Expression of functional Bunyamwera virus L protein by recombinant vaccinia viruses.

1991 ◽  
Vol 65 (8) ◽  
pp. 4182-4189 ◽  
Author(s):  
H Jin ◽  
R M Elliott
Keyword(s):  
Recombinant Vaccinia ◽  
L Protein ◽  
Vaccinia Viruses ◽  
Bunyamwera Virus

Protection of mice from respiratory Sendai virus infections by recombinant vaccinia viruses.

1997 ◽  
Vol 71 (1) ◽  
pp. 832-838 ◽  
Author(s):  
S I Takao ◽  
K Kiyotani ◽  
T Sakaguchi ◽  
Y Fujii ◽  
M Seno ◽  
...  
Keyword(s):  
Sendai Virus ◽  
Virus Infections ◽  
Recombinant Vaccinia ◽  
Vaccinia Viruses

scholarly journals Anchoring a secreted plasmodium antigen on the surface of recombinant vaccinia virus-infected cells increases its immunogenicity.

1986 ◽  
Vol 6 (9) ◽  
pp. 3191-3199 ◽  
Author(s):  
C J Langford ◽  
S J Edwards ◽  
G L Smith ◽  
G F Mitchell ◽  
B Moss ◽  
...  
Keyword(s):  
Transmembrane Domain ◽  
Poor Response ◽  
Subcellular Location ◽  
Epidemiological Data ◽  
Recombinant Vaccinia Virus ◽  
Live Virus ◽  
Recombinant Vaccinia ◽  
Wide Range ◽  
Vaccinia Viruses ◽  
Infected Cells

We show that the subcellular location of foreign antigens expressed in recombinant vaccinia viruses influences their effectiveness as immunogens. Live recombinant viruses induced very poor antibody responses to a secreted repetitive plasmodial antigen (the S-antigen) in rabbits and mice. The poor response accords with epidemiological data suggesting that S-antigens are poorly immunogenic. Appending the transmembrane domain of a membrane immunoglobulin (immunoglobulin G1) to its carboxy terminus produced a hybrid S-antigen that was no longer secreted but was located on the surface of virus-infected cells. This recombinant virus elicited high antibody titers to the S-antigen. This approach will facilitate the use of live virus delivery systems to immunize against a wide range of foreign nonsurface antigens.

scholarly journals Recombinant Vaccinia Viruses as New Live Vaccines

1984 ◽  
Vol 2 (1) ◽  
pp. 383-407 ◽  
Author(s):  
Geoffrey L. Smith ◽  
Michael Mackett ◽  
Bernard Moss
Keyword(s):  
Recombinant Vaccinia ◽  
Live Vaccines ◽  
Vaccinia Viruses

scholarly journals Construction and characterization of recombinant vaccinia viruses co-expressing a respiratory syncytial virus protein and a cytokine

2001 ◽  
Vol 82 (9) ◽  
pp. 2107-2116 ◽  
Author(s):  
Teresa R. Johnson ◽  
Julie E. Fischer ◽  
Barney S. Graham
Keyword(s):  
Respiratory Syncytial Virus ◽  
Immune Responses ◽  
Vaccinia Virus ◽  
Antigen Expression ◽  
Virus Protein ◽  
Recombinant Vaccinia ◽  
Vaccinia Viruses ◽  
Ifn Γ ◽  
Syncytial Virus ◽  
The Impact

Recombinant vaccinia viruses are well-characterized tools that can be used to define novel approaches to vaccine formulation and delivery. While vector co-expression of immune mediators has enormous potential for optimizing the composition of vaccine-induced immune responses, the impact on antigen expression and vector antigenicity must also be considered. Co-expression of IL-4 increased vaccinia virus vector titres, while IFN-γ co-expression reduced vaccinia virus replication in BALB/c mice and in C57BL/6 mice infected with some recombinant viruses. Protection against respiratory syncytial virus (RSV) challenge was similar in mice immunized with vaccinia virus expressing RSV G glycoprotein and IFN-γ, even though the replication efficiency of the vector was diminished. These data demonstrate the ability of vector-expressed cytokine to influence the virulence of the vector and to direct the development of selected immune responses. This suggests that the co-expression of cytokines and other immunomodulators has the potential to improve the safety of vaccine vectors while improving the immunogenicity of vaccine antigens.

Two independent domains of Factor VIII co-expressed using recombinant vaccinia viruses have procoagulant activity

1987 ◽  
Vol 145 (1) ◽  
pp. 234-240 ◽  
Author(s):  
Andréa Pavirani ◽  
Pierre Meulien ◽  
Huguette Harrer ◽  
Karin Dott ◽  
Fabienne Mischler ◽  
...  
Keyword(s):  
Factor Viii ◽  
Procoagulant Activity ◽  
Recombinant Vaccinia ◽  
Vaccinia Viruses
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