexican Children with Acute Diarrhea

2000 ◽  
Vol 38 (10) ◽  
pp. 3876-3878 ◽  
Author(s):  
Araceli Rodríguez Castillo ◽  
Andrés Velasco Villa ◽  
JoséErnesto Ramírez González ◽  
Elvira Mayén Pimentel ◽  
Martín Melo Munguía ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Acute Diarrhea ◽  
Mixed Infections ◽  
Reverse Transcription Pcr ◽  
Mexican Children ◽  
Human Rotavirus

Dual typing (VP4 and VP7) of rotavirus obtained from 257 Mexican children during three epidemiological seasons was performed by reverse transcription-PCR. The P1G1 genotype was the most prevalent (40%), followed by P1G3 (19%) and P2G2 (16%). Thirty-one specimens (12%) presented mixed infections, while some genotypes were not found. This is the first dual typing of isolates from diarrhea cases in Mexico.

scholarly journals Distribution of Human Rotavirus G Types Circulating in Paris, France, during the 1997–1998 Epidemic: High Prevalence of Type G4

1999 ◽  
Vol 37 (7) ◽  
pp. 2373-2375 ◽  
Author(s):  
Elyanne Gault ◽  
Roxane Chikhi-Brachet ◽  
Sandrine Delon ◽  
Nathalie Schnepf ◽  
Laurence Albiges ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Acute Diarrhea ◽  
Children's Hospital ◽  
Reverse Transcription Pcr ◽  
Human Rotavirus ◽  
High Prevalence ◽  
Children’S Hospital ◽  
Group A ◽  
Stool Specimens

Group A human rotavirus G genotypes were determined by means of reverse transcription-PCR in 170 stool specimens from children with acute diarrhea admitted to a Paris children’s hospital during a 1-year survey (1997 to 1998). The isolates all belonged to types G1 to G4, with type G4 predominating (60%).

scholarly journals Reference Human Rotavirus A Genome Sequence from a Previously Vaccinated Child with Diarrhea in Nigeria

2020 ◽  
Vol 9 (5) ◽  
Author(s):  
T. O. C. Faleye ◽  
U. E. George ◽  
C. Simsek ◽  
O. A. Arowolo ◽  
O. M. Adewumi ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Reverse Transcription ◽  
Rapid Test ◽  
Etiologic Agent ◽  
Rt Pcr ◽  
Rotavirus A ◽  
Reverse Transcription Pcr ◽  
Human Rotavirus ◽  
A Genome ◽  
Genotype Constellation

In 2018, a 26-month-old girl, fully vaccinated with Rotarix in 2016, presented with fever, diarrhea, and vomiting. A rapid test showed that her feces contained rotavirus A (RVA). VP7 reverse transcription-PCR (RT-PCR) and Illumina sequencing showed that a G1P[8] strain with a Wa-like genotype constellation was the etiologic agent. This is the first near-complete RVA genome sequence from Nigeria.

Evaluation of a multiplex real time reverse transcription PCR assay for the detection and quantitation of the most common human rotavirus genotypes

2012 ◽  
Vol 180 (1-2) ◽  
pp. 49-53 ◽  
Author(s):  
Christine Kottaridi ◽  
Aris T. Spathis ◽  
Chara Kleio Ntova ◽  
Vassiliki Papaevangelou ◽  
Petros Karakitsos
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Pcr Assay ◽  
Reverse Transcription Pcr ◽  
Human Rotavirus

scholarly journals Determination of Human Rotavirus VP6 Genogroups I and II by Reverse Transcription-PCR

2008 ◽  
Vol 46 (10) ◽  
pp. 3330-3337 ◽  
Author(s):  
Y.-P. Lin ◽  
C.-L. Kao ◽  
S.-Y. Chang ◽  
K. Taniguchi ◽  
P.-Y. Hung ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Reverse Transcription Pcr ◽  
Human Rotavirus ◽  
Rotavirus Vp6

scholarly journals G and P Genotyping of Rotavirus Strains Circulating in France over a Three-Year Period: Detection of G9 and P[6] Strains at Low Frequencies

2000 ◽  
Vol 38 (4) ◽  
pp. 1681-1683 ◽  
Author(s):  
F. Bon ◽  
C. Fromantin ◽  
S. Aho ◽  
P. Pothier ◽  
E. Kohli
Keyword(s):  
Reverse Transcription ◽  
Acute Diarrhea ◽  
Low Frequencies ◽  
Reverse Transcription Pcr ◽  
Period Detection

Rotavirus G and P types from 716 children with acute diarrhea in Dijon from 1995 to 1998 and throughout France during the winter of 1997-1998 were analyzed by reverse transcription-PCR. P[8],G1 predominated, followed by P[8],G4, which emerged during the last winter. G9 and P[6] strains were detected at low frequencies.

scholarly journals Genotype Profiles of Rotavirus Strains from Children in a Suburban Community in Guinea-Bissau, Western Africa

2000 ◽  
Vol 38 (1) ◽  
pp. 264-267
Author(s):  
Thea Kølsen Fischer ◽  
Hans Steinsland ◽  
Kåre Mølbak ◽  
Rui Ca ◽  
Jon R. Gentsch ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Mixed Infections ◽  
Group A Rotavirus ◽  
Guinea Bissau ◽  
Reverse Transcription Pcr ◽  
Western Africa ◽  
Suburban Community ◽  
Group A ◽  
Unusual Type

ABSTRACT The P (VP4) and G (VP7) genotypes of 167 group A rotavirus strains obtained during the period 1996 to 1998 from 149 children living in a suburban community in Guinea-Bissau, western Africa, were determined by the reverse transcription-PCR technique. A total of nine combinations including five different P types and five different G types were identified. The globally common genotype pairs P[8], G1; P[4], G2; P[8], G3 and P[8], G4 were underrepresented in this study area. We found a substantial year-to-year variation in the occurrence of the genotype combinations. In 1996 and 1997, P[6], G2 was the most frequent, whereas P[8], G1 was more common in 1998. The unusual type P[9], G3 and a few mixed infections were detected. Sixteen percent of the rotavirus-positive samples were nontypeable.

scholarly journals Implementation of an in-house real-time reverse transcription-PCR assay to detect the emerging SARS-CoV-2 N501Y variants

2021 ◽  
pp. 104868
Author(s):  
Marielle BEDOTTO ◽  
Pierre-Edouard FOURNIER ◽  
Linda HOUHAMDI ◽  
Philippe COLSON ◽  
Didier RAOULT
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Pcr Assay ◽  
Reverse Transcription Pcr
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