scholarly journals The Nucleotides on the Stem-Loop RNA Structure in the Junction Region of the Hepatitis E Virus Genome Are Critical for Virus Replication

2010 ◽  
Vol 84 (24) ◽  
pp. 13040-13044 ◽  
Author(s):  
Dianjun Cao ◽  
Yao-Wei Huang ◽  
Xiang-Jin Meng
Keyword(s):  
Virus Replication ◽  
Intergenic Region ◽  
Rna Structure ◽  
Hepatitis E Virus ◽  
Reporter Genes ◽  
Hepatitis E ◽  
Virus Genome ◽  
Junction Region ◽  
Stem Loop ◽  
Huh7 Cells

ABSTRACT The roles of conserved nucleotides on the stem-loop (SL) structure in the intergenic region of the hepatitis E virus (HEV) genome in virus replication were determined by using Huh7 cells transfected with HEV SL mutant replicons containing reporter genes. One or two nucleotide mutations of the AGA motif on the loop significantly reduced HEV replication, and three or more nucleotide mutations on the loop abolished HEV replication. Mutations on the stem and of the subgenome start sequence also significantly inhibited HEV replication. The results indicated that both the sequence and the SL structure in the junction region play important roles in HEV replication.

scholarly journals NMR study on the interaction of the conserved CREX ‘stem–loop’ in the Hepatitis E virus genome with a naphthyridine-based ligand

2015 ◽  
Vol 13 (37) ◽  
pp. 9665-9672 ◽  
Author(s):  
N. Dyubankova ◽  
M. Froeyen ◽  
M. Abramov ◽  
H. P. Mattelaer ◽  
P. Herdewijn ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Genome ◽  
Stem Loop ◽  
Dna And Rna ◽  
Nmr Study ◽  
Rna Duplexes

A 2-amino-1,8-naphthyridine derivative that is described to bind single guanine bulges in RNA–DNA and RNA–RNA duplexes was synthesized and its interaction with the single G bulge in the conserved CREX of the Hepatitis E Virus (HEV) genome was explored by NMR and molecular modeling.

scholarly journals Activation of viral transcription by stepwise largescale folding of an RNA virus genome

2020 ◽  
Vol 48 (16) ◽  
pp. 9285-9300
Author(s):  
Tamari Chkuaseli ◽  
K Andrew White
Keyword(s):  
Rna Structure ◽  
Rna Viruses ◽  
Rna Virus ◽  
Initial Step ◽  
Binding Pocket ◽  
Regulatory Elements ◽  
Virus Genome ◽  
Genomic Rna ◽  
Stem Loop ◽  
Rna Complex

Abstract The genomes of RNA viruses contain regulatory elements of varying complexity. Many plus-strand RNA viruses employ largescale intra-genomic RNA-RNA interactions as a means to control viral processes. Here, we describe an elaborate RNA structure formed by multiple distant regions in a tombusvirus genome that activates transcription of a viral subgenomic mRNA. The initial step in assembly of this intramolecular RNA complex involves the folding of a large viral RNA domain, which generates a discontinuous binding pocket. Next, a distally-located protracted stem-loop RNA structure docks, via base-pairing, into the binding site and acts as a linchpin that stabilizes the RNA complex and activates transcription. A multi-step RNA folding pathway is proposed in which rate-limiting steps contribute to a delay in transcription of the capsid protein-encoding viral subgenomic mRNA. This study provides an exceptional example of the complexity of genome-scale viral regulation and offers new insights into the assembly schemes utilized by large intra-genomic RNA structures.

scholarly journals Pan-Genotype Hepatitis E Virus Replication in Stem Cell–Derived Hepatocellular Systems

2018 ◽  
Vol 154 (3) ◽  
pp. 663-674.e7 ◽  
Author(s):  
Xianfang Wu ◽  
Viet Loan Dao Thi ◽  
Peng Liu ◽  
Constantin N. Takacs ◽  
Kuanhui Xiang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Virus Replication ◽  
Hepatitis E Virus ◽  
Hepatitis E

scholarly journals Inhibition of hepatitis E virus replication by peptide-conjugated morpholino oligomers

2015 ◽  
Vol 120 ◽  
pp. 134-139 ◽  
Author(s):  
Yuchen Nan ◽  
Zexu Ma ◽  
Harilakshmi Kannan ◽  
David A. Stein ◽  
Patrick I. Iversen ◽  
...  
Keyword(s):  
Virus Replication ◽  
Hepatitis E Virus ◽  
Hepatitis E

RIG-I Inhibits Hepatitis E Virus Replication by Simultaneously Activating JAK-STAT and NFKB Pathways

2016 ◽  
Vol 64 (2) ◽  
pp. S518
Author(s):  
L. Xu ◽  
W. Wang ◽  
X. Zhou ◽  
Y. Wang ◽  
Y. Yin ◽  
...  
Keyword(s):  
Virus Replication ◽  
Hepatitis E Virus ◽  
Hepatitis E

Hepatitis E virus genome in stools of hepatitis patients during large epidemic in north India

The Lancet ◽  
1991 ◽  
Vol 338 (8770) ◽  
pp. 783-784 ◽  
Author(s):  
R. Ray ◽  
G.P. Talwar ◽  
R. Aggarwal ◽  
P.N. Salunke ◽  
S.R. Naik ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Genome ◽  
North India

scholarly journals Novel hepatitis E like virus found in Swedish moose

2014 ◽  
Vol 95 (3) ◽  
pp. 557-570 ◽  
Author(s):  
Jay Lin ◽  
Heléne Norder ◽  
Henrik Uhlhorn ◽  
Sándor Belák ◽  
Frederik Widén
Keyword(s):  
Hepatitis E Virus ◽  
Sequence Similarity ◽  
Mammalian Species ◽  
Hepatitis E ◽  
Human Infection ◽  
Stem Loop ◽  
Novel Virus ◽  
Human Infections ◽  
Transmission Pathways

A novel virus was detected in a sample collected from a Swedish moose (Alces alces). The virus was suggested as a member of the Hepeviridae family, although it was found to be highly divergent from the known four genotypes (gt1–4) of hepatitis E virus (HEV). Moose are regularly hunted for consumption in the whole of Scandinavia. Thus, the finding of this virus may be important from several aspects: (a) as a new diverged HEV in a new animal species, and (b) potential unexplored HEV transmission pathways for human infections. Considering these aspects, we have started the molecular characterization of this virus. A 5.1 kb amplicon was sequenced, and corresponded to the partial ORF1, followed by complete ORF2, ORF3 and poly(A) sequence. In comparison with existing HEVs, the moose HEV genome showed a general nucleotide sequence similarity of 37–63 % and an extensively divergent putative ORF3 sequence. The junction region between the ORFs was also highly divergent; however, two putative secondary stem–loop structures were retained when compared to gt1–4, but with altered structural appearance. In the phylogenetic analysis, the moose HEV deviated and formed its own branch between the gt1–4 and other divergent animal HEVs. The characterization of this highly divergent genome provides important information regarding the diversity of HEV infecting various mammalian species. However, further studies are needed to investigate its prevalence in the moose populations and possibly in other host species, including the risk for human infection.

scholarly journals Hepatitis E virus replication involves alternating negative- and positive-sense RNA synthesis

2010 ◽  
Vol 92 (3) ◽  
pp. 572-581 ◽  
Author(s):  
S. P. K. Varma ◽  
A. Kumar ◽  
N. Kapur ◽  
H. Durgapal ◽  
S. K. Acharya ◽  
...  
Keyword(s):  
Virus Replication ◽  
Hepatitis E Virus ◽  
Rna Synthesis ◽  
Hepatitis E ◽  
Positive Sense
Sign in / Sign up

Export Citation Format

Share Document