Replication of Bovine Papillomavirus Type 1 (BPV-1) DNA in Saccharomyces cerevisiae following Infection with BPV-1 Virions

ABSTRACT Saccharomyces cerevisiae protoplasts exposed to bovine papillomavirus type 1 (BPV-1) virions demonstrated uptake of virions on electron microscopy. S. cerevisiae cells looked larger after exposure to BPV-1 virions, and cell wall regeneration was delayed. Southern blot hybridization of Hirt DNA from cells exposed to BPV-1 virions demonstrated BPV-1 DNA, which could be detected over 80 days of culture and at least 13 rounds of division. Two-dimensional gel analysis of Hirt DNA showed replicative intermediates, confirming that the BPV-1 genome was replicating within S. cerevisiae. Nicked circle, linear, and supercoiled BPV-1 DNA species were observed in Hirt DNA preparations from S. cerevisiae cells infected for over 50 days, and restriction digestion showed fragments hybridizing to BPV-1 in accord with the predicted restriction map for circular BPV-1 episomes. These data suggest that BPV-1 can infect S. cerevisiae and that BPV-1 episomes can replicate in the infected S. cerevisiae cells.

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DNA methylation at the DMPK gene locus is associated with cognitive functions in myotonic dystrophy type 1

Epigenomics ◽

10.2217/epi-2020-0328 ◽

2020 ◽

Vol 12 (23) ◽

pp. 2051-2064

Author(s):

Édith Breton ◽

Cécilia Légaré ◽

Gayle Overend ◽

Simon-Pierre Guay ◽

Darren Monckton ◽

...

Keyword(s):

Dna Methylation ◽

Myotonic Dystrophy ◽

Cognitive Functions ◽

Gene Locus ◽

Blot Hybridization ◽

Southern Blot Hybridization ◽

Myotonic Dystrophy Type 1 ◽

Myotonic Dystrophy Type ◽

Dmpk Gene

Aim: Myotonic dystrophy type 1 (DM1) is caused by an unstable trinucleotide (CTG) expansion at the DMPK gene locus. Cognitive dysfunctions are often observed in the condition. We investigated the association between DMPK blood DNA methylation (DNAm) and cognitive functions in DM1, considering expansion length and variant repeats (VRs). Method: Data were obtained from 115 adult-onset DM1 patients. Molecular analyses consisted of pyrosequencing, small pool PCR and Southern blot hybridization. Cognitive functions were assessed by validated neuropsychological tests. Results: For patients without VRs (n = 103), blood DNAm at baseline independently contributed to predict cognitive functions 9 years later. Patients with VRs (n = 12) had different DNAm and cognitive profiles. Conclusion: DNAm allows to better understand DM1-related cognitive dysfunction etiology.

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Saccharomyces cerevisiae Is Permissive for Replication of Bovine Papillomavirus Type 1

Journal of Virology ◽

10.1128/jvi.76.23.12265-12273.2002 ◽

2002 ◽

Vol 76 (23) ◽

pp. 12265-12273 ◽

Cited By ~ 24

Author(s):

Kong-Nan Zhao ◽

Ian H. Frazer

Keyword(s):

Saccharomyces Cerevisiae ◽

Full Length ◽

Capsid Proteins ◽

Bovine Papillomavirus ◽

Dna Virus ◽

Viral Dna ◽

Virus Like Particles ◽

Virus Particles ◽

Episomal Dna

ABSTRACT We recently demonstrated that Saccharomyces cerevisiae protoplasts can take up bovine papillomavirus type 1 (BPV1) virions and that viral episomal DNA is replicated after uptake. Here we demonstrate that BPV virus-like particles are assembled in infected S. cerevisiae cultures from newly synthesized capsid proteins and also package newly synthesized DNA, including full-length and truncated viral DNA and S. cerevisiae-derived DNA. Virus particles prepared in S. cerevisiae are able to convey packaged DNA to Cos1 cells and to transform C127 cells. Infectivity was blocked by antisera to BPV1 L1 but not antisera to BPV1 E4. We conclude that S. cerevisiae is permissive for the replication of BPV1 virus.

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A Restriction Map of Bovine Papillomavirus Type-1 DNA

Journal of General Virology ◽

10.1099/0022-1317-56-1-213 ◽

1981 ◽

Vol 56 (1) ◽

pp. 213-217

Author(s):

D. M. Morgan ◽

M. F. Murphy ◽

J. M. Abraham ◽

W. J. Meinke

Keyword(s):

Restriction Map ◽

Bovine Papillomavirus

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A model for long term infection of bovine papillomavirus type 1 in Saccharomyces cerevisiae

Acta Virologica ◽

10.4149/av_2021_204 ◽

2021 ◽

Author(s):

Jun Chen ◽

Qijia Li ◽

Weixu Feng ◽

Zhuo Chen ◽

Qi Wang ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Bovine Papillomavirus

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Characterization of Episomal Replication of Bovine Papillomavirus Type 1 DNA in Long-Term Virion-Infected Saccharomyces Cerevisiae Culture

Virologica Sinica ◽

10.1007/s12250-021-00439-y ◽

2021 ◽

Author(s):

Quanmei Tu ◽

Weixu Feng ◽

Zhuo Chen ◽

Qijia Li ◽

Yu Zhao ◽

...

Keyword(s):

Time Course ◽

Blot Hybridization ◽

Yeast Cells ◽

Bovine Papillomavirus ◽

Two Dimensional Gel Electrophoresis ◽

Yeast Cultures ◽

Active Replication ◽

Replication Intermediates

AbstractWe have previously reported that bovine papillomavirus type 1 (BPV-1) DNA can replicate its genome and produce infectious virus-like particles in short term virion-infected S. cerevisiae (budding yeast) cultures (Zhao and Frazer 2002, Journal of Virology, 76:3359–64 and 76:12265–73). Here, we report the episomal replications of BPV-1 DNA in long term virion-infected S. cerevisiae culture up to 108 days. Episomal replications of the BPV-1 DNA could be divided into three patterns at three stages, early active replication (day 3–16), middle weak replication (day 23–34/45) and late stable replication (day 45–82). Two-dimensional gel electrophoresis analysis and Southern blot hybridization have revealed further that multiple replication intermediates of BPV-1 DNA including linear form, stranded DNA, monomers and higher oligomers were detected in the virion-infected yeast cells over the time course. Higher oligomers shown as covalently closed circular DNAs (cccDNAs) are the most important replication intermediates that serve as the main nuclear transcription template for producing all viral RNAs in the viral life cycle. In this study, the cccDNAs were generated at the early active replication stage with the highest frequencies and then at late stable replication, but they appeared to be suppressed at the middle weak replication. Our data provided a novel insight that BPV-1 genomic DNA could replicate episomally for the long period and produce the key replication intermediates cccDNAs in S. cerevisiae system.

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