scholarly journals Selective Packaging of Host tRNA's by Murine Leukemia Virus Particles Does Not Require Genomic RNA

1979 ◽  
Vol 29 (1) ◽  
pp. 328-335 ◽  
Author(s):  
Judith G. Levin ◽  
J. G. Seidman
Keyword(s):  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Genomic Rna ◽  
Virus Particles ◽  
Murine Leukemia

Recent Studies on a Murine Leukemia Virus Grown in Tissue Culture

1967 ◽  
Vol 25 ◽  
pp. 106-107
Author(s):  
L. Z. de Tkaczevski ◽  
E. de Harven ◽  
C. Friend
Keyword(s):  
Tissue Culture ◽  
Solid Tumors ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Supernatant Fluid ◽  
Virus Particles ◽  
Friend Leukemia ◽  
Type C ◽  
Leukemia Viruses ◽  
Murine Leukemia

Despite extensive studies, the correlation between the morphology and pathogenicity of murine leukemia viruses (MLV) has not yet been clarified. The virus particles found in the plasma of leukemic mice belong to 2 distinct groups, 1 or 2% of them being enveloped A particles and the vast majority being of type C. It is generally believed that these 2 types of particles represent different phases in the development of the same virus. Particles of type A have been thought to be an earlier form of type C particles. One of the tissue culture lines established from Friend leukemia solid tumors has provided the material for the present study. The supernatant fluid of the line designated C-1A contains an almost pure population of A particles as illustrated in Figure 1. The ratio is, therefore, the reverse of what is unvariably observed in the plasma of leukemic mice where C particles predominate.

scholarly journals mRNA Molecules Containing Murine Leukemia Virus Packaging Signals Are Encapsidated as Dimers

2004 ◽  
Vol 78 (20) ◽  
pp. 10927-10938 ◽  
Author(s):  
Catherine S. Hibbert ◽  
Jane Mirro ◽  
Alan Rein
Keyword(s):  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Moloney Murine Leukemia Virus ◽  
Genomic Rna ◽  
Packaging Signal ◽  
Electron Microscopic ◽  
Genomic Rnas ◽  
Microscopic Studies ◽  
The Stability ◽  
Murine Leukemia

ABSTRACT Prior work by others has shown that insertion of ψ (i.e., leader) sequences from the Moloney murine leukemia virus (MLV) genome into the 3′ untranslated region of a nonviral mRNA leads to the specific encapsidation of this RNA in MLV particles. We now report that these RNAs are, like genomic RNAs, encapsidated as dimers. These dimers have the same thermostability as MLV genomic RNA dimers; like them, these dimers are more stable if isolated from mature virions than from immature virions. We characterized encapsidated mRNAs containing deletions or truncations of MLV ψ or with ψ sequences from MLV-related acute transforming viruses. The results indicate that the dimeric linkage in genomic RNA can be completely attributed to the ψ region of the genome. While this conclusion agrees with earlier electron microscopic studies on mature MLV dimers, it is the first evidence as to the site of the linkage in immature dimers for any retrovirus. Since the Ψ+ mRNA is not encapsidated as well as genomic RNA, it is only present in a minority of virions. The fact that it is nevertheless dimeric argues strongly that two of these molecules are packaged into particles together. We also found that the kissing loop is unnecessary for this coencapsidation or for the stability of mature dimers but makes a major contribution to the stability of immature dimers. Our results are consistent with the hypothesis that the packaging signal involves a dimeric structure in which the RNAs are joined by intermolecular interactions between GACG loops.

scholarly journals Determinants of Moloney Murine Leukemia Virus Gag-Pol and Genomic RNA Proportions

2014 ◽  
Vol 88 (13) ◽  
pp. 7267-7275 ◽  
Author(s):  
S. F. Johnson ◽  
J. T. Collins ◽  
V. M. D'Souza ◽  
A. Telesnitsky
Keyword(s):  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Moloney Murine Leukemia Virus ◽  
Genomic Rna ◽  
Murine Leukemia

scholarly journals Incorporation of Fowl Plague Virus Hemagglutinin into Murine Leukemia Virus Particles and Analysis of the Infectivity of the Pseudotyped Retroviruses

1998 ◽  
Vol 72 (6) ◽  
pp. 5313-5317 ◽  
Author(s):  
Theodora Hatziioannou ◽  
Sandrine Valsesia-Wittmann ◽  
Stephen J. Russell ◽  
François-Loïc Cosset
Keyword(s):  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Retroviral Vector ◽  
Moloney Murine Leukemia Virus ◽  
Wild Type ◽  
Cell Surface Molecule ◽  
Virus Attachment ◽  
Virus Particles ◽  
Fusion Ability ◽  
Murine Leukemia

ABSTRACT We describe retrovirus particles carrying the fowl plague virus (FPV) hemagglutinin (HA). When expressed in cells providing Moloney murine leukemia virus (MoMLV) Gag and Pol proteins and alacZ retroviral vector, FPV HA was found to be efficiently expressed, correctly processed, and stably incorporated into retroviral particles. HA-bearing retroviruses were infectious with a wide host range and were only 10-fold less infectious than retroviruses carrying wild-type MLV retroviral envelopes. We also coexpressed HA proteins in retroviral particles with chimeric MoMLV-derived envelope glycoproteins that efficiently retarget virus attachment but are only weakly fusogenic. Our results suggest that HA can in some cases enhance the fusion ability of these retroviral particles, depending on the cell surface molecule that is used as a receptor.

Immunologic Localization of Murine Leukemia Virus Antigens in Thin Sections

1981 ◽  
Vol 39 ◽  
pp. 402-403
Author(s):  
Ray A. Weigand ◽  
Carol I. Paquette ◽  
Clifford Longley ◽  
Philip Furmanski
Keyword(s):  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Intracellular Localization ◽  
Protein A ◽  
Thin Sections ◽  
Virus Particles ◽  
Tumor Virus ◽  
Labelling Procedure ◽  
Nih 3T3 ◽  
Murine Leukemia

In order to examine the temporal sequence of intracellular events which culminates in the release of infectious virus particles from the cell surface, we have developed immunoferritin techniques to localize viral antigens in thin sections. We previously described the intracellular localization of murine mammary tumor virus p28 protein in thin sections using an unlabelled antibody procedure with ferritin-antiferritin. We now describe the labelling of murine leukemia virus (MuLV) in thin sections using anti-MuLV and Protein A-ferritin.Cultures of F-MuLV in NIH/3T3 cells were grown to 90% confluence, fixed, dehydrated, and embedded in Epon as before. Thin sections were picked up on nickel grids, incubated in 20% H2O2 for 20 min, rinsed in PBS, and subjected to the labelling procedure. The labelling protocol included: preincubation in 5% BSA in PBS, rinse in 1% BSA in PBS, 3 hour incubation in rabbit anti-MuLV, rinse in 1% BSA in PBS, incubation in apoferritin, incubation in Protein A-ferritin (Zymed Labs., Burlingame, CA), rinses in 1% BSA in PBS and PBS, and incubation in 2% glutaraldehyde in PBS.

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