SnRK1 Phosphorylation of AL2 Delays Cabbage Leaf Curl Virus Infection in Arabidopsis

Hamdan, R.S., I.D. Ismail and I. Akel. 2021. Effect of Tomato Yellow Leaf Curl Virus Infection on Some Growth Indicators of Pepper Hybrids Grown Under Plastic House Conditions in Lattakia Governorate, Syria. Arab Journal of Plant Protection, 39(4): 309-316. https://doi.org/10.22268/AJPP-39.4.309316 This study aimed to evaluate the effect of Tomato yellow leaf curl virus (TYLCV) infection on some growth parameters of several pepper hybrids grown under protected cultivation along the Syrian coast. Four pepper hybrids (Capsicum annum) were tested, two hot (Lahab, Sirad) and two sweet (Nevada, Dallas) peppers. The research was carried out during the 2020-2021 growing season in a plastic house at the Agriculture Scientific Research Center in Lattakia, using a randomized complete block design, with 8 treatments, three replicates and 5 plants per replicate. The results obtained showed that the sweet pepper hybrids (Nevada, Dallas) were more sensitive to infection with Tomato yellow leaf curl virus than the hot pepper hybrids (Lahab, Sirad), without significant differences among them. The pepper hybrid Nevada did not show any apparent symptoms, and the effect of the virus on some growth parameters (number flowers, flower setting, and the leaf surface area) was evident in comparison with other hybrids, but the differences were not significant. The Dallas, Sirad and Lahab pepper hybrids showed visible symptoms in response to infection with infection rate of 93.33%, 80% and 80%, respectively. The effect of virus infection on the growth parameters of these hybrids was variable. The reduction in the flowers number of Nevada, Dallas, Cirad and Lahab after 45 days of infection was 33%, 31.10%, 10.23% and 5.07%, respectively, and also with a reduction in the number of flowers setting of 77.39%, 20.87%, 23.33% and 66.68%, respectively. The same was true for the leaf surface area, where reduction rate for the Nevada, Dallas, Sirad and Lahab hybrids 30 days after infection was 48.17%, 53.06%, 16.45% and 36.6%, respectively. Keywords: Tomato yellow leaf curl virus, pepper hybrids, growth parameter, host resistance, protected agriculture.

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Silencing of tomato CTR1 provides enhanced tolerance against Tomato leaf curl virus infection

Plant Signaling & Behavior ◽

10.1080/15592324.2019.1565595 ◽

2019 ◽

Vol 14 (3) ◽

pp. e1565595 ◽

Cited By ~ 2

Author(s):

Ravindra K. Chandan ◽

Achuit K. Singh ◽

Sunita Patel ◽

Durga Madhab Swain ◽

Narendra Tuteja ◽

...

Keyword(s):

Virus Infection ◽

Tomato Leaf ◽

Tomato Leaf Curl Virus ◽

Tomato Leaf Curl ◽

Leaf Curl Virus ◽

Leaf Curl

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An efficient virus-induced gene silencing (VIGS) system for functional genomics in Brassicas using a cabbage leaf curl virus (CaLCuV)-based vector

Planta ◽

10.1007/s00425-020-03454-7 ◽

2020 ◽

Vol 252 (3) ◽

Author(s):

Zhiliang Xiao ◽

Miaomiao Xing ◽

Xing Liu ◽

Zhiyuan Fang ◽

Limei Yang ◽

...

Keyword(s):

Gene Silencing ◽

Functional Genomics ◽

Virus Induced Gene Silencing ◽

Cabbage Leaf Curl Virus ◽

Leaf Curl Virus ◽

Leaf Curl

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Maintenance of an Old World Betasatellite by a New World Helper Begomovirus and Possible Rapid Adaptation of the Betasatellite

Journal of Virology ◽

10.1128/jvi.00795-09 ◽

2009 ◽

Vol 83 (18) ◽

pp. 9347-9355 ◽

Cited By ~ 64

Author(s):

Muhammad Shah Nawaz-ul-Rehman ◽

Shahid Mansoor ◽

Rob W. Briddon ◽

Claude M. Fauquet

Keyword(s):

New World ◽

Cotton Leaf ◽

Old World ◽

Tropical Regions ◽

Cabbage Leaf Curl Virus ◽

Chili Leaf Curl Betasatellite ◽

Serious Disease ◽

Leaf Curl Virus ◽

First Time ◽

Leaf Curl

ABSTRACT Begomoviruses (family Geminiviridae) cause major losses to crops throughout the tropical regions of the world. Begomoviruses originating from the New World (NW) and the Old World (OW) are genetically distinct. Whereas the majority of OW begomoviruses have monopartite genomes and whereas most of these associate with a class of symptom-modulating satellites (known as betasatellites), the genomes of NW begomoviruses are exclusively bipartite and do not associate with satellites. Here, we show for the first time that a betasatellite (cotton leaf curl Multan betasatellite [CLCuMuB]) associated with a serious disease of cotton across southern Asia is capable of interacting with a NW begomovirus. In the presence of CLCuMuB, the symptoms of the NW cabbage leaf curl virus (CbLCuV) are enhanced in Nicotiana benthamiana. However, CbLCuV was unable to interact with a second betasatellite, chili leaf curl betasatellite. Although CbLCuV can transreplicate CLCuMuB, satellite accumulation levels in plants were low. However, progeny CLCuMuB isolated after just one round of infection with CbLCuV contained numerous mutations. Reinoculation of one such progeny CLCuMuB with CbLCuV to N. benthamiana yielded infections with significantly higher satellite DNA levels. This suggests that betasatellites can rapidly adapt for efficient transreplication by a new helper begomovirus, including begomoviruses originating from the NW. Although the precise mechanism of transreplication of betasatellites by begomoviruses remains unknown, an analysis of betasatellite mutants suggests that the sequence(s) required for maintenance of CLCuMuB by one of its cognate begomoviruses (cotton leaf curl Rajasthan virus) differs from the sequences required for maintenance by CbLCuV. The significance of these findings and, particularly, the threat that betasatellites pose to agriculture in the NW, are discussed.

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