scholarly journals Immune adherence hemagglutination test applied to the study of herpes simplex and varicella-zoster virus infections

1978 ◽  
Vol 7 (2) ◽  
pp. 114-117
Author(s):  
A Gillani ◽  
L Spence
Keyword(s):  
Herpes Simplex ◽  
Varicella Zoster Virus ◽  
Antibody Titer ◽  
Comparative Studies ◽  
Complement Fixation ◽  
Virus Infections ◽  
Varicella Zoster ◽  
Immune Adherence ◽  
History Of ◽  
Low Levels

The immune adherence hemagglutination (IAHA) test has been used successfully to detect antibody to herpes simplex (HS) virus and varicella-zoster (V-Z) virus. Comparative studies between the complement-fixation (CF) test and the IAHA test revealed that, in most cases, the IAHA test was more sensitive than the CF test. Furthermore, diagnosis on the basis of a fourfold change in antibody titer was made more rapidly by the IAHA test. The IAHA test was found to be a very simple and practical technique requiring only a few hours for completion compared with the conventional CF test which required up to 24 h. In addition, both sera and cerebrospinal fluids could be tested in very low dilutions in the IAHA test, so that very low levels of antibody could be detected. Also, the IAHA test detected antibody to V-Z virus more frequently than did the CF test in adults with a history of varicella occurring 9 to 30 years prior to sampling. The level of cross-reaction between HS and V-Z viruses was examined by both the CF test and the IAHA test, and no major differences between the two techniques were found.

scholarly journals Antibody Assays for Varicella-Zoster Virus: Comparison of Enzyme Immunoassay with Neutralization, Immune Adherence Hemagglutination, and Complement Fixation

1978 ◽  
Vol 8 (5) ◽  
pp. 545-552
Author(s):  
Bagher Forghani ◽  
Nathalie J. Schmidt ◽  
Juanita Dennis
Keyword(s):  
Varicella Zoster Virus ◽  
Complement Fixation ◽  
False Negative ◽  
Neutralizing Antibody ◽  
Specific Method ◽  
Older Individuals ◽  
Virus Infections ◽  
Varicella Zoster ◽  
Immune Adherence ◽  
Antibody Titers

An enzyme immunossay (EIA) was adapted for detection of antibody to varicella-zoster virus, and its sensitivity and specificity were compared with those of neutralization, immune adherence hemagglutination (IAHA), and complement fixation tests. Test sera showed little nonspecific reactivity in the EIA system, and valid results could usually be obtained at serum dilutions as low as 1:8. Demonstration of the presence or absence of varicella-zoster viral antibody by EIA showed 94% correlation with results obtained in neutralization tests, but EIA titers were 2- to 16-fold higher than neutralizing antibody titers. Results by IAHA showed 87% correlation with those obtained by neutralization. No false positive IAHA results were seen, but a number of false negative IAHA results were seen at the 1:8 serum dilution, particularly in older individuals. With increasing age (>40 years), and presumably increased time from varicella infection, neutralizing antibody levels generally declined to 1:8 or 1:16, EIA levels fell to 1:128 or 1:256, and IAHA and complement fixation antibody titers were usually <1:8 or 1:8. EIA and IAHA were as reliable as the neutralization and complement fixation tests for serodiagnosis of varicella and zoster infections. All tests demonstrated heterotypic varicella-zoster antibody titer rises in selected patients with initial herpes simplex virus infections, but fewer heterotypic responses were seen by EIA than by the other methods. EIA offers a rapid, sensitive, and specific method for varicella-zoster antibody assay that is applicable to use in a clinical setting.

scholarly journals Prevalence of Anti-Varicella-Zoster Virus Antibodies in French Infants under 15 Months of Age

2009 ◽  
Vol 16 (4) ◽  
pp. 484-487 ◽  
Author(s):  
Didier Pinquier ◽  
Arnaud Gagneur ◽  
Laurent Balu ◽  
Olivier Brissaud ◽  
Christèle Gras Le Guen ◽  
...  
Keyword(s):  
Varicella Zoster Virus ◽  
Pediatric Emergency ◽  
Rapid Decline ◽  
Time Resolved ◽  
Varicella Zoster ◽  
Emergency Units ◽  
History Of ◽  
Low Levels ◽  
First Time ◽  
Kinetics Of

ABSTRACT Varicella is a widespread disease of childhood resulting from primary infection with varicella-zoster virus (VZV). The objective of this study was to determine the kinetics of the decline of maternal anti-VZV antibodies in French infants between birth and the age of 15 months in order to estimate the duration of passively acquired maternal anti-VZV immunoglobulin G (IgG). This prospective multicenter study was conducted between October 2005 and January 2007 in the pediatric wards and/or pediatric emergency units of seven French hospitals scattered throughout the country. The level of anti-VZV IgG antibodies in serum was measured by a time-resolved fluorescence immunoassay (TRFIA) (the threshold considered positive is 150 mIU/ml). A total of 345 infants were included. Seventy-seven percent of mothers reported a history of varicella. A rapid decline in the prevalence of anti-VZV antibodies was observed during the first few months of life, with the mean antibody titer decreasing from 536 mIU/ml at birth and through 1 month to below the 150-mIU/ml threshold at 3 to 4 months. The half-life of passively acquired maternal immunoglobulins was around 6 weeks. Based on a large number of subjects, this study clearly demonstrated, for the first time in France, high levels of passively acquired maternal antibodies during the neonatal period, and it allowed us to estimate the duration of passively acquired maternal anti-VZV IgG in French infants. After 4 to 5 months, infants had very low levels of maternal anti-VZV IgG, below the 150-mIU/ml cutoff of the VZV IgG TRFIA.

Sign in / Sign up

Export Citation Format

Share Document