scholarly journals Nonrandom Distribution of Hepatitis C Virus Quasispecies in Plasma and Peripheral Blood Mononuclear Cell Subsets

1999 ◽  
Vol 73 (11) ◽  
pp. 9213-9221 ◽  
Author(s):  
Anne Marie Roque Afonso ◽  
Jiaji Jiang ◽  
François Penin ◽  
Claire Tareau ◽  
Didier Samuel ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Cell ◽  
Hcv Rna ◽  
Structural Constraints ◽  
Nucleotide Substitutions ◽  
Peripheral Blood Mononuclear ◽  
Cellular Compartments

ABSTRACT The existence of an extrahepatic reservoir of hepatitis C virus (HCV) is suggested by differences in quasispecies composition between the liver, peripheral blood mononuclear cells, and serum. We studied HCV RNA compartmentalization in the plasma of nine patients, in CD19+, CD8+, and CD4+ positively selected cells, and also in the negatively selected cell fraction (NF). HCV RNA was detected in all plasma samples, in seven of nine CD19+, three of eight CD8+, and one of nine CD4+ cell samples, and in seven of eight NF cells. Cloning and sequencing of HVR1 in two patients showed a sequence grouping: quasispecies from a given compartment (all studied compartments for one patient and CD8+ and NF for the other) were statistically more genetically like each other than like quasispecies from any other compartment. The characteristics of amino acid and nucleotide substitutions suggested the same structural constraints on HVR1, even in very divergent strains from the cellular compartments, and homogeneous selection pressure on the different compartments. These findings demonstrate the compartmental distribution of HCV quasispecies within peripheral blood cell subsets and have important implications for the study of extrahepatic HCV replication and interaction with the immune system.

scholarly journals Continuous release of hepatitis C virus (HCV) by peripheral blood mononuclear cells and B-lymphoblastoid cell-line cultures derived from HCV-infected patients

2005 ◽  
Vol 86 (6) ◽  
pp. 1717-1727 ◽  
Author(s):  
Patricia Baré ◽  
Ivana Massud ◽  
Cecilia Parodi ◽  
Liliana Belmonte ◽  
Gabriel García ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Hcv Rna ◽  
Peripheral Blood Mononuclear ◽  
Pbmc Culture ◽  
Cell Counts ◽  
Blood Mononuclear Cells

In order to investigate hepatitis C virus (HCV) persistence and replication in peripheral blood mononuclear cells (PBMC) from a group of haemophilic individuals, HCV production and release to PBMC culture supernatants (SNs) from HCV singly infected patients and HIV/HCV co-infected patients was studied. HCV RNA+ SNs were found more frequently from HIV/HCV co-infected individuals (89·5 %) with poor reconstitution of their immune status than from singly HCV-infected patients (57 %) or from HIV/HCV co-infected individuals with a good response to highly active anti-retroviral therapy (50 %). The presence of the HCV genome in culture SNs was associated with lower CD4+ T-cell counts and with a more severe clinical picture of HIV infection. In spite of prolonged negative HCV viraemia, PBMC from HIV/HCV co-infected patients released the HCV genome after culture. HCV permissive PBMC allowed generation of HCV productive B cell lines with continuous HCV replication. These findings add further weight to the involvement of PBMCs in persistence of HCV infection and emphasize the role of B lymphocytes as HCV reservoirs.

scholarly journals Detection of hepatitis C virus RNA in peripheral blood mononuclear cells of infected patients by in situ hybridization

Blood ◽  
1994 ◽  
Vol 83 (1) ◽  
pp. 269-273 ◽  
Author(s):  
J Moldvay ◽  
P Deny ◽  
S Pol ◽  
C Brechot ◽  
E Lamas
Keyword(s):  
In Situ Hybridization ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Hcv Rna ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

We used in situ hybridization to detect hepatitis C virus (HCV) infection of peripheral blood mononuclear cells (PBMNC) from 11 patients with chronic active hepatitis. Using 35S-labeled HCV-RNA probe, HCV-RNA-positive and -negative strands were observed in unstimulated PBMNC from three patients, all of whom were receiving immunosuppressive drugs after orthotopic liver transplantation (OLT). HCV-RNA sequences were also identified in PBMNC from three patients who were not undergoing immunosuppression, after stimulation with either phytohemagglutinin (PHA) or pokeweed mitogen (PWM). In contrast, HCV- RNA was not found in the remaining five patients, who had not undergone OLT and whose cells were not stimulated with mitogens. These results show that mononuclear cells can be infected by HCV and that mitogenic stimulation of infected cells increases HCV-RNA replication.

scholarly journals Infection of peripheral blood mononuclear cells by hepatitis C virus in mixed cryoglobulinemia

Blood ◽  
1993 ◽  
Vol 82 (12) ◽  
pp. 3701-3704 ◽  
Author(s):  
C Ferri ◽  
M Monti ◽  
L La Civita ◽  
G Longombardo ◽  
F Greco ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Mixed Cryoglobulinemia ◽  
Hcv Infection ◽  
Hcv Rna ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

A striking association between hepatitis C virus (HCV) infection and mixed cryoglobulinemia (MC) has been shown; thus, HCV seems to play an important etiopathogenetic role in this lymphoproliferative disorder. Because HCV is both a hepatotropic and lymphotropic virus, this study aimed to investigate the prevalence of HCV infection of peripheral blood mononuclear cells (PBMCs) in a series of 16 patients with type II (IgMk) MC. Antibodies against HCV were detected by commercially available kits (Second Generation Chiron enzyme-linked immunosorbent assay [ELISA] and recombinant-based immunoblot assay [RIBA]), and the presence of HCV RNA was evaluated in both sera and isolated PBMCs using the polymerase chain reaction technique. A previous exposure to HCV was shown by ELISA and confirmed by RIBA in all cases (100%). Moreover, HCV RNA was present in the sera of 8 of 16 patients (50%), whereas its frequency markedly increased (13 of 16 [81%]) when genomic sequences were detected in peripheral lymphocytes. HCV RNA was never detected in the PBMCs of 20 control subjects. These findings showed that HCV infection, alone or in combination with other factors, may be responsible for the clonal B-cell expansion underlying the systemic manifestations of MC, and may explain the appearance of a malignant non- Hodgkin's lymphoma in some subjects.

scholarly journals Detection of hepatitis C virus RNA in peripheral blood mononuclear cells of infected patients by in situ hybridization

Blood ◽  
1994 ◽  
Vol 83 (1) ◽  
pp. 269-273 ◽  
Author(s):  
J Moldvay ◽  
P Deny ◽  
S Pol ◽  
C Brechot ◽  
E Lamas
Keyword(s):  
In Situ Hybridization ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Hcv Rna ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Abstract We used in situ hybridization to detect hepatitis C virus (HCV) infection of peripheral blood mononuclear cells (PBMNC) from 11 patients with chronic active hepatitis. Using 35S-labeled HCV-RNA probe, HCV-RNA-positive and -negative strands were observed in unstimulated PBMNC from three patients, all of whom were receiving immunosuppressive drugs after orthotopic liver transplantation (OLT). HCV-RNA sequences were also identified in PBMNC from three patients who were not undergoing immunosuppression, after stimulation with either phytohemagglutinin (PHA) or pokeweed mitogen (PWM). In contrast, HCV- RNA was not found in the remaining five patients, who had not undergone OLT and whose cells were not stimulated with mitogens. These results show that mononuclear cells can be infected by HCV and that mitogenic stimulation of infected cells increases HCV-RNA replication.

scholarly journals Infection of peripheral blood mononuclear cells by hepatitis C virus in mixed cryoglobulinemia

Blood ◽  
1993 ◽  
Vol 82 (12) ◽  
pp. 3701-3704 ◽  
Author(s):  
C Ferri ◽  
M Monti ◽  
L La Civita ◽  
G Longombardo ◽  
F Greco ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Mixed Cryoglobulinemia ◽  
Hcv Infection ◽  
Hcv Rna ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Abstract A striking association between hepatitis C virus (HCV) infection and mixed cryoglobulinemia (MC) has been shown; thus, HCV seems to play an important etiopathogenetic role in this lymphoproliferative disorder. Because HCV is both a hepatotropic and lymphotropic virus, this study aimed to investigate the prevalence of HCV infection of peripheral blood mononuclear cells (PBMCs) in a series of 16 patients with type II (IgMk) MC. Antibodies against HCV were detected by commercially available kits (Second Generation Chiron enzyme-linked immunosorbent assay [ELISA] and recombinant-based immunoblot assay [RIBA]), and the presence of HCV RNA was evaluated in both sera and isolated PBMCs using the polymerase chain reaction technique. A previous exposure to HCV was shown by ELISA and confirmed by RIBA in all cases (100%). Moreover, HCV RNA was present in the sera of 8 of 16 patients (50%), whereas its frequency markedly increased (13 of 16 [81%]) when genomic sequences were detected in peripheral lymphocytes. HCV RNA was never detected in the PBMCs of 20 control subjects. These findings showed that HCV infection, alone or in combination with other factors, may be responsible for the clonal B-cell expansion underlying the systemic manifestations of MC, and may explain the appearance of a malignant non- Hodgkin's lymphoma in some subjects.

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