scholarly journals Unusual Antigenic and Genetic Characteristics of Human Respiratory Syncytial Viruses Isolated in Cuba

1998 ◽  
Vol 72 (9) ◽  
pp. 7589-7592 ◽  
Author(s):  
Odalys Valdés ◽  
Isidoro Martínez ◽  
Angel Valdivia ◽  
Reynel Cancio ◽  
Clara Savón ◽  
...  
Keyword(s):  
G Protein ◽  
Protein Gene ◽  
Temperate Climate ◽  
Nucleotide Difference ◽  
Genetic Characteristics ◽  
Socioeconomic Characteristics ◽  
Genetic Level ◽  
Respiratory Syncytial Viruses ◽  
Syncytial Virus ◽  
Extra Nucleotide

ABSTRACT The G protein of 23 strains of human respiratory syncytial virus isolated in Havana, Cuba, between October 1994 and January 1995 was analyzed at the antigenic and genetic level. All viruses reacted with 10 of 11 antibodies specific for the Long strain. Moreover, the G protein gene of the Cuban isolates had only five nucleotide differences from the sequence of the Long gene. The homogeneity of the Cuban isolates and their resemblance to an ancient strain, such as Long, are at odds with previous findings for viruses isolated in countries with a temperate climate and different socioeconomic status. The G proteins of three of four other viruses isolated in Havana 2 years later (1996) were also identical to those of the 1994-to-1995 isolates, and the fourth virus had a single extra nucleotide difference. This, again, is unusual, since no identical viruses had been isolated in different epidemics previously. The singular characteristics of the Cuban isolates reported here are discussed in terms of the epidemiological, climatic, and socioeconomic characteristics of Cuba.

scholarly journals Positive Selection Results in Frequent Reversible Amino Acid Replacements in the G Protein Gene of Human Respiratory Syncytial Virus

2009 ◽  
Vol 5 (1) ◽  
pp. e1000254 ◽  
Author(s):  
Viviane F. Botosso ◽  
Paolo M. de A. Zanotto ◽  
Mirthes Ueda ◽  
Eurico Arruda ◽  
Alfredo E. Gilio ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Amino Acid ◽  
Positive Selection ◽  
G Protein ◽  
Protein Gene ◽  
Human Respiratory Syncytial Virus ◽  
Syncytial Virus

scholarly journals Intrapatient Variation of the Respiratory Syncytial Virus Attachment Protein Gene

2010 ◽  
Vol 84 (19) ◽  
pp. 10425-10428 ◽  
Author(s):  
Charles N. Agoti ◽  
Jean L. Mbisa ◽  
Ann Bett ◽  
Graham F. Medley ◽  
D. James Nokes ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
G Protein ◽  
Protein Gene ◽  
Point Mutations ◽  
Virus Attachment ◽  
Single Genome ◽  
B Virus ◽  
Intrapatient Variability ◽  
Syncytial Virus ◽  
Group B

ABSTRACT Intrapatient variability of the attachment (G) protein gene of respiratory syncytial virus (RSV) was examined using both population and single-genome sequencing. Samples from three patients infected with a group B virus variant which has a 60-nucleotide duplication in the G protein gene were examined. These samples were chosen because occasional mixed sequence bases were observed. In a minority of RSV genomes from these patients considerable variability was found, including point mutations, insertions, and deletions. Of particular note, the deletion of the exact portion of the gene which had been duplicated in some isolates was observed in viral RNAs from two patients.

Genetic variability in the G protein gene of human respiratory syncytial virus isolated from the Campinas metropolitan region, Brazil

2008 ◽  
Vol 80 (9) ◽  
pp. 1653-1660 ◽  
Author(s):  
Luciana Helena Antoniassi da Silva ◽  
Fernando Rosado Spilki ◽  
Adriana Gut Lopes Riccetto ◽  
Renata Servan de Almeida ◽  
Emílio Carlos Elias Baracat ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Genetic Variability ◽  
G Protein ◽  
Protein Gene ◽  
Human Respiratory Syncytial Virus ◽  
Metropolitan Region ◽  
Syncytial Virus

scholarly journals Genetic variability among group A and B respiratory syncytial viruses in Mozambique: identification of a new cluster of group B isolates

2001 ◽  
Vol 82 (1) ◽  
pp. 103-111 ◽  
Author(s):  
Anna Roca ◽  
Mari-Paz Loscertales ◽  
Llorenç Quintó ◽  
Pilar Pérez-Breña ◽  
Neide Vaz ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Protein Gene ◽  
N Protein ◽  
Glycosylation Sites ◽  
Rsv Infection ◽  
Group A ◽  
Respiratory Syncytial Viruses ◽  
Syncytial Virus ◽  
Group B ◽  
Vulnerable Adults

Respiratory syncytial virus (RSV) is the major cause of acute lower respiratory tract infection in children and vulnerable adults, but little is known regarding RSV infection in Africa. In this report, a recent RSV outbreak in Mozambique was studied and results showed that 275 of 3192 (8·6%) nasopharyngeal aspirates tested were RSV-positive by ELISA. RSV presents two antigenic groups (A and B) with a high genetic and antigenic variability between and within them. Analysis by a new RFLP assay of RT–PCR amplified N protein gene products showed a higher prevalence of group B RSV than that of group A (85% versus 15%). However, genetic variability of the G protein gene was higher among group A RSV strains. The frequency and pattern of glycosylation sites were also quite different between both groups. In addition, two different phylogenetic clusters of Mozambican viruses were found within each group, but only sequences from cluster B-I were relatively distinct from previously described isolates. The implications of such differences in the antigenic and immunogenic characteristics of each group are discussed.

Nosocomial outbreak of respiratory syncytial virus subgroup B variants with the 60 nucleotides-duplicated G protein gene

2004 ◽  
Vol 74 (1) ◽  
pp. 161-165 ◽  
Author(s):  
Kazushige Nagai ◽  
Hotaka Kamasaki ◽  
Yuki Kuroiwa ◽  
Lisa Okita ◽  
Hiroyuki Tsutsumi
Keyword(s):  
Respiratory Syncytial Virus ◽  
G Protein ◽  
Protein Gene ◽  
Nosocomial Outbreak ◽  
Syncytial Virus

scholarly journals Ten Years of Global Evolution of the Human Respiratory Syncytial Virus BA Genotype with a 60-Nucleotide Duplication in the G Protein Gene

2010 ◽  
Vol 84 (15) ◽  
pp. 7500-7512 ◽  
Author(s):  
Alfonsina Trento ◽  
Inmaculada Casas ◽  
Ana Calderón ◽  
Maria L. Garcia-Garcia ◽  
Cristina Calvo ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
G Protein ◽  
Protein Gene ◽  
Natural Host ◽  
Human Respiratory Syncytial Virus ◽  
Clinical Samples ◽  
New Genotype ◽  
Syncytial Virus ◽  
Group B ◽  
Ba Genotype

ABSTRACT The emergence of natural isolates of human respiratory syncytial virus group B (HRSV-B) with a 60-nucleotide (nt) duplication in the G protein gene in Buenos Aires, Argentina, in 1999 (A. Trento et al., J. Gen. Virol. 84:3115-3120, 2003) and their dissemination worldwide allowed us to use the duplicated segment as a natural tag to examine in detail the evolution of HRSV during propagation in its natural host. Viruses with the duplicated segment were all clustered in a new genotype, named BA (A. Trento et al., J. Virol. 80:975-984, 2006). To obtain information about the prevalence of these viruses in Spain, we tested for the presence of the duplicated segment in positive HRSV-B clinical samples collected at the Severo Ochoa Hospital (Madrid) during 12 consecutive epidemics (1996-1997 to 2007-2008). Viruses with the 60-nt duplication were found in 61 samples, with a high prevalence relative to the rest of B genotypes in the most recent seasons. Global phylogenetic and demographic analysis of all G sequences containing the duplication, collected across five continents up until April 2009, revealed that the prevalence of the BA genotype increased gradually until 2004-2005, despite its rapid dissemination worldwide. After that date and coinciding with a bottleneck effect on the population size, a relatively new BA lineage (BA-IV) replaced all other group B viruses, suggesting further adaptation of the BA genotype to its natural host.

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