Detection of Viral Proteins after Infection of Cultured Hepatocytes with Rabbit Hemorrhagic Disease Virus
1998 ◽
Vol 72
(5)
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pp. 4492-4497
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Keyword(s):
ABSTRACT The calicivirus rabbit hemorrhagic disease virus (RHDV), which replicates predominantly in the livers of infected rabbits, cannot be propagated in tissue culture. To enable the performance of in vitro studies, rabbit hepatocytes were isolated by liver perfusion and gradient centrifugation. After inoculation with purified RHDV, more than 50% of the cells proved to be infected. Protein analyses led to the detection of 13 RHDV-specific polypeptides within the infected cells. These proteins were assigned to defined regions of the viral genome, resulting in a refined model of RHDV genome organization.
1998 ◽
Vol 72
(4)
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pp. 2999-3004
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1996 ◽
Vol 70
(11)
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pp. 7974-7983
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2000 ◽
Vol 74
(22)
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pp. 10846-10851
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2011 ◽
Vol 9
(2)
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pp. 179-193
1994 ◽
Vol 68
(10)
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pp. 6794-6798
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