scholarly journals Complete Genome Sequence of the Broad Host Range Single-Stranded RNA Phage PRR1 Places It in the Levivirus Genus with Characteristics Shared with Alloleviviruses

2006 ◽  
Vol 80 (18) ◽  
pp. 9326-9330 ◽  
Author(s):  
Tanja M. Ruokoranta ◽  
A. Marika Grahn ◽  
Janne J. Ravantti ◽  
Minna M. Poranen ◽  
Dennis H. Bamford

ABSTRACT Single-stranded RNA (ssRNA) bacteriophages of the family Leviviridae infect gram-negative bacteria. They are restricted to a single host genus. Phage PRR1 is an exception, having a broad host range due to the promiscuity of the receptor encoded by the IncP plasmid. Here we report the complete genome sequence of PRR1. Three proteins homologous with those of other ssRNA phages, i.e., maturation, coat, and replicase proteins, were identified. A fourth protein has a lysis function. Comparison of PRR1 with other members of the Leviviridae family places PRR1 in the genus Levivirus with some characteristics more similar to those of members of the genus Allolevivirus.

2012 ◽  
Vol 194 (16) ◽  
pp. 4483-4483 ◽  
Author(s):  
J. Schuldes ◽  
M. Rodriguez Orbegoso ◽  
C. Schmeisser ◽  
H. B. Krishnan ◽  
R. Daniel ◽  
...  

2003 ◽  
Vol 185 (17) ◽  
pp. 5220-5233 ◽  
Author(s):  
Eric S. Miller ◽  
John F. Heidelberg ◽  
Jonathan A. Eisen ◽  
William C. Nelson ◽  
A. Scott Durkin ◽  
...  

ABSTRACT The complete genome sequence of the T4-like, broad-host-range vibriophage KVP40 has been determined. The genome sequence is 244,835 bp, with an overall G+C content of 42.6%. It encodes 386 putative protein-encoding open reading frames (CDSs), 30 tRNAs, 33 T4-like late promoters, and 57 potential rho-independent terminators. Overall, 92.1% of the KVP40 genome is coding, with an average CDS size of 587 bp. While 65% of the CDSs were unique to KVP40 and had no known function, the genome sequence and organization show specific regions of extensive conservation with phage T4. At least 99 KVP40 CDSs have homologs in the T4 genome (Blast alignments of 45 to 68% amino acid similarity). The shared CDSs represent 36% of all T4 CDSs but only 26% of those from KVP40. There is extensive representation of the DNA replication, recombination, and repair enzymes as well as the viral capsid and tail structural genes. KVP40 lacks several T4 enzymes involved in host DNA degradation, appears not to synthesize the modified cytosine (hydroxymethyl glucose) present in T-even phages, and lacks group I introns. KVP40 likely utilizes the T4-type sigma-55 late transcription apparatus, but features of early- or middle-mode transcription were not identified. There are 26 CDSs that have no viral homolog, and many did not necessarily originate from Vibrio spp., suggesting an even broader host range for KVP40. From these latter CDSs, an NAD salvage pathway was inferred that appears to be unique among bacteriophages. Features of the KVP40 genome that distinguish it from T4 are presented, as well as those, such as the replication and virion gene clusters, that are substantially conserved.


PLoS Genetics ◽  
2008 ◽  
Vol 4 (7) ◽  
pp. e1000141 ◽  
Author(s):  
Derrick E. Fouts ◽  
Heather L. Tyler ◽  
Robert T. DeBoy ◽  
Sean Daugherty ◽  
Qinghu Ren ◽  
...  

2014 ◽  
Vol 159 (11) ◽  
pp. 3153-3155 ◽  
Author(s):  
Robert Czajkowski ◽  
Zofia Ozymko ◽  
Szymon Zwirowski ◽  
Ewa Lojkowska

2012 ◽  
Vol 157 (1-2) ◽  
pp. 164-171 ◽  
Author(s):  
Ji Hyung Kim ◽  
Jee Soo Son ◽  
Yun Jaie Choi ◽  
Casiano H. Choresca ◽  
Sang Phil Shin ◽  
...  

2018 ◽  
Vol 6 (25) ◽  
Author(s):  
Mingrui Duan ◽  
Samuel S. Hunter ◽  
Scott A. Minnich ◽  
Matthew W. Fagnan ◽  
Daniel D. New ◽  
...  

ABSTRACT The Shiga toxin-encoding phage SH2026Stx1 was isolated from Escherichia coli O157:H7 strain 2026. SH2026Stx1 and its detoxified derivative can infect a broad range of E. coli strains, including commensal, enteropathogenic, and enteroaggregative strains. We report here the complete genome sequence of phage SH2026Stx1 and its important features.


2014 ◽  
Vol 9 (3) ◽  
pp. 763-774 ◽  
Author(s):  
Lionel Moulin ◽  
Agnieszka Klonowska ◽  
Bournaud Caroline ◽  
Kristina Booth ◽  
Jan A.C. Vriezen ◽  
...  

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Claudia Jäckel ◽  
Eckhard Strauch ◽  
Jens Andre Hammerl

Vibrio choleraeis the cause of large cholera outbreaks, especially in endemic regions with high poverty and inadequate sanitation. Here, we announce the complete genome sequence of the virulence-associated broad host rangeV. choleraephage VcP032, including a brief summary of its genotypic and phenotypic features.


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