Interaction of Eukaryotic Initiation Factor eIF4B with the Internal Ribosome Entry Site of Foot-and-Mouth Disease Virus Is Independent of the Polypyrimidine Tract-Binding Protein
1999 ◽
Vol 73
(7)
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pp. 6111-6113
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Keyword(s):
ABSTRACT Eukaryotic translation initiation factor 4B (eIF4B) binds directly to the internal ribosome entry site (IRES) of foot-and-mouth disease virus (FMDV). Mutations in all three subdomains of the IRES stem-loop 4 reduce binding of eIF4B and translation efficiency in parallel, indicating that eIF4B is functionally involved in FMDV translation initiation. In reticulocyte lysate devoid of polypyrimidine tract-binding protein (PTB), eIF4B still bound well to the wild-type IRES, even after removal of the major PTB-binding site. In conclusion, the interaction of eIF4B with the FMDV IRES is essential for IRES function but independent of PTB.
1997 ◽
Vol 71
(11)
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pp. 8330-8339
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2001 ◽
Vol 82
(4)
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pp. 757-763
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1996 ◽
Vol 24
(14)
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pp. 2697-2700
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2002 ◽
Vol 76
(19)
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pp. 9686-9694
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2004 ◽
Vol 85
(9)
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pp. 2555-2565
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1998 ◽
Vol 72
(8)
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pp. 6362-6372
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