scholarly journals Nosocomial Transmission of Echovirus 30: Molecular Evidence by Phylogenetic Analysis of the VP1 Encoding Sequence

2000 ◽  
Vol 38 (8) ◽  
pp. 2889-2892 ◽  
Author(s):  
Jean-Luc Bailly ◽  
Aline Béguet ◽  
Martine Chambon ◽  
Cécile Henquell ◽  
Hélène Peigue-Lafeuille
Keyword(s):  
Phylogenetic Analysis ◽  
Phylogenetic Tree ◽  
Aseptic Meningitis ◽  
Index Patient ◽  
Nosocomial Transmission ◽  
Molecular Evidence ◽  
Enterovirus Infection ◽  
Monophyletic Cluster ◽  
Echovirus 30 ◽  
Secondary Infections

We investigated six cases of enterovirus infection in a neonatal unit. The index patient, a 5-day-old boy, was admitted with aseptic meningitis due to echovirus 30 (E30). Secondary infections with E30 occurred in five babies. Comparison of the complete VP1 sequences showed that the isolates recovered from the index patient and his mother were closely related to those recovered from the five babies with secondary infections, demonstrating a nosocomial transmission of the virus. In the phylogenetic tree reconstructed from the VP1 sequences, the isolates formed a monophyletic cluster related to an E30 strain collected in June 1997 during an outbreak of aseptic meningitis.

scholarly journals Hepatitis C in a Hemodialysis Unit: Molecular Evidence for Nosocomial Transmission

1998 ◽  
Vol 36 (10) ◽  
pp. 3040-3043 ◽  
Author(s):  
S. Le Pogam ◽  
D. Le Chapois ◽  
R. Christen ◽  
F. Dubois ◽  
F. Barin ◽  
...  
Keyword(s):  
Hepatitis C ◽  
Phylogenetic Analyses ◽  
Index Patient ◽  
Molecular Evidence ◽  
Virus Genotype ◽  
Genotype 4 ◽  
Source Of Infection ◽  
Secondary Infections ◽  
Hemodialysis Unit ◽  
Virus Strains

This study reports four cases of transmission of the rare hepatitis C virus genotype 4 which occurred in a hemodialysis unit and originated from a single source of infection. Phylogenetic analyses performed with the NS5b domain showed that all four patients with secondary infections were infected with virus strains very similar to that of the index patient. Cross infections probably resulted from breaches in safety precautions and lack of dialysis machine sterilization.

scholarly journals Two new Chrysosporium (Onygenaceae, Onygenales) from China

Phytotaxa ◽  
2016 ◽  
Vol 270 (3) ◽  
pp. 210 ◽  
Author(s):  
YAN-WEI ZHANG ◽  
WANHAO CHEN ◽  
GUIPING ZENG ◽  
XIAO ZOU ◽  
TINGCHI WEN ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Phylogenetic Tree ◽  
Soil Samples ◽  
The Other ◽  
Guizhou Province ◽  
Molecular Evidence ◽  
5.8S Rdna ◽  
Rdna Sequences ◽  
Fungal Isolates

Fungal isolates GZUIFR-EM14.2002 and GZUIFR-EM66601 were respectively isolated from Chinese soil samples under the snake skin in Guizhou Province and from the soil samples under the feathers in Hubei Province, China. Morphological and molecular evidence support both isolates as new species of Chrysosporium. Phylogenetic analysis based on ITS-5.8S rDNA sequences grouped GZUIFR-EM14.2002 together with C. lucknowense and C. mephiticum. GZUIFR-EM14.2002, which could be distinguished from the latter two species by the presence of abundant intercalary conidia, was named C. guizhouense sp. nov.  In the phylogenetic tree, GZUIFR-EM66601 was most closely related to C. submersum and C. siglerae, GZUIFR-EM66601 differed from the other two species in having small obovate to ellipsoidal conidia and no intercalary conidia; this strain was designated as C. hubeiense sp. nov. Holotypes and their isolates had been deposited in GZAC, Guiyang, Guizhou Province, China.

scholarly journals Molecular diagnosis of echovirus 30 as the etiological agent in an outbreak of aseptic meningitis in Panama: May – June 2008

2012 ◽  
Vol 6 (12) ◽  
pp. 836-841 ◽  
Author(s):  
Alexander A Martinez ◽  
Juan Castillo ◽  
Mirla C Sanchez ◽  
Yamitzel Zaldivar ◽  
Yaxelis Mendoza ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Aseptic Meningitis ◽  
Rna Extraction ◽  
Molecular Techniques ◽  
Etiological Agent ◽  
Rt Pcr ◽  
Viral Pathogens ◽  
Echovirus 30 ◽  
Specific Pcr ◽  
Health Studies

Introduction: Aseptic meningitis outbreaks are commonly caused by viral pathogens with enterovirus a common etiological agent. Between May and June of 2008, an outbreak of 173 cases of aseptic meningitis occurred in the Chiriqui Province of Panama. Molecular techniques were used to identify the etiological agent. Methodology: Cerebrospinal fluid (CSF) samples from 75 patients were received at the Gorgas Memorial Institute for Health Studies.  RNA extraction and one-step RT-PCR were performed on each sample to determine the presence of enterovirus.  Thirty-four samples which were positive for enterovirus were subject to group-specific PCR, sequencing, and phylogenetic analysis to identify the etiological agent of the outbreak. Results: The CSF of 58 subjects was found positive for the enterovirus family using RT-PCR. Thirty-four samples were found to belong to the enterovirus B group. Phylogenetic analysis of four successfully sequenced samples revealed echovirus 30 as the etiological agent. Conclusion: Echovirus 30 is reported as the likely cause of an outbreak of aseptic meningitis in Panama, the first since the 1980s.

Molecular Evidence for Nosocomial Transmission of Human Immunodeficiency Virus from a Surgeon to One of His Patients

1998 ◽  
Vol 72 (5) ◽  
pp. 4537-4540 ◽  
Author(s):  
Alain Blanchard ◽  
Stéphane Ferris ◽  
Sophie Chamaret ◽  
Denise Guétard ◽  
Luc Montagnier
Keyword(s):  
Human Immunodeficiency Virus ◽  
Viral Genome ◽  
Pcr Amplification ◽  
Nosocomial Transmission ◽  
Molecular Evidence ◽  
Immunodeficiency Virus ◽  
Reference Sequences ◽  
Hiv Type 1 ◽  
Viral Sequences

ABSTRACT We have investigated the molecular evidence in favor of the transmission of human immunodeficiency virus (HIV) from an HIV-infected surgeon to one of his patients. After PCR amplification, theenv and gag sequences from the viral genome were cloned and sequenced. Phylogenetic analysis revealed that the viral sequences derived from the surgeon and his patient are closely related, which strongly suggests that nosocomial transmission occurred. In addition, these viral sequences belong to group M of HIV type 1 but are divergent from the reference sequences of the known subtypes.

A REVISED GENERIC SYNOPSIS OF HYACINTHACEAE IN SUB-SAHARAN AFRICA, BASED ON MOLECULAR EVIDENCE, INCLUDING NEW COMBINATIONS AND THE NEW TRIBE PSEUDOPROSPEREAE

2003 ◽  
Vol 60 (3) ◽  
pp. 533-568 ◽  
Author(s):  
J. C. MANNING ◽  
P. GOLDBLATT ◽  
M. F. FAY
Keyword(s):  
Phylogenetic Analysis ◽  
Sub Saharan Africa ◽  
Molecular Evidence ◽  
New Combinations ◽  
Generic Level ◽  
The Family ◽  
Molecular Phylogenetic ◽  
Single Genus ◽  
New Tribe ◽  
Sub Saharan

A revised generic synopsis of sub-Saharan Hyacinthaceae is presented, based on a molecular phylogenetic analysis of the family. Generic rank is accorded only to reciprocally monophyletic clades that can be distinguished by recognizable morphological discontinuities, thereby permitting an appropriate generic assignment of species not included in the analysis. Three subfamilies are recognized within the region. Subfamily Ornithogaloideae, characterized by flattened or angular seeds with tightly adhering testa, is considered to include the single genus Ornithogalum, which is expanded to include the genera Albuca, Dipcadi, Galtonia, Neopatersonia and Pseudogaltonia. Recognizing any of these segregates at generic level renders the genus Ornithogalum polyphyletic, while subdivision of Ornithogalum into smaller, morphologically distinguishable segregates in order to preserve the monophyly of each is not possible. Subfamily Urgineoideae, characterized by flattened or winged seeds with brittle, loosely adhering testa, comprises the two mainland African genera Bowiea and Drimia. The latter is well circumscribed by its deciduous, short-lived perianth and includes the previously recognized genera Litanthus, Rhadamanthus, Schizobasis and Tenicroa. The monotypic Madagascan Igidia is provisionally included in the subfamily as a third genus on the basis of its seeds, pending molecular confirmation of its relationships. Subfamily Hyacinthoideae resolves into three clades, distinguished as tribes Hyacintheae (strictly northern hemisphere and not treated further), Massonieae and Pseudoprospereae tribus nov. Full descriptions and a key to their identification are provided for all genera. New combinations reflecting the generic circumscriptions adopted here are made for most African and all Indian and Madagascan species.

Rhizocarpon smaragdulum, a new monosporic yellow-thalline species and some additional species of the genus Rhizocarpon from the Altai Mountains (Siberia)

2017 ◽  
Vol 49 (5) ◽  
pp. 457-466 ◽  
Author(s):  
Evgeny A. DAVYDOV ◽  
Lidia S. YAKOVCHENKO
Keyword(s):  
Phylogenetic Analysis ◽  
Phylogenetic Tree ◽  
Altai Mountains ◽  
Additional Species ◽  
Sister Relationship

AbstractRhizocarpon smaragdulum Davydov & Yakovchenko sp. nov. is described and a phylogenetic analysis (ITS, mtSSU) is presented, confirming its distinctiveness and indicating a sister relationship with R. suomiense and R. subgeminatum. The species is unique among yellow Rhizocarpon species in having a single hyaline ascospore per ascus. The phylogenetic tree suggests that the number of ascospores per ascus has been reduced in Rhizocarpon more than once during the course of its evolution. Two new distributional records are also reported: Rhizocarpon atroflavescens is new for Siberia and R. norvegicum is new for the Altai Mountains. Rhizocarpon norvegicum in this region grows on rocks and is also lichenicolous on Acarospora bullata.

scholarly journals Echovirus 30 associated with cases of aseptic meningitis in state of Pará, Northern Brazil

2009 ◽  
Vol 104 (3) ◽  
pp. 444-450 ◽  
Author(s):  
Ceyla Maria Oeiras de Castro ◽  
Darleise S Oliveira ◽  
Olinda Macedo ◽  
Maria José L Lima ◽  
Marquete B Santana ◽  
...  
Keyword(s):  
Aseptic Meningitis ◽  
Echovirus 30 ◽  
Northern Brazil

scholarly journals Phylogenetic Analysis of Kobuvirus and Astrovirus From Korean Wild Boars: 2016-2018

2021 ◽  
Author(s):  
Jihye Shin ◽  
SeEun Choe ◽  
Bang-Hun Hyun ◽  
Dong-Jun An
Keyword(s):  
Phylogenetic Analysis ◽  
Phylogenetic Tree ◽  
Porcine Kobuvirus ◽  
Phylogenetic Tree Analysis ◽  
Tree Analysis ◽  
Wild Boars ◽  
Porcine Astrovirus ◽  
The One

Abstract The prevalence of porcine kobuvirus (PKoV) and porcine astrovirus (PAstV) in 845 Korean wild boars (KWB) during 2016-2018 were 28.0% and 10.6%, respectively, and co-infection of two viruses showed 5.1%. Phylogenetic tree analysis also revealed that 236 PKoVs from KWB were divided to diverse lineages within Aichivirus C group but the one strain (WKoV16CN-8627) was included the same cluster with bovine kobuvirus (Achivirus B). Eighty-nine PAstVs from KWB was belonged predominantly to lineage PAstV4 and only one strain (WAst17JN-10931) included novel to lineage PAstV2. Two viruses are epidemic more in young (≤ 12 months) than in old pigs (> 12 months).

scholarly journals Four new corticioid species in Trechisporales (Basidiomycota) from East Asia and notes on phylogeny of the order

MycoKeys ◽  
2019 ◽  
Vol 48 ◽  
pp. 97-113
Author(s):  
Shi-Liang Liu ◽  
Hai-Xia Ma ◽  
Shuang-Hui He ◽  
Yu-Cheng Dai
Keyword(s):  
New Species ◽  
Phylogenetic Tree ◽  
East Asia ◽  
Molecular Evidence ◽  
Family Level ◽  
First Time

Four new species in Trechisporales from East Asia, Dextrinocystiscalamicola, Subulicystidiumacerosum, S.tropicum and Tubuliciumbambusicola, are described and illustrated, based on morphological and molecular evidence. The phylogeny of Trechisporales was inferred from a combined dataset of ITS-nrLSU sequences. In the phylogenetic tree, Sistotremastrum formed a family-level clade of its own, sister to the Hydnodontaceae clade formed by all other genera. Dextrinocystis, is for the first time, confirmed as a member of Hydnodontaceae. A key to all the accepted genera in Trechisporales is given.

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