scholarly journals Simultaneous Detection of Anaplasma marginale and a New Ehrlichia Species Closely Related to Ehrlichia chaffeensis by Sequence Analyses of 16S Ribosomal DNA in Boophilus microplus Ticks from Tibet

2002 ◽  
Vol 40 (9) ◽  
pp. 3286-3290 ◽  
Author(s):  
B. Wen ◽  
R. Jian ◽  
Y. Zhang ◽  
R. Chen
Keyword(s):  
Ribosomal Dna ◽  
Simultaneous Detection ◽  
Anaplasma Marginale ◽  
Boophilus Microplus ◽  
Ehrlichia Chaffeensis ◽  
Sequence Analyses ◽  
16S Ribosomal Dna ◽  
Boophilus Microplus Ticks

scholarly journals First Molecular Characterization of Anaplasma marginale in Cattle and Rhipicephalus (Boophilus) microplus Ticks in Cebu, Philippines

2013 ◽  
Vol 75 (1) ◽  
pp. 27-36 ◽  
Author(s):  
Adrian Patalinghug YBAÑEZ ◽  
Thillaiampalam SIVAKUMAR ◽  
Rochelle Haidee Daclan YBAÑEZ ◽  
Jowarren Catingan RATILLA ◽  
Zandro Obligado PEREZ ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Anaplasma Marginale ◽  
Boophilus Microplus ◽  
Boophilus Microplus Ticks

scholarly journals Simultaneous Discrimination between 15 Fish Pathogens by Using 16S Ribosomal DNA PCR and DNA Microarrays

2004 ◽  
Vol 70 (7) ◽  
pp. 4216-4221 ◽  
Author(s):  
Adelaide E. Warsen ◽  
Melissa J. Krug ◽  
Stacey LaFrentz ◽  
Danielle R. Stanek ◽  
Frank J. Loge ◽  
...  
Keyword(s):  
16S Rdna ◽  
Ribosomal Dna ◽  
Bacterial Species ◽  
Simultaneous Detection ◽  
Detection Sensitivity ◽  
Fish Pathogens ◽  
Universal Primer ◽  
16S Ribosomal Dna ◽  
Glass Slides ◽  
16S Rdna Pcr

ABSTRACT We developed a DNA microarray suitable for simultaneous detection and discrimination between multiple bacterial species based on 16S ribosomal DNA (rDNA) polymorphisms using glass slides. Microarray probes (22- to 31-mer oligonucleotides) were spotted onto Teflon-masked, epoxy-silane-derivatized glass slides using a robotic arrayer. PCR products (ca. 199 bp) were generated using biotinylated, universal primer sequences, and these products were hybridized overnight (55°C) to the microarray. Targets that annealed to microarray probes were detected using a combination of Tyramide Signal Amplification and Alexa Fluor 546. This methodology permitted 100% specificity for detection of 18 microbes, 15 of which were fish pathogens. With universal 16S rDNA PCR (limited to 28 cycles), detection sensitivity for purified control DNA was equivalent to <150 genomes (675 fg), and this sensitivity was not adversely impacted either by the presence of competing bacterial DNA (1.1 × 106 genomes; 5 ng) or by the addition of up to 500 ng of fish DNA. Consequently, coupling 16S rDNA PCR with a microarray detector appears suitable for diagnostic detection and surveillance for commercially important fish pathogens.

Lack of infectivity of a Brazilian Anaplasma marginale isolate for Boophilus microplus ticks

2005 ◽  
Vol 128 (3-4) ◽  
pp. 325-331 ◽  
Author(s):  
Patrícia M. Gonçalves Ruiz ◽  
Lygia M. Friche Passos ◽  
Múcio F. Barbosa Ribeiro
Keyword(s):  
Anaplasma Marginale ◽  
Boophilus Microplus ◽  
Boophilus Microplus Ticks

scholarly journals Bacterial Communities Associated with Host-Adapted Populations of Pea Aphids Revealed by Deep Sequencing of 16S Ribosomal DNA

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0120664 ◽  
Author(s):  
Jean-Pierre Gauthier ◽  
Yannick Outreman ◽  
Lucie Mieuzet ◽  
Jean-Christophe Simon
Keyword(s):  
Deep Sequencing ◽  
Ribosomal Dna ◽  
Bacterial Communities ◽  
16S Ribosomal Dna ◽  
Pea Aphids
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