Determination of viral proteins present in the human immunodeficiency virus type 1 preintegration complex.

ABSTRACT We developed a recombinant virus technique to determine the coreceptor usage of human immunodeficiency virus type 1 (HIV-1) from plasma samples, the source expected to represent the most actively replicating virus population in infected subjects. This method is not subject to selective bias associated with virus isolation in culture, a step required for conventional tropism determination procedures. The addition of a simple subcloning step allowed semiquantitative evaluation of virus populations with a different coreceptor (CCR5 or CXCR4) usage specificity present in each plasma sample. This procedure detected mixtures of CCR5- and CXCR4-exclusive virus populations as well as dualtropic viral variants, in variable proportions. Sequence analysis of dualtropic clones indicated that changes in the V3 loop are necessary for the use of CXCR4 as a coreceptor, but the overall context of the V1-V3 region is important to preserve the capacity to use CCR5. This convenient technique can greatly assist the study of virus evolution and compartmentalization in infected individuals.

Download Full-text

Fluorescent Dye Terminator Sequencing Methods for Quantitative Determination of Replication Fitness of Human Immunodeficiency Virus Type 1 Containing the Codon 74 and 184 Mutations in Reverse Transcriptase

Journal of Clinical Microbiology ◽

10.1128/jcm.41.7.3306-3311.2003 ◽

2003 ◽

Vol 41 (7) ◽

pp. 3306-3311 ◽

Cited By ~ 17

Author(s):

V. Nurpeisov ◽

S. J. Hurwitz ◽

P. L. Sharma

Keyword(s):

Human Immunodeficiency Virus ◽

Reverse Transcriptase ◽

Quantitative Determination ◽

Human Immunodeficiency Virus Type ◽

Virus Type ◽

Fluorescent Dye ◽

Immunodeficiency Virus ◽

Replication Fitness

Download Full-text

Combination of whole blood culture and a rapid and sensitive cell assay for the determination of the cytopathogenicity of human immunodeficiency virus type-1 isolates

Journal of Virological Methods ◽

10.1016/s0166-0934(97)00210-3 ◽

1998 ◽

Vol 71 (1) ◽

pp. 123-131 ◽

Cited By ~ 1

Author(s):

Samira Benyoucef ◽

Didier Hober ◽

Georges Lion ◽

Virginie Vilain ◽

Laurence Bocket ◽

...

Keyword(s):

Human Immunodeficiency Virus ◽

Blood Culture ◽

Human Immunodeficiency Virus Type ◽

Virus Type ◽

Whole Blood ◽

Sensitive Cell ◽

Cell Assay ◽

Immunodeficiency Virus

Download Full-text

Importance of method in the determination of syncytium-inducing phenotype of human immunodeficiency virus type 1 clinical isolates

Journal of Virological Methods ◽

10.1016/s0166-0934(96)02152-0 ◽

1997 ◽

Vol 64 (2) ◽

pp. 137-145 ◽

Cited By ~ 3

Author(s):

Corinne Liesnard ◽

Marie-Luce Delforge ◽

Michel Tchetcheroff ◽

Viviane De Maertelaer ◽

Claire-Michele Farber ◽

...

Keyword(s):

Human Immunodeficiency Virus ◽

Human Immunodeficiency Virus Type ◽

Virus Type ◽

Clinical Isolates ◽

Immunodeficiency Virus

Download Full-text

Identification of RFPL3 Protein as a Novel E3 Ubiquitin Ligase Modulating the Integration Activity of Human Immunodeficiency Virus, Type 1 Preintegration Complex Using a Microtiter Plate-based Assay

Journal of Biological Chemistry ◽

10.1074/jbc.m114.561662 ◽

2014 ◽

Vol 289 (38) ◽

pp. 26368-26382 ◽

Cited By ~ 7

Author(s):

Beng Hui Tan ◽

Yasutsugu Suzuki ◽

Hirotaka Takahashi ◽

Pamela Ho Rui Ying ◽

Chikako Takahashi ◽

...

Keyword(s):

Human Immunodeficiency Virus ◽

Human Immunodeficiency Virus Type ◽

Virus Type ◽

Ubiquitin Ligase ◽

E3 Ubiquitin Ligase ◽

Microtiter Plate ◽

Preintegration Complex ◽

Immunodeficiency Virus

Download Full-text

Determination of antibodies to human immunodeficiency virus type 1&2&O and P24 – antigen in pregnant women in port harcourt Nigeria

Journal of Immunoassay and Immunochemistry ◽

10.1080/15321819.2019.1708387 ◽

2019 ◽

Vol 41 (2) ◽

pp. 208-218

Author(s):

Iheanyi Omezuruike Okonko ◽

Eberechukwu Maryann Okoli

Keyword(s):

Human Immunodeficiency Virus ◽

Pregnant Women ◽

Human Immunodeficiency Virus Type ◽

Virus Type ◽

Port Harcourt ◽

Immunodeficiency Virus ◽

P24 Antigen

Download Full-text

Effect of retinoic acid on HL-60 cells infected with human immunodeficiency virus type 1

Blood ◽

10.1182/blood.v84.8.2480.2480 ◽

1994 ◽

Vol 84 (8) ◽

pp. 2480-2488 ◽

Cited By ~ 10

Author(s):

M Semmel ◽

A Macho ◽

D Coulaud ◽

A Alileche ◽

S Plaisance ◽

...

Keyword(s):

Human Immunodeficiency Virus ◽

Retinoic Acid ◽

Human Immunodeficiency Virus Type ◽

Virus Type ◽

Viral Proteins ◽

Mrna Synthesis ◽

Immunodeficiency Virus ◽

Infected Cells ◽

Hiv 1

Abstract HL-60 cells infected with human immunodeficiency virus type 1 (HIV 1) can be induced to differentiate along the granulocyte pathway by retinoic acid. In these cells, HIV mRNA synthesis is stimulated, but synthesis of viral proteins and virus replication are blocked and HIV- infected cells die after becoming apoptotic and/or vacuolized.

Download Full-text

Determination of Essential Amino Acids Involved in the CD4-Independent Tropism of the X4 Human Immunodeficiency Virus Type 1 m7NDK Isolate: Role of Potential N Glycosylations in the C2 and V3 Regions of gp120

Journal of Virology ◽

10.1128/jvi.75.11.5425-5428.2001 ◽

2001 ◽

Vol 75 (11) ◽

pp. 5425-5428 ◽

Cited By ~ 19

Author(s):

Julie Dumonceaux ◽

Caroline Goujon ◽

Veronique Joliot ◽

Pascale Briand ◽

Uriel Hazan

Keyword(s):

Amino Acids ◽

Human Immunodeficiency Virus ◽

Human Immunodeficiency Virus Type ◽

Virus Type ◽

Essential Amino Acids ◽

Site Directed Mutagenesis ◽

Immunodeficiency Virus

ABSTRACT Seven mutations in the C2, V3, and C3 regions of gp120 are implicated in the tropism of the first CD4-independent human immunodeficiency virus type 1 isolate, m7NDK. Site-directed mutagenesis revealed that three amino acids are essential to maintain this tropism, one in the C2 region and two in the V3 loop. Two mutations implied N glycosylation modifications.

Download Full-text