Poliovirus Requires a Precise 5′ End for Efficient Positive-Strand RNA Synthesis
2000 ◽
Vol 74
(14)
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pp. 6394-6400
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ABSTRACT Poliovirus infectious RNA can be synthesized in vitro using phage DNA-dependent RNA-polymerases. These synthetic transcripts contain several extra nucleotides at the 5′ end, which are deleted during replication to generate authentic viral genomes. We removed those 5′-end extra nucleotides utilizing a hammerhead ribozyme to produce transcripts with accurate 5′ ends. These transcripts replicate substantially more rapidly in cell culture, demonstrating no lag before replication; they also replicate more efficiently in Xenopus laevis oocytes and in in vitro translation-replication cell extracts. In both systems, an exact 5′ end is necessary for synthesis of positive-strand RNA but not negative-strand RNA.
2004 ◽
Vol 78
(23)
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pp. 13007-13018
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2006 ◽
Vol 87
(1)
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pp. 103-113
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2003 ◽
Vol 77
(23)
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pp. 12679-12691
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2002 ◽
Vol 76
(22)
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pp. 11321-11328
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2005 ◽
Vol 86
(3)
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pp. 707-718
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2005 ◽
Vol 79
(6)
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pp. 3254-3266
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1981 ◽
Vol 256
(9)
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pp. 4138-4141
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