scholarly journals Reduction of variation in results of rheumatoid factor tests by use of a serum reference preparation

1977 ◽  
Vol 5 (1) ◽  
pp. 42-45
Author(s):  
R N Taylor ◽  
K M Fulford ◽  
W L Jones
Keyword(s):  
Disease Control ◽  
Rheumatoid Factor ◽  
Proficiency Testing ◽  
Test Results ◽  
Testing Program ◽  
Product Standardization ◽  
Reference Preparation ◽  
Reference Serum

Standaridizing test results for rheumatoid factor by comparing results obtained for an unknown with results obtained for a serum reference preparation decreased variance between laboratories, as measured in the Center for Disease Control proficiency testing program, by 77%. The amount of improvement was also estimated by the type of test and by the manufacturer's product. Standardization resulted in an increase in the number of reported results that were within a twofold dilution of the median value. The percentage increased from 50.3 to 93.7% for the slide tests and from 78.1 to 91.2% fro the tube tests. Decrease in variance by manufacturer's product ranged from 94 to 27%. The study demonstrated that adopting a reference serum standard could substantially improve the comparability of rheumatoid factor test results and that proficiency testing programs can be used to estimate improvement which could be expected as a result of standardization.

scholarly journals Reference preparation to standardize results of serological tests for rheumatoid factor

1978 ◽  
Vol 7 (5) ◽  
pp. 434-441
Author(s):  
K M Fulford ◽  
R N Taylor ◽  
V A Przybyszewski
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Proficiency Testing ◽  
Factor Level ◽  
Serological Tests ◽  
Laboratory Results ◽  
Reference Preparation ◽  
Reference Serum ◽  
Comparability Of Results ◽  
Latex Test

The variance of between-laboratory results for rheumatoid factor tests was reduced through the use of a reference serum preparation. Two separate proficiency testing surveys have shown reduction of 58 to 81% in variance with samples of differing levels and composition. Reduction of variance was seen in data from laboratories in which the same methods and reagents were used, but greater reduction in variance was seen in results from laboratories using different methods and reagents. This study demonstrates that comparability of results of rheumatoid factor tests can be significantly improved through the use of a standard reference serum preparation and that confidence in such results can therefore be increased. A serum rheumatoid factor level of approximately 50 IU/ml is equivalent to a titer of 160 in the Singer-Plotz latex test, which has traditionally been accepted as evidence of rheumatoid arthritis.

Center for Disease Control Diagnostic Immunology Proficiency Testing Program results for 1977

1978 ◽  
Vol 8 (4) ◽  
pp. 388-395
Author(s):  
R N Taylor ◽  
K M Fulford ◽  
V A Przybyszewski ◽  
V Pope
Keyword(s):  
Hepatitis B ◽  
Disease Control ◽  
Rheumatoid Factor ◽  
Proficiency Testing ◽  
Hepatitis B Surface Antigen ◽  
Testing Program ◽  
Specific Proteins ◽  
Syphilis Serology ◽  
Testing Protocols ◽  
Yearly Data

A summary of the yearly data accumulated and analyzed in the diagnostic immunology portion of the Center for Disease Control 1977 Proficiency Testing Program provides information on the trends in testing protocols from over 900 participating laboratories. One hundred and fourteen specimens prepared by the Center for the Disease Control were distributed quarterly or in special surveys. The specimens chosen provided feedback from a broad range of tests commonly performed in diagnostic immunology laboratories and included those for rubella antibodies, hepatitis B surface antigen, bacterial antibodies, rheumatoid factor, immunoglobulins and other serum-specific proteins, syphilis serology, and carcinoembryonic antigen. This summary provides an overview of the trends observed since last year's report.

Results of the Center for Disease Control Proficiency Testing Program for the detection of hepatitis B surface antigen

1976 ◽  
Vol 4 (1) ◽  
pp. 32-39
Author(s):  
R N Taylor ◽  
K M Fulford
Keyword(s):  
Hepatitis B ◽  
Disease Control ◽  
Proficiency Testing ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Latex Agglutination ◽  
Lower Sensitivity ◽  
Passive Hemagglutination ◽  
Testing Program ◽  
Efficiency And Effectiveness

The changes in the types and numbers of tests used by participants in the Center for Disease Control Proficiency Testing Program for the detection of hepatitis B surface antigen from the beginning of the program in 1971 until October 1975 are analyzed; the implications of these changes are discussed. Changes in the use of agar gel diffusion, rheophoresis, counterelectrophoresis, complement fixation, reverse passive latex agglutination, radioimmunoassay, and reverse passive hemagglutination tests are reviewed. The performance of the participants for 1975 is reported, and factors related to performance (type or combinations of tests used, procedures used to confirm specificity, etc.) are discussed. The increase in the use of third-generation tests in place of tests with lower sensitivity and the decrease in the use of multiple tests undoubtedly represent increased efficiency and effectiveness.

Center for disease control diagnostic immunology proficiency testing program results for 1976

1977 ◽  
Vol 6 (3) ◽  
pp. 224-232
Author(s):  
R N Taylor ◽  
K M Fulford ◽  
A Przybyszewski ◽  
V Pope
Keyword(s):  
Disease Control ◽  
Proficiency Testing ◽  
Hepatitis B Surface Antigen ◽  
Testing Program ◽  
Proficiency Levels ◽  
Clinical Laboratories ◽  
Individual Laboratory ◽  
Specific Proteins ◽  
Overall Performance ◽  
Testing Protocols

Over 900 laboratories participated in the Diagnostic Immunology portion of the 1976 Proficiency Testing Program, which was provided by the Center of Disease Control under the authority of the Clinical Laboratories Improvement Act of 1967. One hundred specimens prepared by the Center for Disease Control for analysis were distributed on a quarterly schedule or in special surveys. Feedback from participating laboratories included over 37,500 qualitative and 33,000 quantitative responses, which were analyzed to determine individual laboratory proficiency levels. In addition, information supplied by participants in each survey helped to delineate trends in testing protocols. The specimens chosen for analysis called for a broad range of tests commonly performed in diagnostic immunology laboratories, including those for rubella antibodies, hepatitis B surface antigen, bacterial antibodies, rheumatoid factor, immunoglobulins and other serum-specific proteins, and carcinoembryonic antigen. A summary of the data analysis is provided so that the laboratories can improve their overall performance levels.

scholarly journals Use of standard samples for qualification inspections of testing laboratory for analysis safety of meat and meat products

2021 ◽  
pp. 45-53
Author(s):  
L. K. Baybolova ◽  
A. K. Khaimuldinova ◽  
Zh. B. Asirzhanova
Keyword(s):  
Proficiency Testing ◽  
Meat Products ◽  
Test Preparation ◽  
Test Results ◽  
Standard Samples ◽  
Comparative Testing ◽  
Testing Laboratory ◽  
Testing Program ◽  
Testing Laboratories ◽  
Qualification Testing

This article provides insights into qualification testing, interlaboratory comparative testing, and qualification testing programs in testing laboratories that analyze the safety of meat and meat products. Samples with codes OK-MB- 21 were used as a model for interlaboratory comparisons. The uniformity and stability of the CC code was assessed in accordance with GOST R 50779.60-2017 “Statistical methods. Application in proficiency testing through interlaboratory testing ”. Seven laboratories participated in the proficiency testing program. The test preparation procedure was described and the test results determined whether each sample tested was satisfactory or questionable. Participants with unsatisfactory results are encouraged to identify the causes of the alarms, take corrective actions and document them, and identify and eliminate the causes of the unsatisfactory results.

scholarly journals Center for Disease Control Diagnostic Immunology Proficiency Testing Program results for 1978.

1979 ◽  
Vol 10 (6) ◽  
pp. 805-814 ◽  
Author(s):  
R N Taylor ◽  
K M Fulford ◽  
V A Przybyszewski ◽  
V Pope
Keyword(s):  
Disease Control ◽  
Proficiency Testing ◽  
Testing Program

Turbidimetry of rheumatoid factor in serum with a centrifugal analyzer.

1986 ◽  
Vol 32 (1) ◽  
pp. 124-129 ◽  
Author(s):  
L Borque ◽  
M Yago ◽  
C Mar ◽  
C Rodriguez
Keyword(s):  
Rheumatoid Arthritis ◽  
Human Serum ◽  
Rheumatoid Factor ◽  
Test Results ◽  
Serum Samples ◽  
Human Igg ◽  
Standard Curve ◽  
Igm Rheumatoid Factor ◽  
Triglyceride Concentration ◽  
Reference Serum

Abstract We describe the simple, rapid turbidimetry of IgM rheumatoid factor in human serum by use of the Cobas-Bio centrifugal analyzer. Heat-aggregated human IgG is used as the antigen. The immunoturbidimetric reaction is monitored at 340 nm for 300 s, and the changes in absorbance after the antigen is added are used to prepare the standard curve. Test results are calculated from the stored curve and reported in int. units/mL, based on comparison with the WHO reference serum for rheumatoid factor. There is no interference from bilirubin (up to 340 mumol/L) or hemoglobin (up to 5600 mg/L). Serum samples with a triglyceride concentration greater than 2.20 mmol/L must be cleared of lipids before analysis. The standard curve is linear from 30 to 500 int. units/mL. Precision, accuracy, linearity, and sensitivity are quite acceptable. The CV was generally less than 5% for different concentrations of rheumatoid factor. Results agree well with those by a rate-nephelometric procedure on the Beckman ICS system (rs = 0.932). However, both correlate poorly with a modified classical Waaler-Rose test. Of 47 patients with rheumatoid arthritis, 34 had IgM rheumatoid factor in their serum, but the measured value did not reflect the activity of the disease.

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