scholarly journals G and P Genotyping of Rotavirus Strains Circulating in France over a Three-Year Period: Detection of G9 and P[6] Strains at Low Frequencies

2000 ◽  
Vol 38 (4) ◽  
pp. 1681-1683 ◽  
Author(s):  
F. Bon ◽  
C. Fromantin ◽  
S. Aho ◽  
P. Pothier ◽  
E. Kohli
Keyword(s):  
Reverse Transcription ◽  
Acute Diarrhea ◽  
Low Frequencies ◽  
Reverse Transcription Pcr ◽  
Period Detection

Rotavirus G and P types from 716 children with acute diarrhea in Dijon from 1995 to 1998 and throughout France during the winter of 1997-1998 were analyzed by reverse transcription-PCR. P[8],G1 predominated, followed by P[8],G4, which emerged during the last winter. G9 and P[6] strains were detected at low frequencies.

scholarly journals VP4 and VP7 Genotyping by Reverse Transcription-PCR of Human Rotavirus in Mexican Children with Acute Diarrhea

2000 ◽  
Vol 38 (10) ◽  
pp. 3876-3878 ◽  
Author(s):  
Araceli Rodríguez Castillo ◽  
Andrés Velasco Villa ◽  
JoséErnesto Ramírez González ◽  
Elvira Mayén Pimentel ◽  
Martín Melo Munguía ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Acute Diarrhea ◽  
Mixed Infections ◽  
Reverse Transcription Pcr ◽  
Mexican Children ◽  
Human Rotavirus

Dual typing (VP4 and VP7) of rotavirus obtained from 257 Mexican children during three epidemiological seasons was performed by reverse transcription-PCR. The P1G1 genotype was the most prevalent (40%), followed by P1G3 (19%) and P2G2 (16%). Thirty-one specimens (12%) presented mixed infections, while some genotypes were not found. This is the first dual typing of isolates from diarrhea cases in Mexico.

scholarly journals Distribution of Human Rotavirus G Types Circulating in Paris, France, during the 1997–1998 Epidemic: High Prevalence of Type G4

1999 ◽  
Vol 37 (7) ◽  
pp. 2373-2375 ◽  
Author(s):  
Elyanne Gault ◽  
Roxane Chikhi-Brachet ◽  
Sandrine Delon ◽  
Nathalie Schnepf ◽  
Laurence Albiges ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Acute Diarrhea ◽  
Children's Hospital ◽  
Reverse Transcription Pcr ◽  
Human Rotavirus ◽  
High Prevalence ◽  
Children’S Hospital ◽  
Group A ◽  
Stool Specimens

Group A human rotavirus G genotypes were determined by means of reverse transcription-PCR in 170 stool specimens from children with acute diarrhea admitted to a Paris children’s hospital during a 1-year survey (1997 to 1998). The isolates all belonged to types G1 to G4, with type G4 predominating (60%).

scholarly journals Implementation of an in-house real-time reverse transcription-PCR assay to detect the emerging SARS-CoV-2 N501Y variants

2021 ◽  
pp. 104868
Author(s):  
Marielle BEDOTTO ◽  
Pierre-Edouard FOURNIER ◽  
Linda HOUHAMDI ◽  
Philippe COLSON ◽  
Didier RAOULT
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Pcr Assay ◽  
Reverse Transcription Pcr

scholarly journals Reverse Transcription-PCR Analysis of Bottled and Natural Mineral Waters for the Presence of Noroviruses

2003 ◽  
Vol 69 (11) ◽  
pp. 6541-6549 ◽  
Author(s):  
Gilbert Thierry Lamothe ◽  
Thierry Putallaz ◽  
Han Joosten ◽  
Joey D. Marugg
Keyword(s):  
Reverse Transcription ◽  
Membrane Filtration ◽  
Limit Of Detection ◽  
Mineral Waters ◽  
Pcr Analysis ◽  
Rna Sequences ◽  
Natural Mineral ◽  
Reverse Transcription Pcr ◽  
Viral Particles ◽  
Nonspecific Amplification

ABSTRACT A seminested reverse transcription-PCR method coupled to membrane filtration was optimized to investigate the presence of norovirus (NV) RNA sequences in bottled and natural mineral waters. The recovery of viral particles by filtration varied between 28 and 45%, while the limit of detection of the overall method ranged from 6 to 95 viral particles. The assay was broadly reactive, as shown by the successful detection of 27 different viral strains representing 12 common genotypes of NVs. A total of 718 bottled and natural mineral water samples were investigated, including 640 samples of finished, spring, and line products (mostly 1 to 1.5 liters), collected from 36 different water brands of various types and from diverse geographic origins over a 2-year period. In addition, 78 samples of larger volume (10 and 400 to 500 liters) and environmental swabs were investigated. From the 1,436 analyses that were performed for the detection of NVs belonging to genogroups I and II, 34 samples (2.44%) were presumptively positive by seminested RT-PCR. However, confirmation by DNA sequence analysis revealed that all presumptive positive results were either due to nonspecific amplification or to cross-contamination. In conclusion, these results do not provide any evidence for the presence of NV genome sequences in bottled waters.

Direct subtyping of HLA-B and -A genes by automated sequencing following reverse transcription-PCR

1996 ◽  
Vol 47 (1-2) ◽  
pp. 115
Author(s):  
AJ Knipper ◽  
J Enczmann ◽  
P Hakenberg ◽  
G Kögler ◽  
P Wernet
Keyword(s):  
Reverse Transcription ◽  
Reverse Transcription Pcr

scholarly journals Aromatic Hydroxylation of Indan by o-Xylene-Degrading Rhodococcus sp. Strain DK17

2009 ◽  
Vol 76 (1) ◽  
pp. 375-377 ◽  
Author(s):  
Dockyu Kim ◽  
Choong Hwan Lee ◽  
Jung Nam Choi ◽  
Ki Young Choi ◽  
Gerben J. Zylstra ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Ring Cleavage ◽  
Growth Substrate ◽  
Aromatic Moiety ◽  
Reverse Transcription Pcr ◽  
Aromatic Hydroxylation ◽  
Rhodococcus Sp

ABSTRACT The metabolically versatile Rhodococcus sp. strain DK17 utilizes indan as a growth substrate via the o-xylene pathway. Metabolite and reverse transcription-PCR analyses indicate that o-xylene dioxygenase hydroxylates indan at the 4,5 position of the aromatic moiety to form cis-indan-4,5-dihydrodiol, which is dehydrogenated to 4,5-indandiol by a dehydrogenase. 4,5-Indandiol undergoes ring cleavage by a meta-cleavage dioxygenase.

scholarly journals Increased Expression of Two Multidrug Transporter-Like Genes Is Associated with Ethidium Bromide and Ciprofloxacin Resistance in Mycoplasma hominis

2005 ◽  
Vol 49 (1) ◽  
pp. 421-424 ◽  
Author(s):  
S. Raherison ◽  
P. Gonzalez ◽  
H. Renaudin ◽  
A. Charron ◽  
C. Bébéar ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Ethidium Bromide ◽  
Reverse Transcription ◽  
Mycoplasma Hominis ◽  
Atp Binding ◽  
Multidrug Transporter ◽  
Reverse Transcription Pcr ◽  
Atp Binding Cassette Transporters ◽  
Resistant Strains ◽  
Ciprofloxacin Resistance

ABSTRACT Two genes, md1 and md2, coding for multidrug resistance ATP-binding cassette transporters were identified in Mycoplasma hominis PG21. Expression of these two genes, quantified by quantitative competitive reverse transcription-PCR, was significantly increased in ethidium bromide-resistant strains of M. hominis compared to that in M. hominis PG21.

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