Characterization of a Human Cytomegalovirus with Phosphorylation Site Mutations in the Immediate-Early 2 Protein
2002 ◽
Vol 76
(2)
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pp. 928-932
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ABSTRACT A human cytomegalovirus mutant (TNsubIE2P) was constructed with alanine substitutions of four residues (T27, S144, T233, and S234) previously shown to be phosphorylated in the immediate-early 2 (IE2) protein. This mutant grew as well as the wild type at both low and high multiplicities of infection. The mutant activated the major immediate-early, UL4, and UL44 promoters to similar levels, and with similar kinetics, as wild-type virus. However, the TNsubIE2P mutant virus transactivated an endogenous simian virus 40 early promoter 4 h earlier and to higher levels than the wild-type virus in infected human fibroblasts. The modification of the IE2 protein by SUMO-1 (i.e., its sumoylated state) was also examined.
2002 ◽
Vol 76
(3)
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pp. 1043-1050
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2012 ◽
Vol 93
(4)
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pp. 716-721
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1978 ◽
Vol 25
(3)
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pp. 860-870
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1979 ◽
Vol 100
(1)
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pp. 51-60
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2005 ◽
Vol 79
(17)
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pp. 11022-11034
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