Center for Disease Control Diagnostic Immunology Proficiency Testing Program results for 1977

1978 ◽  
Vol 8 (4) ◽  
pp. 388-395
Author(s):  
R N Taylor ◽  
K M Fulford ◽  
V A Przybyszewski ◽  
V Pope
Keyword(s):  
Hepatitis B ◽  
Disease Control ◽  
Rheumatoid Factor ◽  
Proficiency Testing ◽  
Hepatitis B Surface Antigen ◽  
Testing Program ◽  
Specific Proteins ◽  
Syphilis Serology ◽  
Testing Protocols ◽  
Yearly Data

A summary of the yearly data accumulated and analyzed in the diagnostic immunology portion of the Center for Disease Control 1977 Proficiency Testing Program provides information on the trends in testing protocols from over 900 participating laboratories. One hundred and fourteen specimens prepared by the Center for the Disease Control were distributed quarterly or in special surveys. The specimens chosen provided feedback from a broad range of tests commonly performed in diagnostic immunology laboratories and included those for rubella antibodies, hepatitis B surface antigen, bacterial antibodies, rheumatoid factor, immunoglobulins and other serum-specific proteins, syphilis serology, and carcinoembryonic antigen. This summary provides an overview of the trends observed since last year's report.

Center for disease control diagnostic immunology proficiency testing program results for 1976

1977 ◽  
Vol 6 (3) ◽  
pp. 224-232
Author(s):  
R N Taylor ◽  
K M Fulford ◽  
A Przybyszewski ◽  
V Pope
Keyword(s):  
Disease Control ◽  
Proficiency Testing ◽  
Hepatitis B Surface Antigen ◽  
Testing Program ◽  
Proficiency Levels ◽  
Clinical Laboratories ◽  
Individual Laboratory ◽  
Specific Proteins ◽  
Overall Performance ◽  
Testing Protocols

Over 900 laboratories participated in the Diagnostic Immunology portion of the 1976 Proficiency Testing Program, which was provided by the Center of Disease Control under the authority of the Clinical Laboratories Improvement Act of 1967. One hundred specimens prepared by the Center for Disease Control for analysis were distributed on a quarterly schedule or in special surveys. Feedback from participating laboratories included over 37,500 qualitative and 33,000 quantitative responses, which were analyzed to determine individual laboratory proficiency levels. In addition, information supplied by participants in each survey helped to delineate trends in testing protocols. The specimens chosen for analysis called for a broad range of tests commonly performed in diagnostic immunology laboratories, including those for rubella antibodies, hepatitis B surface antigen, bacterial antibodies, rheumatoid factor, immunoglobulins and other serum-specific proteins, and carcinoembryonic antigen. A summary of the data analysis is provided so that the laboratories can improve their overall performance levels.

Results of the Center for Disease Control Proficiency Testing Program for the detection of hepatitis B surface antigen

1976 ◽  
Vol 4 (1) ◽  
pp. 32-39
Author(s):  
R N Taylor ◽  
K M Fulford
Keyword(s):  
Hepatitis B ◽  
Disease Control ◽  
Proficiency Testing ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Latex Agglutination ◽  
Lower Sensitivity ◽  
Passive Hemagglutination ◽  
Testing Program ◽  
Efficiency And Effectiveness

The changes in the types and numbers of tests used by participants in the Center for Disease Control Proficiency Testing Program for the detection of hepatitis B surface antigen from the beginning of the program in 1971 until October 1975 are analyzed; the implications of these changes are discussed. Changes in the use of agar gel diffusion, rheophoresis, counterelectrophoresis, complement fixation, reverse passive latex agglutination, radioimmunoassay, and reverse passive hemagglutination tests are reviewed. The performance of the participants for 1975 is reported, and factors related to performance (type or combinations of tests used, procedures used to confirm specificity, etc.) are discussed. The increase in the use of third-generation tests in place of tests with lower sensitivity and the decrease in the use of multiple tests undoubtedly represent increased efficiency and effectiveness.

scholarly journals Reduction of variation in results of rheumatoid factor tests by use of a serum reference preparation

1977 ◽  
Vol 5 (1) ◽  
pp. 42-45
Author(s):  
R N Taylor ◽  
K M Fulford ◽  
W L Jones
Keyword(s):  
Disease Control ◽  
Rheumatoid Factor ◽  
Proficiency Testing ◽  
Test Results ◽  
Testing Program ◽  
Product Standardization ◽  
Reference Preparation ◽  
Reference Serum

Standaridizing test results for rheumatoid factor by comparing results obtained for an unknown with results obtained for a serum reference preparation decreased variance between laboratories, as measured in the Center for Disease Control proficiency testing program, by 77%. The amount of improvement was also estimated by the type of test and by the manufacturer's product. Standardization resulted in an increase in the number of reported results that were within a twofold dilution of the median value. The percentage increased from 50.3 to 93.7% for the slide tests and from 78.1 to 91.2% fro the tube tests. Decrease in variance by manufacturer's product ranged from 94 to 27%. The study demonstrated that adopting a reference serum standard could substantially improve the comparability of rheumatoid factor test results and that proficiency testing programs can be used to estimate improvement which could be expected as a result of standardization.

scholarly journals Center for Disease Control Diagnostic Immunology Proficiency Testing Program results for 1978.

1979 ◽  
Vol 10 (6) ◽  
pp. 805-814 ◽  
Author(s):  
R N Taylor ◽  
K M Fulford ◽  
V A Przybyszewski ◽  
V Pope
Keyword(s):  
Disease Control ◽  
Proficiency Testing ◽  
Testing Program

scholarly journals Interference in the indirect antiglobulin test and direct antiglobulin test from rheumatoid factor

2019 ◽  
Vol 48 (3) ◽  
pp. 030006051989238
Author(s):  
Lei Xu ◽  
Hao Li ◽  
Sishi Yang ◽  
Wenjuan Zeng ◽  
Shan Gan ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Rheumatoid Factor ◽  
Blood Cells ◽  
Hepatitis B Surface Antigen ◽  
Direct Antiglobulin Test ◽  
Interference Effects ◽  
Indirect Antiglobulin Test ◽  
Antiglobulin Test ◽  
One Step ◽  
The One

Background The indirect antiglobulin test (IAT) and direct antiglobulin test (DAT) have been used as common tests for transfusion. Recently, we have found that in addition to causing false increases, rheumatoid factor (RF) can also cause false decreases in immunoassays for hepatitis B surface antigen and B-type natriuretic peptide. However, it remains unclear whether RF also interferes with the IAT and DAT. Methods IAT models were produced by mixing IAT-positive plasma and RF-positive plasma, then one-step and two-step IATs were adopted for detection. DAT models were produced by mixing DAT-positive red blood cells (RBCs) and RF-positive plasma, followed by detection with the DAT. The DAT models were diluted using the same RF-positive plasma, and the DAT was performed again. Results The rate of decrease of the two-step IAT (40.63%) was significantly higher than that of the one-step IAT (31.51%). Both the rate of decrease (76.67%) and increase (16.67%) of the results of the 60 DAT models were significantly higher than those of the IAT models after two-fold dilution. Conclusions The RF can lead to both false decreases and false increases in IAT and DAT. And the interference effects are related to the RF content relative to the IgG-sensitized RBCs.

scholarly journals Negative interference by rheumatoid factor in alpha-fetoprotein chemiluminescent microparticle immunoassay

2016 ◽  
Vol 54 (1) ◽  
pp. 55-59 ◽  
Author(s):  
Hui Wang ◽  
Xiaohui Bi ◽  
Lei Xu ◽  
Yirong Li
Keyword(s):  
Hepatitis B ◽  
Rheumatoid Factor ◽  
Hepatitis B Surface Antigen ◽  
Alpha Fetoprotein ◽  
Serum Samples ◽  
Negative Interference ◽  
Positive Serum ◽  
Serum Alpha Fetoprotein ◽  
Chemiluminescent Microparticle Immunoassay ◽  
Paramagnetic Microparticles

Background Rheumatoid factor causes positive interference in multiple immunoassays. Recently, negative interference has also been found in immunoassays in the presence of rheumatoid factor. The chemiluminescent microparticle immunoassay is widely used to determine serum alpha-fetoprotein. However, it is not clear whether the presence of rheumatoid factor in the serum causes interference in the chemiluminescent microparticle immunoassay of alpha-fetoprotein. Methods Serum alpha-fetoprotein was determined using the ARCHITECT alpha-fetoprotein assay. The estimation of alpha-fetoprotein recovery was carried out in samples prepared by diluting high-concentration alpha-fetoprotein serum with rheumatoid factor-positive or rheumatoid factor-negative serum. Paramagnetic microparticles coated with hepatitis B surface antigen–anti-HBs complexes were used to remove rheumatoid factor from the serum. Results The average recovery of alpha-fetoprotein was 88.4% and 93.8% in the rheumatoid factor-positive and rheumatoid factor-negative serum samples, respectively. The recovery of alpha-fetoprotein was significantly lower in the rheumatoid factor-positive serum samples than in the rheumatoid factor-negative serum samples. In two of five rheumatoid factor-positive samples, a large difference was found (9.8%) between the average alpha-fetoprotein recoveries in the serially diluted and initial recoveries. Fourteen rheumatoid factor-positive serum samples were pretreated with hepatitis B surface antigen-anti-HBs complex-coated paramagnetic microparticles. The alpha-fetoprotein concentrations measured in the pretreated samples increased significantly. Conclusions It was concluded that the alpha-fetoprotein chemiluminescent microparticle immunoassay is susceptible to interference by rheumatoid factor, leading to significantly lower results. Eliminating the incidence of negative interference from rheumatoid factor should be an important goal for immunoassay providers. In the meantime, laboratorians must remain alert to the negative interference by rheumatoid factor, and in some cases, pretreat rheumatoid factor-positive samples with blocking or absorbing reagents.

scholarly journals European Proficiency Testing Program for Molecular Detection and Quantitation of Hepatitis B Virus DNA

2001 ◽  
Vol 39 (12) ◽  
pp. 4407-4412 ◽  
Author(s):  
E. Valentine-Thon ◽  
A. M. van Loon ◽  
J. Schirm ◽  
J. Reid ◽  
P. E. Klapper ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Proficiency Testing ◽  
Molecular Detection ◽  
Testing Program ◽  
Hepatitis B Virus Dna ◽  
B Virus
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